Diamond nanoparticles downregulate expression of CycD and CycE in glioma cells

Marta Grodzik; Jaroslaw Szczepaniak; Barbara Strojny-Cieslak; Anna Hotowy; Mateusz Wierzbicki; Slawomir Jaworski; Marta Kutwin; Emilia Soltan; Tomasz Mandat; Aneta Lewicka; Andre Chwalibog

doi:10.3390/molecules24081549

Diamond nanoparticles downregulate expression of CycD and CycE in glioma cells

Marta Grodzik^*, Jaroslaw Szczepaniak, Barbara Strojny-Cieslak, Anna Hotowy, Mateusz Wierzbicki, Slawomir Jaworski, Marta Kutwin, Emilia Soltan, Tomasz Mandat, Aneta Lewicka, Andre Chwalibog

^*Corresponding author for this work

2 Citations (Scopus)

17 Downloads (Pure)

Abstract

Our previous studies have shown that diamond nanoparticles (NDs) exhibited antiangiogenic and proapoptotic properties in vitro in glioblastoma multiforme (GBM) cells and in tumors in vivo. Moreover, NDs inhibited adhesion, leading to the suppression of migration and invasion of GBM. In the present study, we hypothesized that the NDs might also inhibit proliferation and cell cycle in glioma cells. Experiments were performed in vitro with the U87 and U118 lines of GBM cells, and for comparison, the Hs5 line of stromal cells (normal cells) after 24 h and 72 h of treatment. The analyses included cell morphology, cell death, viability, and cell cycle analysis, double timing assay, and gene expression (Rb, E2F1, CycA, CycB, CycD, CycE, PTEN, Ki-67). After 72 h of ND treatment, the expression level of Rb, CycD, and CycE in the U118 cells, and E2F1, CycD, and CycE in the U87 cells were significantly lower in comparison to those in the control group. We observed that decreased expression of cyclins inhibited the G1/S phase transition, arresting the cell cycle in the G0/G1 phase in glioma cells. The NDs did not affect the cell cycle as well as PTEN and Ki-67 expression in normal cells (Hs5), although it can be assumed that the NDs reduced proliferation and altered the cell cycle in fast dividing cells.

Original language	English
Article number	1549
Journal	Molecules
Number of pages	16
ISSN	1420-3049
DOIs	https://doi.org/10.3390/molecules24081549
Publication status	Published - 2019

Keywords

Cancer
Cell cycle
Diamond nanoparticles
Glioblastoma
Proliferation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Diamond Nanoparticles Downregulate Expression of CycD and CycE in Glioma CellsFinal published version, 4.49 MBLicence: CC BY

Cite this

@article{2236b2ef296a46c49fffbfc306e532b3,

title = "Diamond nanoparticles downregulate expression of CycD and CycE in glioma cells",

abstract = "Our previous studies have shown that diamond nanoparticles (NDs) exhibited antiangiogenic and proapoptotic properties in vitro in glioblastoma multiforme (GBM) cells and in tumors in vivo. Moreover, NDs inhibited adhesion, leading to the suppression of migration and invasion of GBM. In the present study, we hypothesized that the NDs might also inhibit proliferation and cell cycle in glioma cells. Experiments were performed in vitro with the U87 and U118 lines of GBM cells, and for comparison, the Hs5 line of stromal cells (normal cells) after 24 h and 72 h of treatment. The analyses included cell morphology, cell death, viability, and cell cycle analysis, double timing assay, and gene expression (Rb, E2F1, CycA, CycB, CycD, CycE, PTEN, Ki-67). After 72 h of ND treatment, the expression level of Rb, CycD, and CycE in the U118 cells, and E2F1, CycD, and CycE in the U87 cells were significantly lower in comparison to those in the control group. We observed that decreased expression of cyclins inhibited the G1/S phase transition, arresting the cell cycle in the G0/G1 phase in glioma cells. The NDs did not affect the cell cycle as well as PTEN and Ki-67 expression in normal cells (Hs5), although it can be assumed that the NDs reduced proliferation and altered the cell cycle in fast dividing cells.",

keywords = "Cancer, Cell cycle, Diamond nanoparticles, Glioblastoma, Proliferation",

author = "Marta Grodzik and Jaroslaw Szczepaniak and Barbara Strojny-Cieslak and Anna Hotowy and Mateusz Wierzbicki and Slawomir Jaworski and Marta Kutwin and Emilia Soltan and Tomasz Mandat and Aneta Lewicka and Andre Chwalibog",

year = "2019",

doi = "10.3390/molecules24081549",

language = "English",

journal = "Molecules",

issn = "1420-3049",

publisher = "M D P I AG",

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TY - JOUR

T1 - Diamond nanoparticles downregulate expression of CycD and CycE in glioma cells

AU - Grodzik, Marta

AU - Szczepaniak, Jaroslaw

AU - Strojny-Cieslak, Barbara

AU - Hotowy, Anna

AU - Wierzbicki, Mateusz

AU - Jaworski, Slawomir

AU - Kutwin, Marta

AU - Soltan, Emilia

AU - Mandat, Tomasz

AU - Lewicka, Aneta

AU - Chwalibog, Andre

PY - 2019

Y1 - 2019

N2 - Our previous studies have shown that diamond nanoparticles (NDs) exhibited antiangiogenic and proapoptotic properties in vitro in glioblastoma multiforme (GBM) cells and in tumors in vivo. Moreover, NDs inhibited adhesion, leading to the suppression of migration and invasion of GBM. In the present study, we hypothesized that the NDs might also inhibit proliferation and cell cycle in glioma cells. Experiments were performed in vitro with the U87 and U118 lines of GBM cells, and for comparison, the Hs5 line of stromal cells (normal cells) after 24 h and 72 h of treatment. The analyses included cell morphology, cell death, viability, and cell cycle analysis, double timing assay, and gene expression (Rb, E2F1, CycA, CycB, CycD, CycE, PTEN, Ki-67). After 72 h of ND treatment, the expression level of Rb, CycD, and CycE in the U118 cells, and E2F1, CycD, and CycE in the U87 cells were significantly lower in comparison to those in the control group. We observed that decreased expression of cyclins inhibited the G1/S phase transition, arresting the cell cycle in the G0/G1 phase in glioma cells. The NDs did not affect the cell cycle as well as PTEN and Ki-67 expression in normal cells (Hs5), although it can be assumed that the NDs reduced proliferation and altered the cell cycle in fast dividing cells.

AB - Our previous studies have shown that diamond nanoparticles (NDs) exhibited antiangiogenic and proapoptotic properties in vitro in glioblastoma multiforme (GBM) cells and in tumors in vivo. Moreover, NDs inhibited adhesion, leading to the suppression of migration and invasion of GBM. In the present study, we hypothesized that the NDs might also inhibit proliferation and cell cycle in glioma cells. Experiments were performed in vitro with the U87 and U118 lines of GBM cells, and for comparison, the Hs5 line of stromal cells (normal cells) after 24 h and 72 h of treatment. The analyses included cell morphology, cell death, viability, and cell cycle analysis, double timing assay, and gene expression (Rb, E2F1, CycA, CycB, CycD, CycE, PTEN, Ki-67). After 72 h of ND treatment, the expression level of Rb, CycD, and CycE in the U118 cells, and E2F1, CycD, and CycE in the U87 cells were significantly lower in comparison to those in the control group. We observed that decreased expression of cyclins inhibited the G1/S phase transition, arresting the cell cycle in the G0/G1 phase in glioma cells. The NDs did not affect the cell cycle as well as PTEN and Ki-67 expression in normal cells (Hs5), although it can be assumed that the NDs reduced proliferation and altered the cell cycle in fast dividing cells.

KW - Cancer

KW - Cell cycle

KW - Diamond nanoparticles

KW - Glioblastoma

KW - Proliferation

U2 - 10.3390/molecules24081549

DO - 10.3390/molecules24081549

M3 - Journal article

C2 - 31010146

AN - SCOPUS:85064879196

SN - 1420-3049

JO - Molecules

JF - Molecules

M1 - 1549

ER -