Dexamethasone up-regulates skeletal muscle maximal Na+,K+ pump activity by muscle group specific mechanisms in humans

Nikolai Nordsborg; Craig Goodmann; Michael J. McKenna; Jens Bangsbo

doi:10.1113/jphysiol.2005.087031

Dexamethasone up-regulates skeletal muscle maximal Na⁺,K⁺ pump activity by muscle group specific mechanisms in humans

Nikolai Nordsborg, Craig Goodmann, Michael J. McKenna, Jens Bangsbo

24 Citations (Scopus)

Abstract

Dexamethasone, a widely clinically used glucocorticoid, increaseshuman skeletal muscle Na⁺,K⁺ pump content, but the effects onmaximal Na⁺,K⁺ pump activity and subunit specific mRNA are unknown.Ten healthy male subjects ingested dexamethasone for 5 daysand the effects on Na⁺,K⁺ pump content, maximal activity andsubunit specific mRNA level (a1, a2, ß1, ß2,ß3) in deltoid and vastus lateralis muscle were investigated.Before treatment, maximal Na⁺,K⁺ pump activity, as well as a1,a2, ß1 and ß2 mRNA levels were higher (P< 0.05) in vastus lateralis than in deltoid. Dexamethasonetreatment increased Na⁺,K⁺ pump maximal activity in vastus lateralisand deltoid by 14 ± 7% (P < 0.05) and 18 ±6% (P < 0.05) as well as Na⁺,K⁺ pump content by 18 ±9% (P < 0.001) and 24 ± 8% (P < 0.01), respectively.Treatment with dexamethasone resulted in a higher a1, a2, ß1and ß2 mRNA expression in the deltoid (P < 0.05),but no effects on Na⁺,K⁺ pump mRNA were detected in vastus lateralis.In conclusion, dexamethasone treatment increased maximal Na⁺,K⁺pump activity in both vastus lateralis and deltoid muscles.The relative importance of transcription and translation inthe glucocorticoid-induced regulation of Na⁺,K⁺ pump expressionseems to be muscle specific and possibly dependent on the actualtraining condition of the muscle, such that a high Na⁺,K⁺ pumpmaximal activity and mRNA level prior to treatment preventsthe transcriptional response to dexamethasone, but not the increasein Na⁺,K⁺ pump content and maximal activity.

Original language	English
Journal	Journal of Physiology
Volume	567
Issue number	2
Pages (from-to)	583-589
Number of pages	7
ISSN	0022-3751
DOIs	https://doi.org/10.1113/jphysiol.2005.087031
Publication status	Published - 2005

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@article{d35b4240a55811dbbee902004c4f4f50,

title = "Dexamethasone up-regulates skeletal muscle maximal Na+,K+ pump activity by muscle group specific mechanisms in humans",

abstract = "Dexamethasone, a widely clinically used glucocorticoid, increases human skeletal muscle Na+,K+ pump content, but the effects on maximal Na+,K+ pump activity and subunit specific mRNA are unknown. Ten healthy male subjects ingested dexamethasone for 5 days and the effects on Na+,K+ pump content, maximal activity and subunit specific mRNA level (a1, a2, {\ss}1, {\ss}2, {\ss}3) in deltoid and vastus lateralis muscle were investigated. Before treatment, maximal Na+,K+ pump activity, as well as a1, a2, {\ss}1 and {\ss}2 mRNA levels were higher (P < 0.05) in vastus lateralis than in deltoid. Dexamethasone treatment increased Na+,K+ pump maximal activity in vastus lateralis and deltoid by 14 ± 7% (P < 0.05) and 18 ± 6% (P < 0.05) as well as Na+,K+ pump content by 18 ± 9% (P < 0.001) and 24 ± 8% (P < 0.01), respectively. Treatment with dexamethasone resulted in a higher a1, a2, {\ss}1 and {\ss}2 mRNA expression in the deltoid (P < 0.05), but no effects on Na+,K+ pump mRNA were detected in vastus lateralis. In conclusion, dexamethasone treatment increased maximal Na+,K+ pump activity in both vastus lateralis and deltoid muscles. The relative importance of transcription and translation in the glucocorticoid-induced regulation of Na+,K+ pump expression seems to be muscle specific and possibly dependent on the actual training condition of the muscle, such that a high Na+,K+ pump maximal activity and mRNA level prior to treatment prevents the transcriptional response to dexamethasone, but not the increase in Na+,K+ pump content and maximal activity. ",

author = "Nikolai Nordsborg and Craig Goodmann and McKenna, {Michael J.} and Jens Bangsbo",

note = "PUF 2005 5200 028",

year = "2005",

doi = "10.1113/jphysiol.2005.087031",

language = "English",

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TY - JOUR

T1 - Dexamethasone up-regulates skeletal muscle maximal Na+,K+ pump activity by muscle group specific mechanisms in humans

AU - Nordsborg, Nikolai

AU - Goodmann, Craig

AU - McKenna, Michael J.

AU - Bangsbo, Jens

N1 - PUF 2005 5200 028

PY - 2005

Y1 - 2005

N2 - Dexamethasone, a widely clinically used glucocorticoid, increases human skeletal muscle Na+,K+ pump content, but the effects on maximal Na+,K+ pump activity and subunit specific mRNA are unknown. Ten healthy male subjects ingested dexamethasone for 5 days and the effects on Na+,K+ pump content, maximal activity and subunit specific mRNA level (a1, a2, ß1, ß2, ß3) in deltoid and vastus lateralis muscle were investigated. Before treatment, maximal Na+,K+ pump activity, as well as a1, a2, ß1 and ß2 mRNA levels were higher (P < 0.05) in vastus lateralis than in deltoid. Dexamethasone treatment increased Na+,K+ pump maximal activity in vastus lateralis and deltoid by 14 ± 7% (P < 0.05) and 18 ± 6% (P < 0.05) as well as Na+,K+ pump content by 18 ± 9% (P < 0.001) and 24 ± 8% (P < 0.01), respectively. Treatment with dexamethasone resulted in a higher a1, a2, ß1 and ß2 mRNA expression in the deltoid (P < 0.05), but no effects on Na+,K+ pump mRNA were detected in vastus lateralis. In conclusion, dexamethasone treatment increased maximal Na+,K+ pump activity in both vastus lateralis and deltoid muscles. The relative importance of transcription and translation in the glucocorticoid-induced regulation of Na+,K+ pump expression seems to be muscle specific and possibly dependent on the actual training condition of the muscle, such that a high Na+,K+ pump maximal activity and mRNA level prior to treatment prevents the transcriptional response to dexamethasone, but not the increase in Na+,K+ pump content and maximal activity.

AB - Dexamethasone, a widely clinically used glucocorticoid, increases human skeletal muscle Na+,K+ pump content, but the effects on maximal Na+,K+ pump activity and subunit specific mRNA are unknown. Ten healthy male subjects ingested dexamethasone for 5 days and the effects on Na+,K+ pump content, maximal activity and subunit specific mRNA level (a1, a2, ß1, ß2, ß3) in deltoid and vastus lateralis muscle were investigated. Before treatment, maximal Na+,K+ pump activity, as well as a1, a2, ß1 and ß2 mRNA levels were higher (P < 0.05) in vastus lateralis than in deltoid. Dexamethasone treatment increased Na+,K+ pump maximal activity in vastus lateralis and deltoid by 14 ± 7% (P < 0.05) and 18 ± 6% (P < 0.05) as well as Na+,K+ pump content by 18 ± 9% (P < 0.001) and 24 ± 8% (P < 0.01), respectively. Treatment with dexamethasone resulted in a higher a1, a2, ß1 and ß2 mRNA expression in the deltoid (P < 0.05), but no effects on Na+,K+ pump mRNA were detected in vastus lateralis. In conclusion, dexamethasone treatment increased maximal Na+,K+ pump activity in both vastus lateralis and deltoid muscles. The relative importance of transcription and translation in the glucocorticoid-induced regulation of Na+,K+ pump expression seems to be muscle specific and possibly dependent on the actual training condition of the muscle, such that a high Na+,K+ pump maximal activity and mRNA level prior to treatment prevents the transcriptional response to dexamethasone, but not the increase in Na+,K+ pump content and maximal activity.

U2 - 10.1113/jphysiol.2005.087031

DO - 10.1113/jphysiol.2005.087031

M3 - Journal article

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SN - 0022-3751

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SP - 583

EP - 589

JO - Journal of Physiology

JF - Journal of Physiology

IS - 2