Developmental wiring of specific neurons is regulated by RET-1/Nogo-A in Caenorhabditis elegans

Nanna Torpe; Steffen Nørgaard; Anette M. Høye; Roger Pocock

doi:10.1534/genetics.115.185322

Developmental wiring of specific neurons is regulated by RET-1/Nogo-A in Caenorhabditis elegans

Nanna Torpe, Steffen Nørgaard, Anette M. Høye, Roger Pocock^*

^*Corresponding author for this work

2 Citations (Scopus)

Abstract

Nogo-A is a membrane-bound protein that functions to inhibit neuronal migration, adhesion, and neurite outgrowth during development. In the mature nervous system, Nogo-A stabilizes neuronal wiring to inhibit neuronal plasticity and regeneration after injury. Here, we show that RET-1, the sole Nogo-A homolog in Caenorhabditis elegans, is required to control developmental wiring of a specific subset of neurons. In ret-1 deletion mutant animals, specific ventral nerve cord axons are misguided where they fail to respect the ventral midline boundary. We found that ret-1 is expressed in multiple neurons during development, and, through mosaic analysis, showed that ret-1 controls axon guidance in a cell-autonomous manner. Finally, as in mammals, ret-1 regulates ephrin expression, and dysregulation of the ephrin ligand VAB-2 is partially responsible for the ret-1 mutant axonal defects. Together, our data present a previously unidentified function for RET-1 in the nervous system of C. elegans.

Original language	English
Journal	Genetics
Volume	205
Issue number	1
Pages (from-to)	295-302
Number of pages	8
ISSN	0016-6731
DOIs	https://doi.org/10.1534/genetics.115.185322
Publication status	Published - 1 Jan 2017

Keywords

Axon guidance
C. elegans
Ephrin
Nogo-A

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title = "Developmental wiring of specific neurons is regulated by RET-1/Nogo-A in Caenorhabditis elegans",

abstract = "Nogo-A is a membrane-bound protein that functions to inhibit neuronal migration, adhesion, and neurite outgrowth during development. In the mature nervous system, Nogo-A stabilizes neuronal wiring to inhibit neuronal plasticity and regeneration after injury. Here, we show that RET-1, the sole Nogo-A homolog in Caenorhabditis elegans, is required to control developmental wiring of a specific subset of neurons. In ret-1 deletion mutant animals, specific ventral nerve cord axons are misguided where they fail to respect the ventral midline boundary. We found that ret-1 is expressed in multiple neurons during development, and, through mosaic analysis, showed that ret-1 controls axon guidance in a cell-autonomous manner. Finally, as in mammals, ret-1 regulates ephrin expression, and dysregulation of the ephrin ligand VAB-2 is partially responsible for the ret-1 mutant axonal defects. Together, our data present a previously unidentified function for RET-1 in the nervous system of C. elegans.",

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AU - Torpe, Nanna

AU - Nørgaard, Steffen

AU - Høye, Anette M.

AU - Pocock, Roger

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Nogo-A is a membrane-bound protein that functions to inhibit neuronal migration, adhesion, and neurite outgrowth during development. In the mature nervous system, Nogo-A stabilizes neuronal wiring to inhibit neuronal plasticity and regeneration after injury. Here, we show that RET-1, the sole Nogo-A homolog in Caenorhabditis elegans, is required to control developmental wiring of a specific subset of neurons. In ret-1 deletion mutant animals, specific ventral nerve cord axons are misguided where they fail to respect the ventral midline boundary. We found that ret-1 is expressed in multiple neurons during development, and, through mosaic analysis, showed that ret-1 controls axon guidance in a cell-autonomous manner. Finally, as in mammals, ret-1 regulates ephrin expression, and dysregulation of the ephrin ligand VAB-2 is partially responsible for the ret-1 mutant axonal defects. Together, our data present a previously unidentified function for RET-1 in the nervous system of C. elegans.

AB - Nogo-A is a membrane-bound protein that functions to inhibit neuronal migration, adhesion, and neurite outgrowth during development. In the mature nervous system, Nogo-A stabilizes neuronal wiring to inhibit neuronal plasticity and regeneration after injury. Here, we show that RET-1, the sole Nogo-A homolog in Caenorhabditis elegans, is required to control developmental wiring of a specific subset of neurons. In ret-1 deletion mutant animals, specific ventral nerve cord axons are misguided where they fail to respect the ventral midline boundary. We found that ret-1 is expressed in multiple neurons during development, and, through mosaic analysis, showed that ret-1 controls axon guidance in a cell-autonomous manner. Finally, as in mammals, ret-1 regulates ephrin expression, and dysregulation of the ephrin ligand VAB-2 is partially responsible for the ret-1 mutant axonal defects. Together, our data present a previously unidentified function for RET-1 in the nervous system of C. elegans.

KW - Axon guidance

KW - C. elegans

KW - Ephrin

KW - Nogo-A

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DO - 10.1534/genetics.115.185322

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AN - SCOPUS:85008517916

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VL - 205

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EP - 302

JO - Genetics

JF - Genetics

IS - 1

ER -

Developmental wiring of specific neurons is regulated by RET-1/Nogo-A in Caenorhabditis elegans

Abstract

Keywords

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