Development of a rechargeable optical hydrogen peroxide sensor - sensor design and biological application

Klaus Koren; Peter Østrup Jensen; Michael Kühl

doi:10.1039/C6AN00864J

Development of a rechargeable optical hydrogen peroxide sensor - sensor design and biological application

Klaus Koren, Peter Østrup Jensen, Michael Kühl

Marine Biology

19 Citations (Scopus)

63 Downloads (Pure)

Abstract

Hydrogen peroxide (H₂O₂) is an important member of the reactive oxygen species (ROS) family. Among ROS, H₂O₂ is considered the most long-lived and can accumulate inside and outside of cells, where it is involved in both vital (signaling) and deadly (toxic) reactions depending on its concentration. Quantifying H₂O₂ within biological samples is challenging and often not possible. Here we present a quasi-reversible fiber-optic sensor capable of measuring H₂O₂ concentrations ranging from 1-100 μM within different biological samples. Based on a Prussian blue/white redox cycle and a simple sensor recharging and readout strategy, H₂O₂ can be measured with high spatial (∼500 μm) and temporal (∼30 s) resolution. The sensor has a broad applicability both in complex environmental and biomedical systems, as demonstrated by (i) H₂O₂ concentration profile measurements in natural photosynthetic biofilms under light stress reaching H₂O₂ concentrations as high as 15 μM, and (ii) the quantification of the transient increase of the extracellular concentration of H₂O₂ during stimulation of neutrophils.

Original language	English
Journal	Analyst
Volume	141
Issue number	14
Pages (from-to)	4332-4339
Number of pages	8
ISSN	0003-2654
DOIs	https://doi.org/10.1039/C6AN00864J
Publication status	Published - 21 Jul 2016

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title = "Development of a rechargeable optical hydrogen peroxide sensor - sensor design and biological application",

abstract = "Hydrogen peroxide (H2O2) is an important member of the reactive oxygen species (ROS) family. Among ROS, H2O2 is considered the most long-lived and can accumulate inside and outside of cells, where it is involved in both vital (signaling) and deadly (toxic) reactions depending on its concentration. Quantifying H2O2 within biological samples is challenging and often not possible. Here we present a quasi-reversible fiber-optic sensor capable of measuring H2O2 concentrations ranging from 1-100 μM within different biological samples. Based on a Prussian blue/white redox cycle and a simple sensor recharging and readout strategy, H2O2 can be measured with high spatial (∼500 μm) and temporal (∼30 s) resolution. The sensor has a broad applicability both in complex environmental and biomedical systems, as demonstrated by (i) H2O2 concentration profile measurements in natural photosynthetic biofilms under light stress reaching H2O2 concentrations as high as 15 μM, and (ii) the quantification of the transient increase of the extracellular concentration of H2O2 during stimulation of neutrophils.",

author = "Klaus Koren and Jensen, {Peter {\O}strup} and Michael K{\"u}hl",

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T1 - Development of a rechargeable optical hydrogen peroxide sensor - sensor design and biological application

AU - Koren, Klaus

AU - Jensen, Peter Østrup

AU - Kühl, Michael

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Y1 - 2016/7/21

N2 - Hydrogen peroxide (H2O2) is an important member of the reactive oxygen species (ROS) family. Among ROS, H2O2 is considered the most long-lived and can accumulate inside and outside of cells, where it is involved in both vital (signaling) and deadly (toxic) reactions depending on its concentration. Quantifying H2O2 within biological samples is challenging and often not possible. Here we present a quasi-reversible fiber-optic sensor capable of measuring H2O2 concentrations ranging from 1-100 μM within different biological samples. Based on a Prussian blue/white redox cycle and a simple sensor recharging and readout strategy, H2O2 can be measured with high spatial (∼500 μm) and temporal (∼30 s) resolution. The sensor has a broad applicability both in complex environmental and biomedical systems, as demonstrated by (i) H2O2 concentration profile measurements in natural photosynthetic biofilms under light stress reaching H2O2 concentrations as high as 15 μM, and (ii) the quantification of the transient increase of the extracellular concentration of H2O2 during stimulation of neutrophils.

AB - Hydrogen peroxide (H2O2) is an important member of the reactive oxygen species (ROS) family. Among ROS, H2O2 is considered the most long-lived and can accumulate inside and outside of cells, where it is involved in both vital (signaling) and deadly (toxic) reactions depending on its concentration. Quantifying H2O2 within biological samples is challenging and often not possible. Here we present a quasi-reversible fiber-optic sensor capable of measuring H2O2 concentrations ranging from 1-100 μM within different biological samples. Based on a Prussian blue/white redox cycle and a simple sensor recharging and readout strategy, H2O2 can be measured with high spatial (∼500 μm) and temporal (∼30 s) resolution. The sensor has a broad applicability both in complex environmental and biomedical systems, as demonstrated by (i) H2O2 concentration profile measurements in natural photosynthetic biofilms under light stress reaching H2O2 concentrations as high as 15 μM, and (ii) the quantification of the transient increase of the extracellular concentration of H2O2 during stimulation of neutrophils.

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Development of a rechargeable optical hydrogen peroxide sensor - sensor design and biological application

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