TY - JOUR
T1 - Development and evaluation of a multiplex screening assay for Plasmodium falciparum exposure
AU - Jepsen, Micha Phill Grønholm
AU - Röser, Dennis
AU - Christiansen, Michael
AU - Larsen, Severin Olesen
AU - Cavanagh, David R
AU - Dhanasarnsombut, Kelwalin
AU - Bygbjerg, Ib
AU - Dodoo, Daniel
AU - Remarque, Edmond J
AU - Dziegiel, Morten
AU - Jepsen, Søren
AU - Mordmüller, Benjamin
AU - Theisen, Michael
N1 - Copyright © 2012 Elsevier B.V. All rights reserved.
PY - 2012/10/31
Y1 - 2012/10/31
N2 - Transfusion transmitted malaria (TTM) in non-endemic countries is reduced by questioning blood donors and screening of donated blood. Conventional screening is performed by Indirect Fluorescence Antibody Test (IFAT). This method is manual and difficult to standardize. Here we study the diagnostic performance of a multiplex assay for detection of antibodies against Plasmodium falciparum in donor blood using IFAT as a comparator. A multiplex assay (MPA) containing the antigens GLURP-R0, GLURP-R2, MSP3, MSP1 hybrid and AMA1 was constructed using xMAPR technology. A discrimination index for exposure to P. falciparum malaria was calculated by comparing travelers with clinical malaria (n = 52) and non-exposed blood donors (n = 119). The index was evaluated on blood donors with suspected malaria exposure (n = 249) and compared to the diagnostic performance of IFAT.At a specificity of 95.8 %, the MPA discrimination index exhibited a diagnostic sensitivity of 90.4 % in travelers hospitalized with malaria. Percent agreement with IFAT was 92.3 %. Screening plasma from blood donors with suspected malaria exposure, we found 4.8 % to be positive by IFAT and 5.2 % by MPA with an agreement of 93.2 %. The calculated index from the MPA exhibits similar diagnostic performance as IFAT for detection of P. falciparum malaria. Combining the antibody response against multiple antigens in a discrimination index increased the sensitivity of the MPA and reduced the readout to a single value.
AB - Transfusion transmitted malaria (TTM) in non-endemic countries is reduced by questioning blood donors and screening of donated blood. Conventional screening is performed by Indirect Fluorescence Antibody Test (IFAT). This method is manual and difficult to standardize. Here we study the diagnostic performance of a multiplex assay for detection of antibodies against Plasmodium falciparum in donor blood using IFAT as a comparator. A multiplex assay (MPA) containing the antigens GLURP-R0, GLURP-R2, MSP3, MSP1 hybrid and AMA1 was constructed using xMAPR technology. A discrimination index for exposure to P. falciparum malaria was calculated by comparing travelers with clinical malaria (n = 52) and non-exposed blood donors (n = 119). The index was evaluated on blood donors with suspected malaria exposure (n = 249) and compared to the diagnostic performance of IFAT.At a specificity of 95.8 %, the MPA discrimination index exhibited a diagnostic sensitivity of 90.4 % in travelers hospitalized with malaria. Percent agreement with IFAT was 92.3 %. Screening plasma from blood donors with suspected malaria exposure, we found 4.8 % to be positive by IFAT and 5.2 % by MPA with an agreement of 93.2 %. The calculated index from the MPA exhibits similar diagnostic performance as IFAT for detection of P. falciparum malaria. Combining the antibody response against multiple antigens in a discrimination index increased the sensitivity of the MPA and reduced the readout to a single value.
U2 - 10.1016/j.jim.2012.07.009
DO - 10.1016/j.jim.2012.07.009
M3 - Journal article
C2 - 22835432
SN - 0022-1759
VL - 384
SP - 62
EP - 70
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1-2
ER -
