Developing a method for quantification of Ascaris eggs on hands

Aurelie Jeandron; Jeroen J. H.  Ensink; Stig Milan Thamsborg; Anders Dalsgaard; Mita Eva Sengupta

Developing a method for quantification of Ascaris eggs on hands

Aurelie Jeandron, Jeroen J. H. Ensink, Stig Milan Thamsborg, Anders Dalsgaard, Mita Eva Sengupta

Food Safety and Zoonoses

Abstract

In transmission of soil transmitted helminths, especially with Ascaris and Trichuris infections, the importance of hands is unclear and very limited literature exists. This is partly because of the absence of a reliable method to quantify the number of helminth eggs on hands. The aim of this study was to develop a method to assess the number of Ascaris eggs on hands and determine the egg recovery rate of the method. Under laboratory conditions, hands were contaminated with app. 1000 Ascaris eggs, air dried and washed in a plastic bag retaining the washing water, in order to determine recovery rates of eggs for two different detergents (cationic [benzethonium chloride 0.1%], anionic [7X 1% - quadrafos, glycol ether, and dioctyl sulfoccinate sodium salt]) and de-ionized water used as control. The highest recovery rate (95.6%) was achieved with a hand rinse performed with 7X 1%. Washing hands with benzethonium chloride 0.1% and de-ionized water resulted in an egg recovery rate of 88.2 % and 82.7%, respectively. This washing method performed with a low concentration of detergent offers potential for quantitative investigation of contamination of hands with Ascaris eggs and of their role in human infection.

Original language	English
Publication date	2014
Publication status	Published - 2014
Event	ASTMH: 63rd Annual Meeting - Nwe Orleans, United States Duration: 2 Nov 2014 → 6 Nov 2014

Conference

Conference	ASTMH
Country/Territory	United States
City	Nwe Orleans
Period	02/11/2014 → 06/11/2014

Cite this

@conference{102d9b8e7dc44116837e051af218b6dc,

title = "Developing a method for quantification of Ascaris eggs on hands",

abstract = "In transmission of soil transmitted helminths, especially with Ascaris and Trichuris infections, the importance of hands is unclear and very limited literature exists. This is partly because of the absence of a reliable method to quantify the number of helminth eggs on hands. The aim of this study was to develop a method to assess the number of Ascaris eggs on hands and determine the egg recovery rate of the method. Under laboratory conditions, hands were contaminated with app. 1000 Ascaris eggs, air dried and washed in a plastic bag retaining the washing water, in order to determine recovery rates of eggs for two different detergents (cationic [benzethonium chloride 0.1%], anionic [7X 1% - quadrafos, glycol ether, and dioctyl sulfoccinate sodium salt]) and de-ionized water used as control. The highest recovery rate (95.6%) was achieved with a hand rinse performed with 7X 1%. Washing hands with benzethonium chloride 0.1% and de-ionized water resulted in an egg recovery rate of 88.2 % and 82.7%, respectively. This washing method performed with a low concentration of detergent offers potential for quantitative investigation of contamination of hands with Ascaris eggs and of their role in human infection.",

author = "Aurelie Jeandron and Ensink, {Jeroen J. H.} and Thamsborg, {Stig Milan} and Anders Dalsgaard and Sengupta, {Mita Eva}",

year = "2014",

language = "English",

note = "ASTMH ; Conference date: 02-11-2014 Through 06-11-2014",

}

TY - CONF

T1 - Developing a method for quantification of Ascaris eggs on hands

AU - Jeandron, Aurelie

AU - Ensink, Jeroen J. H.

AU - Thamsborg, Stig Milan

AU - Dalsgaard, Anders

AU - Sengupta, Mita Eva

PY - 2014

Y1 - 2014

N2 - In transmission of soil transmitted helminths, especially with Ascaris and Trichuris infections, the importance of hands is unclear and very limited literature exists. This is partly because of the absence of a reliable method to quantify the number of helminth eggs on hands. The aim of this study was to develop a method to assess the number of Ascaris eggs on hands and determine the egg recovery rate of the method. Under laboratory conditions, hands were contaminated with app. 1000 Ascaris eggs, air dried and washed in a plastic bag retaining the washing water, in order to determine recovery rates of eggs for two different detergents (cationic [benzethonium chloride 0.1%], anionic [7X 1% - quadrafos, glycol ether, and dioctyl sulfoccinate sodium salt]) and de-ionized water used as control. The highest recovery rate (95.6%) was achieved with a hand rinse performed with 7X 1%. Washing hands with benzethonium chloride 0.1% and de-ionized water resulted in an egg recovery rate of 88.2 % and 82.7%, respectively. This washing method performed with a low concentration of detergent offers potential for quantitative investigation of contamination of hands with Ascaris eggs and of their role in human infection.

AB - In transmission of soil transmitted helminths, especially with Ascaris and Trichuris infections, the importance of hands is unclear and very limited literature exists. This is partly because of the absence of a reliable method to quantify the number of helminth eggs on hands. The aim of this study was to develop a method to assess the number of Ascaris eggs on hands and determine the egg recovery rate of the method. Under laboratory conditions, hands were contaminated with app. 1000 Ascaris eggs, air dried and washed in a plastic bag retaining the washing water, in order to determine recovery rates of eggs for two different detergents (cationic [benzethonium chloride 0.1%], anionic [7X 1% - quadrafos, glycol ether, and dioctyl sulfoccinate sodium salt]) and de-ionized water used as control. The highest recovery rate (95.6%) was achieved with a hand rinse performed with 7X 1%. Washing hands with benzethonium chloride 0.1% and de-ionized water resulted in an egg recovery rate of 88.2 % and 82.7%, respectively. This washing method performed with a low concentration of detergent offers potential for quantitative investigation of contamination of hands with Ascaris eggs and of their role in human infection.

M3 - Poster

T2 - ASTMH

Y2 - 2 November 2014 through 6 November 2014

ER -