Determination of the Wound Healing Potentials of Medicinal Plants Historically Used in Ghana

Sara Holm Freiesleben; Jens Soelberg; Nils Nyberg; Anna K Jäger

doi:10.1155/2017/9480791

Determination of the Wound Healing Potentials of Medicinal Plants Historically Used in Ghana

Sara Holm Freiesleben, Jens Soelberg, Nils Nyberg, Anna K Jäger

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Abstract

The present study was carried out to investigate the wound healing potentials of 17 medicinal plants historically used in Ghana for wound healing. Warm and cold water extracts were prepared from the 17 dried plant species and tested in vitro in the scratch assay with NIH 3T3 fibroblasts from mice.The wound healing scratch assay was used to evaluate the effect of the plants on cell proliferation and/or migration in vitro, as a test for potential wound healing properties. After 21 hours of incubation increased proliferation and/or migration of fibroblasts in the scratch assay was obtained for 5 out of the 17 plant species. HPLC separation of the most active plant extract, which was a warm water extract of Philenoptera cyanescens, revealed the wound healing activity to be attributed to rutin and a triglycoside of quercetin. The present study suggests that Allophylus spicatus, Philenoptera cyanescens, Melanthera scandens, Ocimum gratissimum, and Jasminum dichotomum have wound healing activity in vitro.

Original language	English
Article number	9480791
Journal	Evidence - Based Complementary and Alternative Medicine
Volume	2017
Number of pages	6
ISSN	1741-427X
DOIs	https://doi.org/10.1155/2017/9480791
Publication status	Published - 2017

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10.1155/2017/9480791Licence: CC BY

9480791Licence: CC BY

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title = "Determination of the Wound Healing Potentials of Medicinal Plants Historically Used in Ghana",

abstract = "The present study was carried out to investigate the wound healing potentials of 17 medicinal plants historically used in Ghana for wound healing. Warm and cold water extracts were prepared from the 17 dried plant species and tested in vitro in the scratch assay with NIH 3T3 fibroblasts from mice.The wound healing scratch assay was used to evaluate the effect of the plants on cell proliferation and/or migration in vitro, as a test for potential wound healing properties. After 21 hours of incubation increased proliferation and/or migration of fibroblasts in the scratch assay was obtained for 5 out of the 17 plant species. HPLC separation of the most active plant extract, which was a warm water extract of Philenoptera cyanescens, revealed the wound healing activity to be attributed to rutin and a triglycoside of quercetin. The present study suggests that Allophylus spicatus, Philenoptera cyanescens, Melanthera scandens, Ocimum gratissimum, and Jasminum dichotomum have wound healing activity in vitro.",

author = "Freiesleben, {Sara Holm} and Jens Soelberg and Nils Nyberg and J{\"a}ger, {Anna K}",

year = "2017",

doi = "10.1155/2017/9480791",

language = "English",

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journal = "Evidence-based Complementary and Alternative Medicine",

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T1 - Determination of the Wound Healing Potentials of Medicinal Plants Historically Used in Ghana

AU - Freiesleben, Sara Holm

AU - Soelberg, Jens

AU - Nyberg, Nils

AU - Jäger, Anna K

PY - 2017

Y1 - 2017

N2 - The present study was carried out to investigate the wound healing potentials of 17 medicinal plants historically used in Ghana for wound healing. Warm and cold water extracts were prepared from the 17 dried plant species and tested in vitro in the scratch assay with NIH 3T3 fibroblasts from mice.The wound healing scratch assay was used to evaluate the effect of the plants on cell proliferation and/or migration in vitro, as a test for potential wound healing properties. After 21 hours of incubation increased proliferation and/or migration of fibroblasts in the scratch assay was obtained for 5 out of the 17 plant species. HPLC separation of the most active plant extract, which was a warm water extract of Philenoptera cyanescens, revealed the wound healing activity to be attributed to rutin and a triglycoside of quercetin. The present study suggests that Allophylus spicatus, Philenoptera cyanescens, Melanthera scandens, Ocimum gratissimum, and Jasminum dichotomum have wound healing activity in vitro.

AB - The present study was carried out to investigate the wound healing potentials of 17 medicinal plants historically used in Ghana for wound healing. Warm and cold water extracts were prepared from the 17 dried plant species and tested in vitro in the scratch assay with NIH 3T3 fibroblasts from mice.The wound healing scratch assay was used to evaluate the effect of the plants on cell proliferation and/or migration in vitro, as a test for potential wound healing properties. After 21 hours of incubation increased proliferation and/or migration of fibroblasts in the scratch assay was obtained for 5 out of the 17 plant species. HPLC separation of the most active plant extract, which was a warm water extract of Philenoptera cyanescens, revealed the wound healing activity to be attributed to rutin and a triglycoside of quercetin. The present study suggests that Allophylus spicatus, Philenoptera cyanescens, Melanthera scandens, Ocimum gratissimum, and Jasminum dichotomum have wound healing activity in vitro.

U2 - 10.1155/2017/9480791

DO - 10.1155/2017/9480791

M3 - Journal article

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SN - 1741-427X

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JO - Evidence-based Complementary and Alternative Medicine

JF - Evidence-based Complementary and Alternative Medicine

M1 - 9480791

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Determination of the Wound Healing Potentials of Medicinal Plants Historically Used in Ghana

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