Determination of steroid hormones in blood by GC-MS/MS

Martin Hansen; Naja Wessel Jacobsen; Frederik Knud Nielsen; Erland Björklund; Bjarne Styrishave; Bent Halling-Sørensen

doi:10.1007/s00216-011-5038-8

Determination of steroid hormones in blood by GC-MS/MS

Martin Hansen, Naja Wessel Jacobsen, Frederik Knud Nielsen, Erland Björklund, Bjarne Styrishave, Bent Halling-Sørensen

73 Citations (Scopus)

Abstract

This paper presents the development, optimization and validation of a methodology to determine nine key steroid hormones (viz. pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone, dihydrotestosterone, estrone, 17α-estradiol and 17β-estradiol) expressed in the steroidogenesis in biological fluids. The analytical method allows for the determination of steroid hormones in blood plasma and serum down to 0.08-0.16 ng/mL for estrogens, 0.20-0.36 ng/mL for androgens and 0.36-0.43 ng/mL for progestagens. These limits of detection were obtainable using a two-step solid-phase clean-up for fractionation and elimination of interfering lipids (fatty acids, phospholipids, glycerides and sterols) from the steroid hormones. The accuracy of the method was 50-112% in the range 0.10 to 2.00 ng/mL. [Figure not available: see fulltext.]

Original language	English
Journal	Analytical and Bioanalytical Chemistry
Volume	400
Pages (from-to)	3409-3417
ISSN	1618-2642
DOIs	https://doi.org/10.1007/s00216-011-5038-8
Publication status	Published - Jul 2011

Keywords

Former Faculty of Pharmaceutical Sciences

Access to Document

10.1007/s00216-011-5038-8

Cite this

@article{4a95cf1e83b14b8da53816998daae399,

title = "Determination of steroid hormones in blood by GC-MS/MS",

abstract = "This paper presents the development, optimization and validation of a methodology to determine nine key steroid hormones (viz. pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone, dihydrotestosterone, estrone, 17α-estradiol and 17β-estradiol) expressed in the steroidogenesis in biological fluids. The analytical method allows for the determination of steroid hormones in blood plasma and serum down to 0.08-0.16 ng/mL for estrogens, 0.20-0.36 ng/mL for androgens and 0.36-0.43 ng/mL for progestagens. These limits of detection were obtainable using a two-step solid-phase clean-up for fractionation and elimination of interfering lipids (fatty acids, phospholipids, glycerides and sterols) from the steroid hormones. The accuracy of the method was 50-112% in the range 0.10 to 2.00 ng/mL. [Figure not available: see fulltext.]",

keywords = "Former Faculty of Pharmaceutical Sciences",

author = "Martin Hansen and Jacobsen, {Naja Wessel} and Nielsen, {Frederik Knud} and Erland Bj{\"o}rklund and Bjarne Styrishave and Bent Halling-S{\o}rensen",

year = "2011",

month = jul,

doi = "10.1007/s00216-011-5038-8",

language = "English",

volume = "400",

pages = "3409--3417",

journal = "Analytical and Bioanalytical Chemistry",

issn = "1618-2642",

publisher = "Springer",

}

TY - JOUR

T1 - Determination of steroid hormones in blood by GC-MS/MS

AU - Hansen, Martin

AU - Jacobsen, Naja Wessel

AU - Nielsen, Frederik Knud

AU - Björklund, Erland

AU - Styrishave, Bjarne

AU - Halling-Sørensen, Bent

PY - 2011/7

Y1 - 2011/7

N2 - This paper presents the development, optimization and validation of a methodology to determine nine key steroid hormones (viz. pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone, dihydrotestosterone, estrone, 17α-estradiol and 17β-estradiol) expressed in the steroidogenesis in biological fluids. The analytical method allows for the determination of steroid hormones in blood plasma and serum down to 0.08-0.16 ng/mL for estrogens, 0.20-0.36 ng/mL for androgens and 0.36-0.43 ng/mL for progestagens. These limits of detection were obtainable using a two-step solid-phase clean-up for fractionation and elimination of interfering lipids (fatty acids, phospholipids, glycerides and sterols) from the steroid hormones. The accuracy of the method was 50-112% in the range 0.10 to 2.00 ng/mL. [Figure not available: see fulltext.]

AB - This paper presents the development, optimization and validation of a methodology to determine nine key steroid hormones (viz. pregnenolone, progesterone, dehydroepiandrosterone, androstenedione, testosterone, dihydrotestosterone, estrone, 17α-estradiol and 17β-estradiol) expressed in the steroidogenesis in biological fluids. The analytical method allows for the determination of steroid hormones in blood plasma and serum down to 0.08-0.16 ng/mL for estrogens, 0.20-0.36 ng/mL for androgens and 0.36-0.43 ng/mL for progestagens. These limits of detection were obtainable using a two-step solid-phase clean-up for fractionation and elimination of interfering lipids (fatty acids, phospholipids, glycerides and sterols) from the steroid hormones. The accuracy of the method was 50-112% in the range 0.10 to 2.00 ng/mL. [Figure not available: see fulltext.]

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1007/s00216-011-5038-8

DO - 10.1007/s00216-011-5038-8

M3 - Journal article

C2 - 21533798

SN - 1618-2642

VL - 400

SP - 3409

EP - 3417

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

ER -

Determination of steroid hormones in blood by GC-MS/MS

Abstract

Keywords

Access to Document

Fingerprint

Cite this