Determination of psilocybin in Psilocybe semilanceata by capillary zone electrophoresis

Stig Pedersen-Bjergaard; Ellen Sannes; Knut Einar Rasmussen; Finn Tønnesen

doi:10.1016/S0378-4347(97)00127-8

Determination of psilocybin in Psilocybe semilanceata by capillary zone electrophoresis

Stig Pedersen-Bjergaard^*, Ellen Sannes, Knut Einar Rasmussen, Finn Tønnesen

^*Corresponding author for this work

19 Citations (Scopus)

Abstract

A capillary zone electrophoretic (CZE) method was developed for the rapid determination of psilocybin in Psilocybe semilanceata. Following a simple two step extraction with 3.0+2.0 ml methanol, the hallucinogenic compound was effectively separated from matrix components by CZE utilizing a 10 mM borate-phosphate running buffer adjusted to pH 11.5. The identity of psilocybin was confirmed by migration time information and by W spectra, while quantitation was accomplished utilizing barbital as internal standard. The calibration curve for psilocybin was linear within 0.01-1 mg/ml, while intra-day and inter-day variations of quantitative data were 0.5 and 2.5% R.S.D., respectively. In addition to psilocybin, the method was also suitable for the determination of the structurally related compound baeocystin.

Original language	English
Journal	Journal of Chromatography B: Biomedical Applications
Volume	694
Issue number	2
Pages (from-to)	375-381
Number of pages	7
ISSN	1572-6495
DOIs	https://doi.org/10.1016/S0378-4347(97)00127-8
Publication status	Published - 4 Jul 1997

Keywords

Psilocybin

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Pedersen-Bjergaard, S., Sannes, E., Rasmussen, K. E., & Tønnesen, F. (1997). Determination of psilocybin in Psilocybe semilanceata by capillary zone electrophoresis. Journal of Chromatography B: Biomedical Applications, 694(2), 375-381. https://doi.org/10.1016/S0378-4347(97)00127-8

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abstract = "A capillary zone electrophoretic (CZE) method was developed for the rapid determination of psilocybin in Psilocybe semilanceata. Following a simple two step extraction with 3.0+2.0 ml methanol, the hallucinogenic compound was effectively separated from matrix components by CZE utilizing a 10 mM borate-phosphate running buffer adjusted to pH 11.5. The identity of psilocybin was confirmed by migration time information and by W spectra, while quantitation was accomplished utilizing barbital as internal standard. The calibration curve for psilocybin was linear within 0.01-1 mg/ml, while intra-day and inter-day variations of quantitative data were 0.5 and 2.5% R.S.D., respectively. In addition to psilocybin, the method was also suitable for the determination of the structurally related compound baeocystin.",

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AU - Pedersen-Bjergaard, Stig

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AU - Rasmussen, Knut Einar

AU - Tønnesen, Finn

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N2 - A capillary zone electrophoretic (CZE) method was developed for the rapid determination of psilocybin in Psilocybe semilanceata. Following a simple two step extraction with 3.0+2.0 ml methanol, the hallucinogenic compound was effectively separated from matrix components by CZE utilizing a 10 mM borate-phosphate running buffer adjusted to pH 11.5. The identity of psilocybin was confirmed by migration time information and by W spectra, while quantitation was accomplished utilizing barbital as internal standard. The calibration curve for psilocybin was linear within 0.01-1 mg/ml, while intra-day and inter-day variations of quantitative data were 0.5 and 2.5% R.S.D., respectively. In addition to psilocybin, the method was also suitable for the determination of the structurally related compound baeocystin.

AB - A capillary zone electrophoretic (CZE) method was developed for the rapid determination of psilocybin in Psilocybe semilanceata. Following a simple two step extraction with 3.0+2.0 ml methanol, the hallucinogenic compound was effectively separated from matrix components by CZE utilizing a 10 mM borate-phosphate running buffer adjusted to pH 11.5. The identity of psilocybin was confirmed by migration time information and by W spectra, while quantitation was accomplished utilizing barbital as internal standard. The calibration curve for psilocybin was linear within 0.01-1 mg/ml, while intra-day and inter-day variations of quantitative data were 0.5 and 2.5% R.S.D., respectively. In addition to psilocybin, the method was also suitable for the determination of the structurally related compound baeocystin.

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Determination of psilocybin in Psilocybe semilanceata by capillary zone electrophoresis

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