Determination of ketone bodies in blood by headspace gas chromatography-mass spectrometry

Karen Marie Dollerup Holm; Kristian Linnet; Brian Schou Rasmussen; Anders Just Pedersen

Determination of ketone bodies in blood by headspace gas chromatography-mass spectrometry

Karen Marie Dollerup Holm, Kristian Linnet, Brian Schou Rasmussen, Anders Just Pedersen

Section of Forensic Chemistry

4 Citations (Scopus)

Abstract

A gas chromatography-mass spectrometry (GC-MS) method for determination of ketone bodies (β-hydroxybutyrate, acetone, and acetoacetate) in blood is presented. The method is based on enzymatic oxidation of D-β-hydroxybutyrate to acetoacetate, followed by decarboxylation to acetone, which was quantified by the use of headspace GC-MS using acetone-13C₃ as an internal standard. The developed method was found to have intra- and total interday relative standard deviations < 10% for acetone+acetoacetate levels (~25 to 8300 μM) and D-β-hydroxybutyrate levels (~30 to 16500 μM). Recovery values varied from 98 to 107%, demonstrating the suitability of the method for measuring ketone bodies over a wide concentration range. The method has been applied to cases in which ketoacidosis was suspected as the cause of death in diabetics or chronic alcoholics, as well as to cases in which another cause of death was identified.

Original language	English
Journal	Journal of Analytical Toxicology
Volume	34
Issue number	9
Pages (from-to)	549-54
Number of pages	6
ISSN	0146-4760
Publication status	Published - Nov 2010

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title = "Determination of ketone bodies in blood by headspace gas chromatography-mass spectrometry",

abstract = "A gas chromatography-mass spectrometry (GC-MS) method for determination of ketone bodies (β-hydroxybutyrate, acetone, and acetoacetate) in blood is presented. The method is based on enzymatic oxidation of D-β-hydroxybutyrate to acetoacetate, followed by decarboxylation to acetone, which was quantified by the use of headspace GC-MS using acetone-13C3 as an internal standard. The developed method was found to have intra- and total interday relative standard deviations < 10% for acetone+acetoacetate levels (~25 to 8300 μM) and D-β-hydroxybutyrate levels (~30 to 16500 μM). Recovery values varied from 98 to 107%, demonstrating the suitability of the method for measuring ketone bodies over a wide concentration range. The method has been applied to cases in which ketoacidosis was suspected as the cause of death in diabetics or chronic alcoholics, as well as to cases in which another cause of death was identified.",

author = "Holm, {Karen Marie Dollerup} and Kristian Linnet and Rasmussen, {Brian Schou} and Pedersen, {Anders Just}",

year = "2010",

month = nov,

language = "English",

volume = "34",

pages = "549--54",

journal = "Journal of Analytical Toxicology",

issn = "0146-4760",

publisher = "Oxford University Press",

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TY - JOUR

T1 - Determination of ketone bodies in blood by headspace gas chromatography-mass spectrometry

AU - Holm, Karen Marie Dollerup

AU - Linnet, Kristian

AU - Rasmussen, Brian Schou

AU - Pedersen, Anders Just

PY - 2010/11

Y1 - 2010/11

N2 - A gas chromatography-mass spectrometry (GC-MS) method for determination of ketone bodies (β-hydroxybutyrate, acetone, and acetoacetate) in blood is presented. The method is based on enzymatic oxidation of D-β-hydroxybutyrate to acetoacetate, followed by decarboxylation to acetone, which was quantified by the use of headspace GC-MS using acetone-13C3 as an internal standard. The developed method was found to have intra- and total interday relative standard deviations < 10% for acetone+acetoacetate levels (~25 to 8300 μM) and D-β-hydroxybutyrate levels (~30 to 16500 μM). Recovery values varied from 98 to 107%, demonstrating the suitability of the method for measuring ketone bodies over a wide concentration range. The method has been applied to cases in which ketoacidosis was suspected as the cause of death in diabetics or chronic alcoholics, as well as to cases in which another cause of death was identified.

AB - A gas chromatography-mass spectrometry (GC-MS) method for determination of ketone bodies (β-hydroxybutyrate, acetone, and acetoacetate) in blood is presented. The method is based on enzymatic oxidation of D-β-hydroxybutyrate to acetoacetate, followed by decarboxylation to acetone, which was quantified by the use of headspace GC-MS using acetone-13C3 as an internal standard. The developed method was found to have intra- and total interday relative standard deviations < 10% for acetone+acetoacetate levels (~25 to 8300 μM) and D-β-hydroxybutyrate levels (~30 to 16500 μM). Recovery values varied from 98 to 107%, demonstrating the suitability of the method for measuring ketone bodies over a wide concentration range. The method has been applied to cases in which ketoacidosis was suspected as the cause of death in diabetics or chronic alcoholics, as well as to cases in which another cause of death was identified.

M3 - Journal article

C2 - 21073807

SN - 0146-4760

VL - 34

SP - 549

EP - 554

JO - Journal of Analytical Toxicology

JF - Journal of Analytical Toxicology

IS - 9

ER -

Determination of ketone bodies in blood by headspace gas chromatography-mass spectrometry

Abstract

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