Detection of Ultrafine Anaphase Bridges

Anna H Bizard; Christian F Nielsen; Ian D Hickson

doi:10.1007/978-1-4939-7306-4_33

Detection of Ultrafine Anaphase Bridges

Anna H Bizard, Christian F Nielsen, Ian D Hickson

8 Citations (Scopus)

Abstract

Ultrafine anaphase bridges (UFBs) are thin DNA threads linking the separating sister chromatids in the anaphase of mitosis. UFBs are thought to form when topological DNA entanglements between two chromatids are not resolved prior to anaphase onset. In contrast to other markers of defective chromosome segregation, UFBs cannot be detected by direct staining of the DNA, but instead can be detected using immunofluorescence-based approaches. Due to the fact that they are short-lived and fragile in nature, UFBs can be challenging to detect. In this chapter, we describe methods that have been optimized for successful detection of UFBs. We also provide guidelines for the optimization of UFBs detection depending on the antibody and the cell line to be used.

Original language	English
Title of host publication	Genome Instability : Methods and Protocols
Number of pages	14
Volume	1672
Publisher	Springer
Publication date	2018
Pages	495-508
ISBN (Print)	978-1-4939-7305-7
ISBN (Electronic)	978-1-4939-7306-4
DOIs	https://doi.org/10.1007/978-1-4939-7306-4_33
Publication status	Published - 2018

Series	Methods in molecular biology (Clifton, N.J.)
ISSN	1064-3745

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10.1007/978-1-4939-7306-4_33

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AU - Bizard, Anna H

AU - Nielsen, Christian F

AU - Hickson, Ian D

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N2 - Ultrafine anaphase bridges (UFBs) are thin DNA threads linking the separating sister chromatids in the anaphase of mitosis. UFBs are thought to form when topological DNA entanglements between two chromatids are not resolved prior to anaphase onset. In contrast to other markers of defective chromosome segregation, UFBs cannot be detected by direct staining of the DNA, but instead can be detected using immunofluorescence-based approaches. Due to the fact that they are short-lived and fragile in nature, UFBs can be challenging to detect. In this chapter, we describe methods that have been optimized for successful detection of UFBs. We also provide guidelines for the optimization of UFBs detection depending on the antibody and the cell line to be used.

AB - Ultrafine anaphase bridges (UFBs) are thin DNA threads linking the separating sister chromatids in the anaphase of mitosis. UFBs are thought to form when topological DNA entanglements between two chromatids are not resolved prior to anaphase onset. In contrast to other markers of defective chromosome segregation, UFBs cannot be detected by direct staining of the DNA, but instead can be detected using immunofluorescence-based approaches. Due to the fact that they are short-lived and fragile in nature, UFBs can be challenging to detect. In this chapter, we describe methods that have been optimized for successful detection of UFBs. We also provide guidelines for the optimization of UFBs detection depending on the antibody and the cell line to be used.

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Detection of Ultrafine Anaphase Bridges

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