Detection of Pathogenic Biofilms with Bacterial Amyloid Targeting Fluorescent Probe, CDy11

Jun Young Kim; Srikanta Sahu; Yin Hoe Yau; Xu Wang; Susana Geifman Shochat; Per Halkjær Nielsen; Morten Simonsen Dueholm; Daniel E. Otzen; Jungyeol Lee; May Margarette Salido Delos Santos; Joey Kuok Hoong Yam; Nam Young Kang; Sung Jin Park; Hawyoung Kwon; Thomas Seviour; Liang Yang; Michael Givskov; Young Tae Chang

doi:10.1021/jacs.5b11357

Detection of Pathogenic Biofilms with Bacterial Amyloid Targeting Fluorescent Probe, CDy11

Jun Young Kim, Srikanta Sahu, Yin Hoe Yau, Xu Wang, Susana Geifman Shochat, Per Halkjær Nielsen, Morten Simonsen Dueholm, Daniel E. Otzen, Jungyeol Lee, May Margarette Salido Delos Santos, Joey Kuok Hoong Yam, Nam Young Kang, Sung Jin Park, Hawyoung Kwon, Thomas Seviour, Liang Yang, Michael Givskov, Young Tae Chang^*

^*Corresponding author for this work

48 Citations (Scopus)

Abstract

Bacterial biofilms are responsible for a wide range of persistent infections. In the clinic, diagnosis of biofilm-associated infections relies heavily on culturing methods, which fail to detect nonculturable bacteria. Identification of novel fluorescent probes for biofilm imaging will greatly facilitate diagnosis of pathogenic bacterial infection. Herein, we report a novel fluorescent probe, CDy11 (compound of designation yellow 11), which targets amyloid in the Pseudomonas aeruginosa biofilm matrix through a diversity oriented fluorescent library approach (DOFLA). CDy11 was further demonstrated for in vivo imaging of P. aeruginosa in implant and corneal infection mice models.

Original language	English
Journal	Journal of the American Chemical Society
Volume	138
Issue number	1
Pages (from-to)	402-407
Number of pages	6
ISSN	0002-7863
DOIs	https://doi.org/10.1021/jacs.5b11357
Publication status	Published - 13 Jan 2016

Access to Document

10.1021/jacs.5b11357

Cite this

Kim, J. Y., Sahu, S., Yau, Y. H., Wang, X., Shochat, S. G., Nielsen, P. H., Dueholm, M. S., Otzen, D. E., Lee, J., Delos Santos, M. M. S., Yam, J. K. H., Kang, N. Y., Park, S. J., Kwon, H., Seviour, T., Yang, L., Givskov, M., & Chang, Y. T. (2016). Detection of Pathogenic Biofilms with Bacterial Amyloid Targeting Fluorescent Probe, CDy11. Journal of the American Chemical Society, 138(1), 402-407. https://doi.org/10.1021/jacs.5b11357

Kim, JY, Sahu, S, Yau, YH, Wang, X, Shochat, SG, Nielsen, PH, Dueholm, MS, Otzen, DE, Lee, J, Delos Santos, MMS, Yam, JKH, Kang, NY, Park, SJ, Kwon, H, Seviour, T, Yang, L, Givskov, M & Chang, YT 2016, 'Detection of Pathogenic Biofilms with Bacterial Amyloid Targeting Fluorescent Probe, CDy11', Journal of the American Chemical Society, vol. 138, no. 1, pp. 402-407. https://doi.org/10.1021/jacs.5b11357

@article{4e80994c808046258c457c07a95cb69f,

title = "Detection of Pathogenic Biofilms with Bacterial Amyloid Targeting Fluorescent Probe, CDy11",

abstract = "Bacterial biofilms are responsible for a wide range of persistent infections. In the clinic, diagnosis of biofilm-associated infections relies heavily on culturing methods, which fail to detect nonculturable bacteria. Identification of novel fluorescent probes for biofilm imaging will greatly facilitate diagnosis of pathogenic bacterial infection. Herein, we report a novel fluorescent probe, CDy11 (compound of designation yellow 11), which targets amyloid in the Pseudomonas aeruginosa biofilm matrix through a diversity oriented fluorescent library approach (DOFLA). CDy11 was further demonstrated for in vivo imaging of P. aeruginosa in implant and corneal infection mice models.",

author = "Kim, {Jun Young} and Srikanta Sahu and Yau, {Yin Hoe} and Xu Wang and Shochat, {Susana Geifman} and Nielsen, {Per Halkj{\ae}r} and Dueholm, {Morten Simonsen} and Otzen, {Daniel E.} and Jungyeol Lee and {Delos Santos}, {May Margarette Salido} and Yam, {Joey Kuok Hoong} and Kang, {Nam Young} and Park, {Sung Jin} and Hawyoung Kwon and Thomas Seviour and Liang Yang and Michael Givskov and Chang, {Young Tae}",

year = "2016",

month = jan,

day = "13",

doi = "10.1021/jacs.5b11357",

language = "English",

volume = "138",

pages = "402--407",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "ACS Publications",

number = "1",

}

TY - JOUR

T1 - Detection of Pathogenic Biofilms with Bacterial Amyloid Targeting Fluorescent Probe, CDy11

AU - Kim, Jun Young

AU - Sahu, Srikanta

AU - Yau, Yin Hoe

AU - Wang, Xu

AU - Shochat, Susana Geifman

AU - Nielsen, Per Halkjær

AU - Dueholm, Morten Simonsen

AU - Otzen, Daniel E.

AU - Lee, Jungyeol

AU - Delos Santos, May Margarette Salido

AU - Yam, Joey Kuok Hoong

AU - Kang, Nam Young

AU - Park, Sung Jin

AU - Kwon, Hawyoung

AU - Seviour, Thomas

AU - Yang, Liang

AU - Givskov, Michael

AU - Chang, Young Tae

PY - 2016/1/13

Y1 - 2016/1/13

N2 - Bacterial biofilms are responsible for a wide range of persistent infections. In the clinic, diagnosis of biofilm-associated infections relies heavily on culturing methods, which fail to detect nonculturable bacteria. Identification of novel fluorescent probes for biofilm imaging will greatly facilitate diagnosis of pathogenic bacterial infection. Herein, we report a novel fluorescent probe, CDy11 (compound of designation yellow 11), which targets amyloid in the Pseudomonas aeruginosa biofilm matrix through a diversity oriented fluorescent library approach (DOFLA). CDy11 was further demonstrated for in vivo imaging of P. aeruginosa in implant and corneal infection mice models.

AB - Bacterial biofilms are responsible for a wide range of persistent infections. In the clinic, diagnosis of biofilm-associated infections relies heavily on culturing methods, which fail to detect nonculturable bacteria. Identification of novel fluorescent probes for biofilm imaging will greatly facilitate diagnosis of pathogenic bacterial infection. Herein, we report a novel fluorescent probe, CDy11 (compound of designation yellow 11), which targets amyloid in the Pseudomonas aeruginosa biofilm matrix through a diversity oriented fluorescent library approach (DOFLA). CDy11 was further demonstrated for in vivo imaging of P. aeruginosa in implant and corneal infection mice models.

U2 - 10.1021/jacs.5b11357

DO - 10.1021/jacs.5b11357

M3 - Journal article

C2 - 26684612

AN - SCOPUS:84954342145

SN - 0002-7863

VL - 138

SP - 402

EP - 407

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 1

ER -

Detection of Pathogenic Biofilms with Bacterial Amyloid Targeting Fluorescent Probe, CDy11

Abstract

Access to Document

Fingerprint

Cite this