Detection of autoantibodies to cytokines

K Bendtzen; M B Hansen; C Ross; M Svenson

doi:10.1385/MB:14:3:251

Detection of autoantibodies to cytokines

K Bendtzen, M B Hansen, C Ross, M Svenson

Department of Clinical Medicine

33 Citations (Scopus)

Abstract

Autoantibodies to various cytokines have been reported in normal individuals and in patients with various infectious and immunoinflammatory disorders, and similar antibodies (Ab) may be induced in patients receiving human recombinant cytokines. The clinical relevance of these Ab is often difficult to evaluate. Not only are in vitro neutralizing cytokine Ab not necessarily neutralizing in vivo, but assays for binding and neutralizing Ab to cytokines are often difficult to interpret. For example, denaturation of immobilized cytokines in immunoblotting techniques and immunometric assays may leave Ab to the native forms of the mediators unrecognized. On the other hand, Ab may bind nonspecifically and/or with biologically irrelevant low affinities, leading to erroneous interpretations. This article describes in detail the use of radioimmunoassays that we have optimized and used successfully for the detection of high-affinity (auto) Ab to IL-1 alpha, IL-6, GM-CSF, and IFN alpha.

Original language	English
Journal	Molecular Biotechnology
Volume	14
Issue number	3
Pages (from-to)	251-61
Number of pages	11
ISSN	1073-6085
DOIs	https://doi.org/10.1385/MB:14:3:251
Publication status	Published - 1 Mar 2000

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title = "Detection of autoantibodies to cytokines",

abstract = "Autoantibodies to various cytokines have been reported in normal individuals and in patients with various infectious and immunoinflammatory disorders, and similar antibodies (Ab) may be induced in patients receiving human recombinant cytokines. The clinical relevance of these Ab is often difficult to evaluate. Not only are in vitro neutralizing cytokine Ab not necessarily neutralizing in vivo, but assays for binding and neutralizing Ab to cytokines are often difficult to interpret. For example, denaturation of immobilized cytokines in immunoblotting techniques and immunometric assays may leave Ab to the native forms of the mediators unrecognized. On the other hand, Ab may bind nonspecifically and/or with biologically irrelevant low affinities, leading to erroneous interpretations. This article describes in detail the use of radioimmunoassays that we have optimized and used successfully for the detection of high-affinity (auto) Ab to IL-1 alpha, IL-6, GM-CSF, and IFN alpha.",

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T1 - Detection of autoantibodies to cytokines

AU - Bendtzen, K

AU - Hansen, M B

AU - Ross, C

AU - Svenson, M

PY - 2000/3/1

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N2 - Autoantibodies to various cytokines have been reported in normal individuals and in patients with various infectious and immunoinflammatory disorders, and similar antibodies (Ab) may be induced in patients receiving human recombinant cytokines. The clinical relevance of these Ab is often difficult to evaluate. Not only are in vitro neutralizing cytokine Ab not necessarily neutralizing in vivo, but assays for binding and neutralizing Ab to cytokines are often difficult to interpret. For example, denaturation of immobilized cytokines in immunoblotting techniques and immunometric assays may leave Ab to the native forms of the mediators unrecognized. On the other hand, Ab may bind nonspecifically and/or with biologically irrelevant low affinities, leading to erroneous interpretations. This article describes in detail the use of radioimmunoassays that we have optimized and used successfully for the detection of high-affinity (auto) Ab to IL-1 alpha, IL-6, GM-CSF, and IFN alpha.

AB - Autoantibodies to various cytokines have been reported in normal individuals and in patients with various infectious and immunoinflammatory disorders, and similar antibodies (Ab) may be induced in patients receiving human recombinant cytokines. The clinical relevance of these Ab is often difficult to evaluate. Not only are in vitro neutralizing cytokine Ab not necessarily neutralizing in vivo, but assays for binding and neutralizing Ab to cytokines are often difficult to interpret. For example, denaturation of immobilized cytokines in immunoblotting techniques and immunometric assays may leave Ab to the native forms of the mediators unrecognized. On the other hand, Ab may bind nonspecifically and/or with biologically irrelevant low affinities, leading to erroneous interpretations. This article describes in detail the use of radioimmunoassays that we have optimized and used successfully for the detection of high-affinity (auto) Ab to IL-1 alpha, IL-6, GM-CSF, and IFN alpha.

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DO - 10.1385/MB:14:3:251

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VL - 14

SP - 251

EP - 261

JO - Molecular Biotechnology

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Detection of autoantibodies to cytokines

Abstract

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