Detection of a quantitative trait locus associated with resistance to Ascaris suum infection in pigs

Per Skallerup; Peter Nejsum; Claus Bøttcher Jørgensen; Harald H. H. Göring; Peter Karlskov-Mortensen; Alan L.  Archibald; Merete Fredholm; Stig Milan Thamsborg

doi:10.1016/j.ijpara.2012.02.010

Detection of a quantitative trait locus associated with resistance to Ascaris suum infection in pigs

Per Skallerup, Peter Nejsum, Claus Bøttcher Jørgensen, Harald H. H. Göring, Peter Karlskov-Mortensen, Alan L. Archibald, Merete Fredholm, Stig Milan Thamsborg

12 Citations (Scopus)

Abstract

Helminths almost invariably have an over-dispersed distribution in the host population. Human and animal studies have provided evidence suggesting that a large part of this variation is due to host genetic factors. Recently, the heritability for roundworm (. Ascaris suum) infection levels in pigs was estimated to be 0.45. We used single nucleotide polymorphism markers to perform a whole-genome scan on 195 pigs experimentally infected with . A. suum. A putative quantitative trait locus for worm burden on chromosome 4 covering ~2.5. Mbp was identified by measured genotype analysis, although none of the SNPs reached genome-wide significance. To validate the putative quantitative trait locus, we genotyped two of the SNPs within the region in unrelated, informative animals exposed to experimental or natural infections and from which we had worm counts and/or faecal egg counts; the validation studies showed that one of the SNPs (TXNIP) was associated with total worm burden (. P<. 0.001) and adult worm burden (. P<. 0.0001), whereas the other SNP (ARNT) was associated with adult worm burden (. P<. 0.025) in these populations. We were thus able to confirm the existence of the quantitative trait locus on chromosome 4. This is to our knowledge the first report of a quantitative trait locus associated with helminth burden in pigs.

Original language	English
Journal	International Journal for Parasitology
Volume	42
Issue number	4
Pages (from-to)	383-391
Number of pages	9
ISSN	0020-7519
DOIs	https://doi.org/10.1016/j.ijpara.2012.02.010
Publication status	Published - Apr 2012

Keywords

Former LIFE faculty

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10.1016/j.ijpara.2012.02.010

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title = "Detection of a quantitative trait locus associated with resistance to Ascaris suum infection in pigs",

abstract = "Helminths almost invariably have an over-dispersed distribution in the host population. Human and animal studies have provided evidence suggesting that a large part of this variation is due to host genetic factors. Recently, the heritability for roundworm (. Ascaris suum) infection levels in pigs was estimated to be 0.45. We used single nucleotide polymorphism markers to perform a whole-genome scan on 195 pigs experimentally infected with . A. suum. A putative quantitative trait locus for worm burden on chromosome 4 covering ~2.5. Mbp was identified by measured genotype analysis, although none of the SNPs reached genome-wide significance. To validate the putative quantitative trait locus, we genotyped two of the SNPs within the region in unrelated, informative animals exposed to experimental or natural infections and from which we had worm counts and/or faecal egg counts; the validation studies showed that one of the SNPs (TXNIP) was associated with total worm burden (. P<. 0.001) and adult worm burden (. P<. 0.0001), whereas the other SNP (ARNT) was associated with adult worm burden (. P<. 0.025) in these populations. We were thus able to confirm the existence of the quantitative trait locus on chromosome 4. This is to our knowledge the first report of a quantitative trait locus associated with helminth burden in pigs.",

keywords = "Former LIFE faculty, Ascaris suum, Quantitative trait locusSingle nucleotide polymorphismPigHeritability, Immunity, Host genetic",

author = "Per Skallerup and Peter Nejsum and J{\o}rgensen, {Claus B{\o}ttcher} and G{\"o}ring, {Harald H. H.} and Peter Karlskov-Mortensen and Archibald, {Alan L.} and Merete Fredholm and Thamsborg, {Stig Milan}",

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language = "English",

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T1 - Detection of a quantitative trait locus associated with resistance to Ascaris suum infection in pigs

AU - Skallerup, Per

AU - Nejsum, Peter

AU - Jørgensen, Claus Bøttcher

AU - Göring, Harald H. H.

AU - Karlskov-Mortensen, Peter

AU - Archibald, Alan L.

AU - Fredholm, Merete

AU - Thamsborg, Stig Milan

PY - 2012/4

Y1 - 2012/4

N2 - Helminths almost invariably have an over-dispersed distribution in the host population. Human and animal studies have provided evidence suggesting that a large part of this variation is due to host genetic factors. Recently, the heritability for roundworm (. Ascaris suum) infection levels in pigs was estimated to be 0.45. We used single nucleotide polymorphism markers to perform a whole-genome scan on 195 pigs experimentally infected with . A. suum. A putative quantitative trait locus for worm burden on chromosome 4 covering ~2.5. Mbp was identified by measured genotype analysis, although none of the SNPs reached genome-wide significance. To validate the putative quantitative trait locus, we genotyped two of the SNPs within the region in unrelated, informative animals exposed to experimental or natural infections and from which we had worm counts and/or faecal egg counts; the validation studies showed that one of the SNPs (TXNIP) was associated with total worm burden (. P<. 0.001) and adult worm burden (. P<. 0.0001), whereas the other SNP (ARNT) was associated with adult worm burden (. P<. 0.025) in these populations. We were thus able to confirm the existence of the quantitative trait locus on chromosome 4. This is to our knowledge the first report of a quantitative trait locus associated with helminth burden in pigs.

AB - Helminths almost invariably have an over-dispersed distribution in the host population. Human and animal studies have provided evidence suggesting that a large part of this variation is due to host genetic factors. Recently, the heritability for roundworm (. Ascaris suum) infection levels in pigs was estimated to be 0.45. We used single nucleotide polymorphism markers to perform a whole-genome scan on 195 pigs experimentally infected with . A. suum. A putative quantitative trait locus for worm burden on chromosome 4 covering ~2.5. Mbp was identified by measured genotype analysis, although none of the SNPs reached genome-wide significance. To validate the putative quantitative trait locus, we genotyped two of the SNPs within the region in unrelated, informative animals exposed to experimental or natural infections and from which we had worm counts and/or faecal egg counts; the validation studies showed that one of the SNPs (TXNIP) was associated with total worm burden (. P<. 0.001) and adult worm burden (. P<. 0.0001), whereas the other SNP (ARNT) was associated with adult worm burden (. P<. 0.025) in these populations. We were thus able to confirm the existence of the quantitative trait locus on chromosome 4. This is to our knowledge the first report of a quantitative trait locus associated with helminth burden in pigs.

KW - Former LIFE faculty

KW - Ascaris suum

KW - Quantitative trait locusSingle nucleotide polymorphismPigHeritability

KW - Immunity

KW - Host genetic

U2 - 10.1016/j.ijpara.2012.02.010

DO - 10.1016/j.ijpara.2012.02.010

M3 - Journal article

SN - 0020-7519

VL - 42

SP - 383

EP - 391

JO - International Journal for Parasitology

JF - International Journal for Parasitology

IS - 4

ER -

Detection of a quantitative trait locus associated with resistance to Ascaris suum infection in pigs

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