Design, synthesis and in vitro pharmacology of GluK1 and GluK3 antagonists. Studies towards the design of subtype-selective antagonists through 2-carboxyethyl-phenylalanines with substituents interacting with non-conserved residues in the GluK binding sites

Niklas Sköld; Birgitte Nielsen; Jakob Olsen; Liwei Han; Lars Olsen; Ulf Madsen; Jesper Langgaard Kristensen; Darryl S Pickering; Tommy N Johansen

doi:10.1016/j.bmc.2014.07.045

Design, synthesis and in vitro pharmacology of GluK1 and GluK3 antagonists. Studies towards the design of subtype-selective antagonists through 2-carboxyethyl-phenylalanines with substituents interacting with non-conserved residues in the GluK binding sites

Niklas Sköld, Birgitte Nielsen, Jakob Olsen, Liwei Han, Lars Olsen, Ulf Madsen, Jesper Langgaard Kristensen, Darryl S Pickering, Tommy N Johansen

4 Citations (Scopus)

Abstract

In order to identify compounds selective for the GluK1 and GluK3 subtypes of kainate receptors we have designed and synthesized a series of (S)-2-amino-3-((2-carboxyethyl)phenyl)propanoic acid analogs with hydrogen bond donating and accepting substituents on the aromatic ring. Based on crystal structures of GluK1 in complex with related ligands, the compounds were designed to explore possible interactions with non-conserved residues outside the glutamate ligand binding site and challenge the water binding network. Apart from obtaining GluK1 selective antagonists one analog with a phenyl-substituted urea (compound 31) showed some preference for GluK3 over GluK1-receptors. Docking studies indicate that this preference may be attributed to contacts between the NH of the urea substituent and non-conserved Ser741 and Ser761 residues.

Original language	English
Journal	Bioorganic & Medicinal Chemistry
Volume	22
Issue number	19
Pages (from-to)	5368-5377
Number of pages	10
ISSN	0968-0896
DOIs	https://doi.org/10.1016/j.bmc.2014.07.045
Publication status	Published - 1 Oct 2014

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Sköld, N., Nielsen, B., Olsen, J., Han, L., Olsen, L., Madsen, U., Kristensen, J. L., Pickering, D. S., & Johansen, T. N. (2014). Design, synthesis and in vitro pharmacology of GluK1 and GluK3 antagonists. Studies towards the design of subtype-selective antagonists through 2-carboxyethyl-phenylalanines with substituents interacting with non-conserved residues in the GluK binding sites. Bioorganic & Medicinal Chemistry, 22(19), 5368-5377. https://doi.org/10.1016/j.bmc.2014.07.045

Design, synthesis and in vitro pharmacology of GluK1 and GluK3 antagonists. Studies towards the design of subtype-selective antagonists through 2-carboxyethyl-phenylalanines with substituents interacting with non-conserved residues in the GluK binding sites. / Sköld, Niklas; Nielsen, Birgitte; Olsen, Jakob et al.

In: Bioorganic & Medicinal Chemistry, Vol. 22, No. 19, 01.10.2014, p. 5368-5377.

Research output: Contribution to journal › Journal article › Research › peer-review

Sköld, N, Nielsen, B, Olsen, J, Han, L, Olsen, L, Madsen, U , Kristensen, JL , Pickering, DS & Johansen, TN 2014, 'Design, synthesis and in vitro pharmacology of GluK1 and GluK3 antagonists. Studies towards the design of subtype-selective antagonists through 2-carboxyethyl-phenylalanines with substituents interacting with non-conserved residues in the GluK binding sites', Bioorganic & Medicinal Chemistry, vol. 22, no. 19, pp. 5368-5377. https://doi.org/10.1016/j.bmc.2014.07.045

Sköld N, Nielsen B, Olsen J, Han L, Olsen L, Madsen U et al. Design, synthesis and in vitro pharmacology of GluK1 and GluK3 antagonists. Studies towards the design of subtype-selective antagonists through 2-carboxyethyl-phenylalanines with substituents interacting with non-conserved residues in the GluK binding sites. Bioorganic & Medicinal Chemistry. 2014 Oct 1;22(19):5368-5377. doi: 10.1016/j.bmc.2014.07.045

Sköld, Niklas ; Nielsen, Birgitte ; Olsen, Jakob et al. / Design, synthesis and in vitro pharmacology of GluK1 and GluK3 antagonists. Studies towards the design of subtype-selective antagonists through 2-carboxyethyl-phenylalanines with substituents interacting with non-conserved residues in the GluK binding sites. In: Bioorganic & Medicinal Chemistry. 2014 ; Vol. 22, No. 19. pp. 5368-5377.

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title = "Design, synthesis and in vitro pharmacology of GluK1 and GluK3 antagonists. Studies towards the design of subtype-selective antagonists through 2-carboxyethyl-phenylalanines with substituents interacting with non-conserved residues in the GluK binding sites",

abstract = "In order to identify compounds selective for the GluK1 and GluK3 subtypes of kainate receptors we have designed and synthesized a series of (S)-2-amino-3-((2-carboxyethyl)phenyl)propanoic acid analogs with hydrogen bond donating and accepting substituents on the aromatic ring. Based on crystal structures of GluK1 in complex with related ligands, the compounds were designed to explore possible interactions with non-conserved residues outside the glutamate ligand binding site and challenge the water binding network. Apart from obtaining GluK1 selective antagonists one analog with a phenyl-substituted urea (compound 31) showed some preference for GluK3 over GluK1-receptors. Docking studies indicate that this preference may be attributed to contacts between the NH of the urea substituent and non-conserved Ser741 and Ser761 residues.",

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AU - Nielsen, Birgitte

AU - Olsen, Jakob

AU - Han, Liwei

AU - Olsen, Lars

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AB - In order to identify compounds selective for the GluK1 and GluK3 subtypes of kainate receptors we have designed and synthesized a series of (S)-2-amino-3-((2-carboxyethyl)phenyl)propanoic acid analogs with hydrogen bond donating and accepting substituents on the aromatic ring. Based on crystal structures of GluK1 in complex with related ligands, the compounds were designed to explore possible interactions with non-conserved residues outside the glutamate ligand binding site and challenge the water binding network. Apart from obtaining GluK1 selective antagonists one analog with a phenyl-substituted urea (compound 31) showed some preference for GluK3 over GluK1-receptors. Docking studies indicate that this preference may be attributed to contacts between the NH of the urea substituent and non-conserved Ser741 and Ser761 residues.

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