Design aspects of bright red emissive silver nanoclusters/DNA probes for microRNA detection

Pratik Shah; Andreas Rørvig-Lund; Samir Ben Chaabane; Peter Waaben Thulstrup; Henrik Grum Kjaergaard; Eduard Fron; Johan Hofkens; Seong Wook Yang; Tom André Jos Vosch

doi:10.1021/nn302633q

Design aspects of bright red emissive silver nanoclusters/DNA probes for microRNA detection

Pratik Shah, Andreas Rørvig-Lund, Samir Ben Chaabane, Peter Waaben Thulstrup, Henrik Grum Kjaergaard, Eduard Fron, Johan Hofkens, Seong Wook Yang, Tom André Jos Vosch

Department of Chemistry

149 Citations (Scopus)

Abstract

The influence of the nucleic acid secondary structure on the fast (1 h) formation of bright red emissive silver nanoclusters (AgNCs) in a DNA sequence (DNA-12nt-RED-160), designed for the detection of a microRNA sequence (RNA-miR160), was investigated. The findings show that especially the propensity for mismatch self-dimer formation of the DNA probes can be a good indicator for the creation and stabilization of red emissive AgNCs. Also, the role of the thermal stability of the secondary DNA structures (mismatch self-dimer and hairpin monomers) and the observed AgNC red emission intensity were investigated. These findings can form the basis for a rationale to design new red emissive AgNC-based probes. As an example, a bright red emissive AgNC-based DNA probe was designed for RNA-miR172 detection. The latter opens the possibility to create a variety of AgNC-based DNA probes for the specific detection of plant and animal miRNAs.

Original language	English
Journal	A C S Nano
Volume	6
Issue number	10
Pages (from-to)	8803–8814
Number of pages	12
ISSN	1936-0851
DOIs	https://doi.org/10.1021/nn302633q
Publication status	Published - 23 Oct 2012

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http://pubs.acs.org/doi/abs/10.1021/nn302633q

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title = "Design aspects of bright red emissive silver nanoclusters/DNA probes for microRNA detection",

abstract = "The influence of the nucleic acid secondary structure on the fast (1 h) formation of bright red emissive silver nanoclusters (AgNCs) in a DNA sequence (DNA-12nt-RED-160), designed for the detection of a microRNA sequence (RNA-miR160), was investigated. The findings show that especially the propensity for mismatch self-dimer formation of the DNA probes can be a good indicator for the creation and stabilization of red emissive AgNCs. Also, the role of the thermal stability of the secondary DNA structures (mismatch self-dimer and hairpin monomers) and the observed AgNC red emission intensity were investigated. These findings can form the basis for a rationale to design new red emissive AgNC-based probes. As an example, a bright red emissive AgNC-based DNA probe was designed for RNA-miR172 detection. The latter opens the possibility to create a variety of AgNC-based DNA probes for the specific detection of plant and animal miRNAs.",

author = "Pratik Shah and Andreas R{\o}rvig-Lund and Chaabane, {Samir Ben} and Thulstrup, {Peter Waaben} and Kjaergaard, {Henrik Grum} and Eduard Fron and Johan Hofkens and Yang, {Seong Wook} and Vosch, {Tom Andr{\'e} Jos}",

year = "2012",

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doi = "10.1021/nn302633q",

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T1 - Design aspects of bright red emissive silver nanoclusters/DNA probes for microRNA detection

AU - Shah, Pratik

AU - Rørvig-Lund, Andreas

AU - Chaabane, Samir Ben

AU - Thulstrup, Peter Waaben

AU - Kjaergaard, Henrik Grum

AU - Fron, Eduard

AU - Hofkens, Johan

AU - Yang, Seong Wook

AU - Vosch, Tom André Jos

PY - 2012/10/23

Y1 - 2012/10/23

N2 - The influence of the nucleic acid secondary structure on the fast (1 h) formation of bright red emissive silver nanoclusters (AgNCs) in a DNA sequence (DNA-12nt-RED-160), designed for the detection of a microRNA sequence (RNA-miR160), was investigated. The findings show that especially the propensity for mismatch self-dimer formation of the DNA probes can be a good indicator for the creation and stabilization of red emissive AgNCs. Also, the role of the thermal stability of the secondary DNA structures (mismatch self-dimer and hairpin monomers) and the observed AgNC red emission intensity were investigated. These findings can form the basis for a rationale to design new red emissive AgNC-based probes. As an example, a bright red emissive AgNC-based DNA probe was designed for RNA-miR172 detection. The latter opens the possibility to create a variety of AgNC-based DNA probes for the specific detection of plant and animal miRNAs.

AB - The influence of the nucleic acid secondary structure on the fast (1 h) formation of bright red emissive silver nanoclusters (AgNCs) in a DNA sequence (DNA-12nt-RED-160), designed for the detection of a microRNA sequence (RNA-miR160), was investigated. The findings show that especially the propensity for mismatch self-dimer formation of the DNA probes can be a good indicator for the creation and stabilization of red emissive AgNCs. Also, the role of the thermal stability of the secondary DNA structures (mismatch self-dimer and hairpin monomers) and the observed AgNC red emission intensity were investigated. These findings can form the basis for a rationale to design new red emissive AgNC-based probes. As an example, a bright red emissive AgNC-based DNA probe was designed for RNA-miR172 detection. The latter opens the possibility to create a variety of AgNC-based DNA probes for the specific detection of plant and animal miRNAs.

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DO - 10.1021/nn302633q

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JO - A C S Nano

JF - A C S Nano

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ER -

Design aspects of bright red emissive silver nanoclusters/DNA probes for microRNA detection

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