Desaturation of excess intramyocellular triacylglycerol in obesity: implications for glycemic control

S B Haugaard; S Madsbad; Huiling Mu; A Vaag

doi:10.1038/ijo.2009.264

Desaturation of excess intramyocellular triacylglycerol in obesity: implications for glycemic control

S B Haugaard, S Madsbad, Huiling Mu, A Vaag

5 Citations (Scopus)

Abstract

Objective:Excess intramyocellular triacylglycerol (IMTG), found especially in obese women, is slowly metabolized and, therefore, prone to longer exposure to intracellular desaturases. Accordingly, it was hypothesized that IMTG content correlates inversely with IMTG fatty acid (FA) saturation in sedentary subjects. In addition, it was validated if IMTG palmitic acid is associated with insulin resistance as suggested earlier.Design:Cross-sectional human study.Subjects:In skeletal muscle biopsies, which were obtained from sedentary subjects (34 women, age 48±2 years (27 obese including 7 type 2 diabetes (T2DM), body mass index (BMI)=35.5±0.8 kg m^-2) and 25 men, age 49±2 years (20 obese including 6 T2DM, BMI35.8±0.8 kg m ^-2)), IMTG FA composition was determined by gas-liquid chromatography after separation from phospholipids by thin-layer chromatography.Results: Independently of gender saturated FA correlated inversely with IMTG (P<0.001) and monounsaturated FA (P<0.001) including total unsaturation of FA (P<0.002) correlated positively with IMTG. Obese women exhibited lower total saturated FA (P<0.001) and palmitic acid (P<0.001) than obese men independent of IMTG, the latter of which, however, was increased twofold in obese women compared to obese men (P<0.001). Polyunsaturated and long-chain polyunsaturated FA did not correlate with IMTG. Palmitic acid correlated positively with insulin resistance (homeostasis insulin resistance index, P<0.05), fasting glucose (P<0.01) and glycosylated hemoglobin (P<0.002) both in univariate analysis and after correction for gender and IMTG.Conclusion:IMTG content correlates inversely with IMTG saturated FA, potentially reflecting a low turnover of excess IMTG prone to in situ desaturation probably by the ubiquitous stearoyl-CoA desaturase-1. IMTG FA composition is gender specific and implicates on insulin sensitivity and glycemic control.

Original language	English
Journal	International Journal of Obesity
Volume	34
Issue number	3
Pages (from-to)	500-510
Number of pages	10
ISSN	0307-0565
DOIs	https://doi.org/10.1038/ijo.2009.264
Publication status	Published - Mar 2010

Keywords

Former Faculty of Pharmaceutical Sciences

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10.1038/ijo.2009.264

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@article{74a27bd043c811df928f000ea68e967b,

title = "Desaturation of excess intramyocellular triacylglycerol in obesity: implications for glycemic control",

abstract = "Objective:Excess intramyocellular triacylglycerol (IMTG), found especially in obese women, is slowly metabolized and, therefore, prone to longer exposure to intracellular desaturases. Accordingly, it was hypothesized that IMTG content correlates inversely with IMTG fatty acid (FA) saturation in sedentary subjects. In addition, it was validated if IMTG palmitic acid is associated with insulin resistance as suggested earlier.Design:Cross-sectional human study.Subjects:In skeletal muscle biopsies, which were obtained from sedentary subjects (34 women, age 48±2 years (27 obese including 7 type 2 diabetes (T2DM), body mass index (BMI)=35.5±0.8 kg m-2) and 25 men, age 49±2 years (20 obese including 6 T2DM, BMI35.8±0.8 kg m -2)), IMTG FA composition was determined by gas-liquid chromatography after separation from phospholipids by thin-layer chromatography.Results: Independently of gender saturated FA correlated inversely with IMTG (P<0.001) and monounsaturated FA (P<0.001) including total unsaturation of FA (P<0.002) correlated positively with IMTG. Obese women exhibited lower total saturated FA (P<0.001) and palmitic acid (P<0.001) than obese men independent of IMTG, the latter of which, however, was increased twofold in obese women compared to obese men (P<0.001). Polyunsaturated and long-chain polyunsaturated FA did not correlate with IMTG. Palmitic acid correlated positively with insulin resistance (homeostasis insulin resistance index, P<0.05), fasting glucose (P<0.01) and glycosylated hemoglobin (P<0.002) both in univariate analysis and after correction for gender and IMTG.Conclusion:IMTG content correlates inversely with IMTG saturated FA, potentially reflecting a low turnover of excess IMTG prone to in situ desaturation probably by the ubiquitous stearoyl-CoA desaturase-1. IMTG FA composition is gender specific and implicates on insulin sensitivity and glycemic control.",

keywords = "Former Faculty of Pharmaceutical Sciences",

author = "Haugaard, {S B} and S Madsbad and Huiling Mu and A Vaag",

year = "2010",

month = mar,

doi = "10.1038/ijo.2009.264",

language = "English",

volume = "34",

pages = "500--510",

journal = "International Journal of Obesity",

issn = "0307-0565",

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TY - JOUR

T1 - Desaturation of excess intramyocellular triacylglycerol in obesity

T2 - implications for glycemic control

AU - Haugaard, S B

AU - Madsbad, S

AU - Mu, Huiling

AU - Vaag, A

PY - 2010/3

Y1 - 2010/3

N2 - Objective:Excess intramyocellular triacylglycerol (IMTG), found especially in obese women, is slowly metabolized and, therefore, prone to longer exposure to intracellular desaturases. Accordingly, it was hypothesized that IMTG content correlates inversely with IMTG fatty acid (FA) saturation in sedentary subjects. In addition, it was validated if IMTG palmitic acid is associated with insulin resistance as suggested earlier.Design:Cross-sectional human study.Subjects:In skeletal muscle biopsies, which were obtained from sedentary subjects (34 women, age 48±2 years (27 obese including 7 type 2 diabetes (T2DM), body mass index (BMI)=35.5±0.8 kg m-2) and 25 men, age 49±2 years (20 obese including 6 T2DM, BMI35.8±0.8 kg m -2)), IMTG FA composition was determined by gas-liquid chromatography after separation from phospholipids by thin-layer chromatography.Results: Independently of gender saturated FA correlated inversely with IMTG (P<0.001) and monounsaturated FA (P<0.001) including total unsaturation of FA (P<0.002) correlated positively with IMTG. Obese women exhibited lower total saturated FA (P<0.001) and palmitic acid (P<0.001) than obese men independent of IMTG, the latter of which, however, was increased twofold in obese women compared to obese men (P<0.001). Polyunsaturated and long-chain polyunsaturated FA did not correlate with IMTG. Palmitic acid correlated positively with insulin resistance (homeostasis insulin resistance index, P<0.05), fasting glucose (P<0.01) and glycosylated hemoglobin (P<0.002) both in univariate analysis and after correction for gender and IMTG.Conclusion:IMTG content correlates inversely with IMTG saturated FA, potentially reflecting a low turnover of excess IMTG prone to in situ desaturation probably by the ubiquitous stearoyl-CoA desaturase-1. IMTG FA composition is gender specific and implicates on insulin sensitivity and glycemic control.

AB - Objective:Excess intramyocellular triacylglycerol (IMTG), found especially in obese women, is slowly metabolized and, therefore, prone to longer exposure to intracellular desaturases. Accordingly, it was hypothesized that IMTG content correlates inversely with IMTG fatty acid (FA) saturation in sedentary subjects. In addition, it was validated if IMTG palmitic acid is associated with insulin resistance as suggested earlier.Design:Cross-sectional human study.Subjects:In skeletal muscle biopsies, which were obtained from sedentary subjects (34 women, age 48±2 years (27 obese including 7 type 2 diabetes (T2DM), body mass index (BMI)=35.5±0.8 kg m-2) and 25 men, age 49±2 years (20 obese including 6 T2DM, BMI35.8±0.8 kg m -2)), IMTG FA composition was determined by gas-liquid chromatography after separation from phospholipids by thin-layer chromatography.Results: Independently of gender saturated FA correlated inversely with IMTG (P<0.001) and monounsaturated FA (P<0.001) including total unsaturation of FA (P<0.002) correlated positively with IMTG. Obese women exhibited lower total saturated FA (P<0.001) and palmitic acid (P<0.001) than obese men independent of IMTG, the latter of which, however, was increased twofold in obese women compared to obese men (P<0.001). Polyunsaturated and long-chain polyunsaturated FA did not correlate with IMTG. Palmitic acid correlated positively with insulin resistance (homeostasis insulin resistance index, P<0.05), fasting glucose (P<0.01) and glycosylated hemoglobin (P<0.002) both in univariate analysis and after correction for gender and IMTG.Conclusion:IMTG content correlates inversely with IMTG saturated FA, potentially reflecting a low turnover of excess IMTG prone to in situ desaturation probably by the ubiquitous stearoyl-CoA desaturase-1. IMTG FA composition is gender specific and implicates on insulin sensitivity and glycemic control.

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1038/ijo.2009.264

DO - 10.1038/ijo.2009.264

M3 - Journal article

C2 - 20029372

SN - 0307-0565

VL - 34

SP - 500

EP - 510

JO - International Journal of Obesity

JF - International Journal of Obesity

IS - 3