Depletion of the AP-1 repressor JDP2 induces cell death similar to apoptosis

Mads Lerdrup; Christian Henrik Holmberg; Nikolaj Dietrich; E. Shaulian; T. Herdegen; Marja Jaattela; T. Kallunki

doi:10.1016/j.bbamcr.2005.06.008

Depletion of the AP-1 repressor JDP2 induces cell death similar to apoptosis

Mads Lerdrup, Christian Henrik Holmberg, Nikolaj Dietrich, E. Shaulian, T. Herdegen, Marja Jaattela, T. Kallunki

20 Citations (Scopus)

Abstract

JDP2 is a ubiquitously expressed nuclear protein that efficiently represses the activity of the transcription factor AP-1. Thus far, all studies of JDP2 function have relied on the ectopic expression of the protein. In this study, we use a different approach: depletion of JDP2 from cells. Specific depletion of JDP2 resulted in p53-independent cell death that resembles apoptosis and was evident at 72 h. The death mechanism was caspase dependent as the cells could be rescued by treatment with caspase inhibitor zVAD. Our studies suggest that JDP2 functions as a general survival protein, not only following UV-irradiation, as reported earlier, but also under normal culture conditions. Thus, our data support that JDP2 is a cellular survival protein whose presence is necessary for normal cellular function.

Original language	English
Journal	Biochimica et Biophysica Acta - Molecular Cell Research
Volume	1745
Issue number	1
Pages (from-to)	29-37
ISSN	0167-4889
DOIs	https://doi.org/10.1016/j.bbamcr.2005.06.008
Publication status	Published - 2005

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10.1016/j.bbamcr.2005.06.008

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title = "Depletion of the AP-1 repressor JDP2 induces cell death similar to apoptosis",

abstract = "JDP2 is a ubiquitously expressed nuclear protein that efficiently represses the activity of the transcription factor AP-1. Thus far, all studies of JDP2 function have relied on the ectopic expression of the protein. In this study, we use a different approach: depletion of JDP2 from cells. Specific depletion of JDP2 resulted in p53-independent cell death that resembles apoptosis and was evident at 72 h. The death mechanism was caspase dependent as the cells could be rescued by treatment with caspase inhibitor zVAD. Our studies suggest that JDP2 functions as a general survival protein, not only following UV-irradiation, as reported earlier, but also under normal culture conditions. Thus, our data support that JDP2 is a cellular survival protein whose presence is necessary for normal cellular function.",

author = "Mads Lerdrup and Holmberg, {Christian Henrik} and Nikolaj Dietrich and E. Shaulian and T. Herdegen and Marja Jaattela and T. Kallunki",

note = "Keywords: JDP2; AP-1; Programmed cell death; Caspase; Antisense",

year = "2005",

doi = "10.1016/j.bbamcr.2005.06.008",

language = "English",

volume = "1745",

pages = "29--37",

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TY - JOUR

T1 - Depletion of the AP-1 repressor JDP2 induces cell death similar to apoptosis

AU - Lerdrup, Mads

AU - Holmberg, Christian Henrik

AU - Dietrich, Nikolaj

AU - Shaulian, E.

AU - Herdegen, T.

AU - Jaattela, Marja

AU - Kallunki, T.

N1 - Keywords: JDP2; AP-1; Programmed cell death; Caspase; Antisense

PY - 2005

Y1 - 2005

N2 - JDP2 is a ubiquitously expressed nuclear protein that efficiently represses the activity of the transcription factor AP-1. Thus far, all studies of JDP2 function have relied on the ectopic expression of the protein. In this study, we use a different approach: depletion of JDP2 from cells. Specific depletion of JDP2 resulted in p53-independent cell death that resembles apoptosis and was evident at 72 h. The death mechanism was caspase dependent as the cells could be rescued by treatment with caspase inhibitor zVAD. Our studies suggest that JDP2 functions as a general survival protein, not only following UV-irradiation, as reported earlier, but also under normal culture conditions. Thus, our data support that JDP2 is a cellular survival protein whose presence is necessary for normal cellular function.

AB - JDP2 is a ubiquitously expressed nuclear protein that efficiently represses the activity of the transcription factor AP-1. Thus far, all studies of JDP2 function have relied on the ectopic expression of the protein. In this study, we use a different approach: depletion of JDP2 from cells. Specific depletion of JDP2 resulted in p53-independent cell death that resembles apoptosis and was evident at 72 h. The death mechanism was caspase dependent as the cells could be rescued by treatment with caspase inhibitor zVAD. Our studies suggest that JDP2 functions as a general survival protein, not only following UV-irradiation, as reported earlier, but also under normal culture conditions. Thus, our data support that JDP2 is a cellular survival protein whose presence is necessary for normal cellular function.

U2 - 10.1016/j.bbamcr.2005.06.008

DO - 10.1016/j.bbamcr.2005.06.008

M3 - Journal article

C2 - 16026868

SN - 0167-4889

VL - 1745

SP - 29

EP - 37

JO - B B A - Molecular Cell Research

JF - B B A - Molecular Cell Research

IS - 1

ER -

Depletion of the AP-1 repressor JDP2 induces cell death similar to apoptosis

Abstract

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