Dendrite-derived supernumerary axons on adult axotomized motor neurons possess proteins that are essential for the initiation and propagation of action potentials and synaptic vesicle release

Claire Francesca Meehan; Victoria E MacDermid; Steven J Montague; Monica Neuber-Hess; P Ken Rose

doi:10.1523/JNEUROSCI.5377-10.2011

Dendrite-derived supernumerary axons on adult axotomized motor neurons possess proteins that are essential for the initiation and propagation of action potentials and synaptic vesicle release

Claire Francesca Meehan, Victoria E MacDermid, Steven J Montague, Monica Neuber-Hess, P Ken Rose

Motor Control

7 Citations (Scopus)

Abstract

Axotomy can trigger profound alterations in the neuronal polarity of adult neurons in vivo. This can manifest itself in the development of new axon-like processes emanating from the tips of distal dendrites. Previously, these processes have been defined as axonal based on their axonal morphology. This study extends this definition to determine whether, more importantly, these processes possess the prerequisite molecular machinery to function as axons. Using a combination of intracellular labeling and immunohistochemistry, we demonstrate that the distribution of voltage-gated sodium channels on these processes matches the arrangement of these channels that is necessary for the initiation and conduction of action potentials. At terminal bouton-like structures they possess key proteins necessary for the release of synaptic vesicles (SV2 and synaptophysin). Thus, axon-like processes emanating from the tips of distal dendrites represent a rearrangement of neuronal polarity whereby axotomized neurons can develop additional functional axons in vivo.

Original language	English
Journal	Journal of Neuroscience
Volume	31
Issue number	18
Pages (from-to)	6732-40
Number of pages	9
ISSN	0270-6474
DOIs	https://doi.org/10.1523/JNEUROSCI.5377-10.2011
Publication status	Published - 4 May 2011

Keywords

Action Potentials
Animals
Axons
Axotomy
Cats
Dendrites
Immunohistochemistry
Motor Neurons
Sodium Channels
Synapses
Synaptic Transmission
Synaptic Vesicles
Synaptophysin

Access to Document

10.1523/JNEUROSCI.5377-10.2011

Cite this

Meehan, C. F., MacDermid, V. E., Montague, S. J., Neuber-Hess, M., & Rose, P. K. (2011). Dendrite-derived supernumerary axons on adult axotomized motor neurons possess proteins that are essential for the initiation and propagation of action potentials and synaptic vesicle release. Journal of Neuroscience, 31(18), 6732-40. https://doi.org/10.1523/JNEUROSCI.5377-10.2011

Dendrite-derived supernumerary axons on adult axotomized motor neurons possess proteins that are essential for the initiation and propagation of action potentials and synaptic vesicle release. / Meehan, Claire Francesca; MacDermid, Victoria E; Montague, Steven J et al.
In: Journal of Neuroscience, Vol. 31, No. 18, 04.05.2011, p. 6732-40.

Research output: Contribution to journal › Journal article › Research › peer-review

@article{6ecf2141dd5b48f786617dc7dd633302,

title = "Dendrite-derived supernumerary axons on adult axotomized motor neurons possess proteins that are essential for the initiation and propagation of action potentials and synaptic vesicle release",

abstract = "Axotomy can trigger profound alterations in the neuronal polarity of adult neurons in vivo. This can manifest itself in the development of new axon-like processes emanating from the tips of distal dendrites. Previously, these processes have been defined as axonal based on their axonal morphology. This study extends this definition to determine whether, more importantly, these processes possess the prerequisite molecular machinery to function as axons. Using a combination of intracellular labeling and immunohistochemistry, we demonstrate that the distribution of voltage-gated sodium channels on these processes matches the arrangement of these channels that is necessary for the initiation and conduction of action potentials. At terminal bouton-like structures they possess key proteins necessary for the release of synaptic vesicles (SV2 and synaptophysin). Thus, axon-like processes emanating from the tips of distal dendrites represent a rearrangement of neuronal polarity whereby axotomized neurons can develop additional functional axons in vivo.",

keywords = "Action Potentials, Animals, Axons, Axotomy, Cats, Dendrites, Immunohistochemistry, Motor Neurons, Sodium Channels, Synapses, Synaptic Transmission, Synaptic Vesicles, Synaptophysin",

author = "Meehan, {Claire Francesca} and MacDermid, {Victoria E} and Montague, {Steven J} and Monica Neuber-Hess and Rose, {P Ken}",

year = "2011",

month = may,

day = "4",

doi = "10.1523/JNEUROSCI.5377-10.2011",

language = "English",

volume = "31",

pages = "6732--40",

journal = "Journal of Neuroscience",

issn = "0270-6474",

publisher = "Society for Neuroscience",

number = "18",

}

TY - JOUR

T1 - Dendrite-derived supernumerary axons on adult axotomized motor neurons possess proteins that are essential for the initiation and propagation of action potentials and synaptic vesicle release

AU - Meehan, Claire Francesca

AU - MacDermid, Victoria E

AU - Montague, Steven J

AU - Neuber-Hess, Monica

AU - Rose, P Ken

PY - 2011/5/4

Y1 - 2011/5/4

N2 - Axotomy can trigger profound alterations in the neuronal polarity of adult neurons in vivo. This can manifest itself in the development of new axon-like processes emanating from the tips of distal dendrites. Previously, these processes have been defined as axonal based on their axonal morphology. This study extends this definition to determine whether, more importantly, these processes possess the prerequisite molecular machinery to function as axons. Using a combination of intracellular labeling and immunohistochemistry, we demonstrate that the distribution of voltage-gated sodium channels on these processes matches the arrangement of these channels that is necessary for the initiation and conduction of action potentials. At terminal bouton-like structures they possess key proteins necessary for the release of synaptic vesicles (SV2 and synaptophysin). Thus, axon-like processes emanating from the tips of distal dendrites represent a rearrangement of neuronal polarity whereby axotomized neurons can develop additional functional axons in vivo.

AB - Axotomy can trigger profound alterations in the neuronal polarity of adult neurons in vivo. This can manifest itself in the development of new axon-like processes emanating from the tips of distal dendrites. Previously, these processes have been defined as axonal based on their axonal morphology. This study extends this definition to determine whether, more importantly, these processes possess the prerequisite molecular machinery to function as axons. Using a combination of intracellular labeling and immunohistochemistry, we demonstrate that the distribution of voltage-gated sodium channels on these processes matches the arrangement of these channels that is necessary for the initiation and conduction of action potentials. At terminal bouton-like structures they possess key proteins necessary for the release of synaptic vesicles (SV2 and synaptophysin). Thus, axon-like processes emanating from the tips of distal dendrites represent a rearrangement of neuronal polarity whereby axotomized neurons can develop additional functional axons in vivo.

KW - Action Potentials

KW - Animals

KW - Axons

KW - Axotomy

KW - Cats

KW - Dendrites

KW - Immunohistochemistry

KW - Motor Neurons

KW - Sodium Channels

KW - Synapses

KW - Synaptic Transmission

KW - Synaptic Vesicles

KW - Synaptophysin

U2 - 10.1523/JNEUROSCI.5377-10.2011

DO - 10.1523/JNEUROSCI.5377-10.2011

M3 - Journal article

C2 - 21543602

SN - 0270-6474

VL - 31

SP - 6732

EP - 6740

JO - Journal of Neuroscience

JF - Journal of Neuroscience

IS - 18

ER -

Dendrite-derived supernumerary axons on adult axotomized motor neurons possess proteins that are essential for the initiation and propagation of action potentials and synaptic vesicle release

Abstract

Keywords

Access to Document

Fingerprint

Cite this