Delta-interacting Protein A, a New Inhibitory Partner of CCAAT/Enhancer-binding Protein ß, Implicated in Adipocyte Differentiation

TY - JOUR

T1 - Delta-interacting Protein A, a New Inhibitory Partner of CCAAT/Enhancer-binding Protein ß, Implicated in Adipocyte Differentiation

AU - Bezy, Olivier

AU - Elabd, Christian

AU - Cochet, Olivia

AU - Petersen, Rasmus Koefoed

AU - Arkowitz, R.

AU - Kristiansen, Karsten

AU - fl., m.

PY - 2005

Y1 - 2005

N2 - CCAAT/enhancer-binding protein ß (C/EBPß) is expressed early during the adipocyte differentiation program and plays an important role in this process. In an attempt to identify novel proteins that interact with C/EBPß, we performed a yeast two-hybrid screen with a preadipocyte cDNA library and identified a new co-regulator, delta-interacting protein A (DIPA). DIPA mRNA is expressed during adipocyte differentiation of clonal cell lines. DIPA interacts with C/EBPß and -d proteins in intact cells and inhibits their transcriptional activity but not that of C/EBPa. Stable overexpression of DIPA in preadipocytes partially inhibits adipocyte differentiation, whereas its gene silencing enhances this process. DIPA and C/EBPß co-localize in the nucleus, and overexpression of DIPA in preadipocytes results in a partial inhibition of the mitotic clonal expansion which is critical for differentiation. Thus, DIPA is a novel partner of C/EBPß that down-regulates early events of adipogenesis.

AB - CCAAT/enhancer-binding protein ß (C/EBPß) is expressed early during the adipocyte differentiation program and plays an important role in this process. In an attempt to identify novel proteins that interact with C/EBPß, we performed a yeast two-hybrid screen with a preadipocyte cDNA library and identified a new co-regulator, delta-interacting protein A (DIPA). DIPA mRNA is expressed during adipocyte differentiation of clonal cell lines. DIPA interacts with C/EBPß and -d proteins in intact cells and inhibits their transcriptional activity but not that of C/EBPa. Stable overexpression of DIPA in preadipocytes partially inhibits adipocyte differentiation, whereas its gene silencing enhances this process. DIPA and C/EBPß co-localize in the nucleus, and overexpression of DIPA in preadipocytes results in a partial inhibition of the mitotic clonal expansion which is critical for differentiation. Thus, DIPA is a novel partner of C/EBPß that down-regulates early events of adipogenesis.

U2 - 10.1074/jbc.M411741200

DO - 10.1074/jbc.M411741200

M3 - Journal article

C2 - 15644333

SN - 0021-9258

VL - 280

SP - 11432

EP - 11438

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 12

ER -