Delivery of differentiation factors by mesoporous silica particles assists advanced differentiation of transplanted murine embryonic stem cells

TY - JOUR

T1 - Delivery of differentiation factors by mesoporous silica particles assists advanced differentiation of transplanted murine embryonic stem cells

AU - Garcia-Bennett, Alfonso E

AU - Kozhevnikova, Mariya

AU - König, Niclas

AU - Zhou, Chunfang

AU - Leao, Richardson

AU - Knöpfel, Thomas

AU - Pankratova, Stanislava

AU - Trolle, Carl

AU - Berezin, Vladimir

AU - Bock, Elisabeth

AU - Aldskogius, Håkan

AU - Kozlova, Elena N

PY - 2013/11

Y1 - 2013/11

N2 - Stem cell transplantation holds great hope for the replacement of damaged cells in the nervous system. However, poor long-term survival after transplantation and insufficiently robust differentiation of stem cells into specialized cell types in vivo remain major obstacles for clinical application. Here, we report the development of a novel technological approach for the local delivery of exogenous trophic factor mimetics to transplanted cells using specifically designed silica nanoporous particles. We demonstrated that delivering Cintrofin and Gliafin, established peptide mimetics of the ciliary neurotrophic factor and glial cell line-derived neurotrophic factor, respectively, with these particles enabled not only robust functional differentiation of motor neurons from transplanted embryonic stem cells but also their long-term survival in vivo. We propose that the delivery of growth factors by mesoporous nanoparticles is a potentially versatile and widely applicable strategy for efficient differentiation and functional integration of stem cell derivatives upon transplantation.

AB - Stem cell transplantation holds great hope for the replacement of damaged cells in the nervous system. However, poor long-term survival after transplantation and insufficiently robust differentiation of stem cells into specialized cell types in vivo remain major obstacles for clinical application. Here, we report the development of a novel technological approach for the local delivery of exogenous trophic factor mimetics to transplanted cells using specifically designed silica nanoporous particles. We demonstrated that delivering Cintrofin and Gliafin, established peptide mimetics of the ciliary neurotrophic factor and glial cell line-derived neurotrophic factor, respectively, with these particles enabled not only robust functional differentiation of motor neurons from transplanted embryonic stem cells but also their long-term survival in vivo. We propose that the delivery of growth factors by mesoporous nanoparticles is a potentially versatile and widely applicable strategy for efficient differentiation and functional integration of stem cell derivatives upon transplantation.

KW - Amino Acid Sequence

KW - Animals

KW - Cell Differentiation

KW - Cell Line

KW - Cell Survival

KW - Ciliary Neurotrophic Factor

KW - Embryonic Stem Cells

KW - Glial Cell Line-Derived Neurotrophic Factor

KW - Mice

KW - Molecular Sequence Data

KW - Motor Neurons

KW - Nanoparticles

KW - Peptides

KW - Silicon Dioxide

KW - Stem Cell Transplantation

U2 - 10.5966/sctm.2013-0072

DO - 10.5966/sctm.2013-0072

M3 - Journal article

C2 - 24089415

SN - 2157-6564

VL - 2

SP - 906

EP - 915

JO - Stem cells translational medicine

JF - Stem cells translational medicine

IS - 11

ER -