Decreased material-activation of the complement system using low-energy plasma polymerized poly(vinyl pyrrolidone) coatings

TY - JOUR

T1 - Decreased material-activation of the complement system using low-energy plasma polymerized poly(vinyl pyrrolidone) coatings

AU - Andersen, Thomas E

AU - Palarasah, Yaseelan

AU - Skjødt, Mikkel-Ole

AU - Ogaki, Ryosuke

AU - Benter, Maike

AU - Alei, Mojagan

AU - Kolmos, Hans J

AU - Koch, Claus

AU - Kingshott, Peter

N1 - Copyright © 2011 Elsevier Ltd. All rights reserved.

PY - 2011/7

Y1 - 2011/7

N2 - In the current study we investigate the activation of blood complement on medical device silicone rubber and present a plasma polymerized vinyl pyrrolidone (ppVP) coating which strongly decreases surface-activation of the blood complement system. We show that uncoated silicone and polystyrene are both potent activators of the complement system, measured both as activated, deposited C3b and quantifying fluid-phase release of the cleavage fragment C3c. The ppVP coated silicone exhibits approximately 90% reduced complement activation compared to untreated silicone. Quartz crystal microbalance with dissipation (QCM-D) measurements show relatively strong adsorption of blood proteins including native C3 to the ppVP surface, indicating that reduction of complement activation on ppVP is neither a result of low protein adsorption nor lower direct C3-binding, and is therefore possibly a consequence of differences in the adsorbed protein layer composition. The alternative and classical complement pathways are barely detectable on ppVP while the lectin pathway through MBL/ficolin-2 deposition remains active on ppVP suggesting this pathway is responsible for the remaining subtle activation on the ppVP coated surface. The ppVP surface is furthermore characterized physically and chemically using scanning electron microscopy (SEM), x-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR), which indicates preservation of chemical functionality by the applied plasma process. Overall, the ppVP coating shows a potential for increasing complement-compatibility of blood-contacting devices.

AB - In the current study we investigate the activation of blood complement on medical device silicone rubber and present a plasma polymerized vinyl pyrrolidone (ppVP) coating which strongly decreases surface-activation of the blood complement system. We show that uncoated silicone and polystyrene are both potent activators of the complement system, measured both as activated, deposited C3b and quantifying fluid-phase release of the cleavage fragment C3c. The ppVP coated silicone exhibits approximately 90% reduced complement activation compared to untreated silicone. Quartz crystal microbalance with dissipation (QCM-D) measurements show relatively strong adsorption of blood proteins including native C3 to the ppVP surface, indicating that reduction of complement activation on ppVP is neither a result of low protein adsorption nor lower direct C3-binding, and is therefore possibly a consequence of differences in the adsorbed protein layer composition. The alternative and classical complement pathways are barely detectable on ppVP while the lectin pathway through MBL/ficolin-2 deposition remains active on ppVP suggesting this pathway is responsible for the remaining subtle activation on the ppVP coated surface. The ppVP surface is furthermore characterized physically and chemically using scanning electron microscopy (SEM), x-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR), which indicates preservation of chemical functionality by the applied plasma process. Overall, the ppVP coating shows a potential for increasing complement-compatibility of blood-contacting devices.

KW - Adsorption

KW - Coated Materials, Biocompatible

KW - Complement Activation

KW - Complement System Proteins

KW - Humans

KW - Materials Testing

KW - Polymers

KW - Polystyrenes

KW - Pyrrolidines

KW - Silicone Elastomers

KW - Spectroscopy, Fourier Transform Infrared

KW - Surface Properties

KW - Vinyl Compounds

KW - Evaluation Studies

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.biomaterials.2011.03.002

DO - 10.1016/j.biomaterials.2011.03.002

M3 - Journal article

C2 - 21453967

SN - 0142-9612

VL - 32

SP - 4481

EP - 4488

JO - Biomaterials

JF - Biomaterials

IS - 20

ER -