Decreased demand for olfactory periglomerular cells impacts on neural precursor cell viability in the rostral migratory stream

TY - JOUR

T1 - Decreased demand for olfactory periglomerular cells impacts on neural precursor cell viability in the rostral migratory stream

AU - Langenfurth, Anika

AU - Gu, Song

AU - Bautze, Verena

AU - Zhang, Caiyi

AU - Neumann, Julia E

AU - Schüller, Ulrich

AU - Stock, Kristin

AU - Wolf, Susanne A

AU - Maier, Anna-Maria

AU - Mastrella, Giorgia

AU - Pak, Andrew

AU - Cheng, Hongwei

AU - Kälin, Roland E

AU - Holmbeck, Kenn

AU - Strotmann, Jörg

AU - Kettenmann, Helmut

AU - Glass, Rainer

PY - 2016/8/30

Y1 - 2016/8/30

N2 - The subventricular zone (SVZ) provides a constant supply of new neurons to the olfactory bulb (OB). Different studies have investigated the role of olfactory sensory input to neural precursor cell (NPC) turnover in the SVZ but it was not addressed if a reduced demand specifically for periglomerular neurons impacts on NPC-traits in the rostral migratory stream (RMS). We here report that membrane type-1 matrix metalloproteinase (MT1-MMP) deficient mice have reduced complexity of the nasal turbinates, decreased sensory innervation of the OB, reduced numbers of olfactory glomeruli and reduced OB-size without alterations in SVZ neurogenesis. Large parts of the RMS were fully preserved in MT1-MMP-deficient mice, but we detected an increase in cell death-levels and a decrease in SVZ-derived neuroblasts in the distal RMS, as compared to controls. BrdU-tracking experiments showed that homing of NPCs specifically to the glomerular layer was reduced in MT1-MMP-deficient mice in contrast to controls while numbers of tracked cells remained equal in other OB-layers throughout all experimental groups. Altogether, our data show the demand for olfactory interneurons in the glomerular layer modulates cell turnover in the RMS, but has no impact on subventricular neurogenesis.

AB - The subventricular zone (SVZ) provides a constant supply of new neurons to the olfactory bulb (OB). Different studies have investigated the role of olfactory sensory input to neural precursor cell (NPC) turnover in the SVZ but it was not addressed if a reduced demand specifically for periglomerular neurons impacts on NPC-traits in the rostral migratory stream (RMS). We here report that membrane type-1 matrix metalloproteinase (MT1-MMP) deficient mice have reduced complexity of the nasal turbinates, decreased sensory innervation of the OB, reduced numbers of olfactory glomeruli and reduced OB-size without alterations in SVZ neurogenesis. Large parts of the RMS were fully preserved in MT1-MMP-deficient mice, but we detected an increase in cell death-levels and a decrease in SVZ-derived neuroblasts in the distal RMS, as compared to controls. BrdU-tracking experiments showed that homing of NPCs specifically to the glomerular layer was reduced in MT1-MMP-deficient mice in contrast to controls while numbers of tracked cells remained equal in other OB-layers throughout all experimental groups. Altogether, our data show the demand for olfactory interneurons in the glomerular layer modulates cell turnover in the RMS, but has no impact on subventricular neurogenesis.

KW - Animals

KW - Cell Movement/physiology

KW - Cell Survival/physiology

KW - Lateral Ventricles/cytology

KW - Matrix Metalloproteinase 14/genetics

KW - Mice

KW - Mice, Transgenic

KW - Neural Stem Cells/cytology

KW - Olfactory Bulb/cytology

KW - Olfactory Receptor Neurons/cytology

U2 - 10.1038/srep32203

DO - 10.1038/srep32203

M3 - Journal article

C2 - 27573347

SN - 2045-2322

VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 32203

ER -