DcE2F, a functional plant E2F-like transcriptional activator from Daucus carota.

TY - JOUR

T1 - DcE2F, a functional plant E2F-like transcriptional activator from Daucus carota.

AU - Albani, D

AU - Mariconti, L

AU - Ricagno, S

AU - Pitto, L

AU - Moroni, C

AU - Helin, K

AU - Cella, R

N1 - Keywords: Amino Acid Sequence; Animals; Base Sequence; Carrier Proteins; Cell Cycle; Cell Cycle Proteins; DNA, Complementary; DNA-Binding Proteins; Daucus carota; E2F Transcription Factors; Molecular Sequence Data; Sequence Homology, Amino Acid; Trans-Activators; Transcription Factors

PY - 2000

Y1 - 2000

N2 - In animal cells the progression of the cell cycle through G(1)/S transition and S phase is under the control of the pRB/E2F regulatory pathway. The E2F transcription factors are key activators of genes coding for several regulatory proteins and for enzymes involved in nucleotide and DNA synthesis. In this report we have detected the presence of E2F-like DNA binding activities in carrot nuclear extracts, and we have isolated a carrot cDNA (DcE2F) encoding a plant E2F homologue. The DcE2F gene is expressed in proliferating cells and is induced during the G(1)/S transition of the cell cycle. Supershift experiments using anti-DcE2F antiserum have confirmed that the DcE2F protein is a component of the carrot E2F-like nuclear activities. DNA binding assays have demonstrated that the DcE2F protein can recognize a canonical E2F cis-element in association with a mammalian DP protein. Furthermore, transactivation assays have revealed that DcE2F is a functional transcription factor that can transactivate, together with a DP partner, an E2F-responsive reporter gene in both plant and mammalian cells.

AB - In animal cells the progression of the cell cycle through G(1)/S transition and S phase is under the control of the pRB/E2F regulatory pathway. The E2F transcription factors are key activators of genes coding for several regulatory proteins and for enzymes involved in nucleotide and DNA synthesis. In this report we have detected the presence of E2F-like DNA binding activities in carrot nuclear extracts, and we have isolated a carrot cDNA (DcE2F) encoding a plant E2F homologue. The DcE2F gene is expressed in proliferating cells and is induced during the G(1)/S transition of the cell cycle. Supershift experiments using anti-DcE2F antiserum have confirmed that the DcE2F protein is a component of the carrot E2F-like nuclear activities. DNA binding assays have demonstrated that the DcE2F protein can recognize a canonical E2F cis-element in association with a mammalian DP protein. Furthermore, transactivation assays have revealed that DcE2F is a functional transcription factor that can transactivate, together with a DP partner, an E2F-responsive reporter gene in both plant and mammalian cells.

U2 - 10.1074/jbc.M909390199

DO - 10.1074/jbc.M909390199

M3 - Journal article

C2 - 10748129

SN - 0021-9258

VL - 275

SP - 19258

EP - 19267

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 25

ER -