Danish apple cultivars: genetic diversity, parentage and breeding potential

Bjarne Larsen; Carsten Pedersen; Marian Ørgaard; Torben Toldam-Andersen

doi:10.17660/ActaHortic.2017.1172.66

Danish apple cultivars: genetic diversity, parentage and breeding potential

Bjarne Larsen, Carsten Pedersen, Marian Ørgaard, Torben Toldam-Andersen

Abstract

We made a genetic analysis of about 500 apple cultivars, which either originate from Denmark or have been of greater importance in Denmark. To study the diversity and parentage among the cultivars, 15 SSR markers were used. A new PCR-based protocol for identification of S-alleles was developed and used to study the S-alleles among cultivars. The protocol using general S-locus primers and digestion with two restriction enzymes, for fragment analysis on an automated DNA sequencer fastens the process and gives an accurate estimation of fragment lengths. The ploidy level was determined using flow-cytometry. All cultivars were distinguishable by the SSRmarkers though Danish originating cultivars did not form a group distinct from foreign cultivars. The parentage of several well-known cultivars was revealed.

Original language	English
Title of host publication	Proceedings of the XIV EUCARPIA Symposium on Fruit Breeding and Genetics
Number of pages	4
Publisher	International Society for Horticultural Science
Publication date	17 Oct 2017
Pages	355-358
DOIs	https://doi.org/10.17660/ActaHortic.2017.1172.66
Publication status	Published - 17 Oct 2017
Event	Eucarpia Symposium on Fruit Breeding and Genetics 2015 - Bologna, Italy Duration: 14 Jun 2015 → 18 Jun 2015 Conference number: 14 http://www.ishs.org/symposium/382

Conference

Conference	Eucarpia Symposium on Fruit Breeding and Genetics 2015
Number	14
Country/Territory	Italy
City	Bologna
Period	14/06/2015 → 18/06/2015
Internet address	http://www.ishs.org/symposium/382

Series	Acta Horticulturae
Volume	1172
ISSN	0567-7572

Keywords

Apples

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10.17660/ActaHortic.2017.1172.66

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Danish apple cultivars: genetic diversity, parentage and breeding potential. / Larsen, Bjarne ; Pedersen, Carsten ; Ørgaard, Marian et al.
Proceedings of the XIV EUCARPIA Symposium on Fruit Breeding and Genetics . International Society for Horticultural Science, 2017. p. 355-358 (Acta Horticulturae, Vol. 1172).

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Larsen, B , Pedersen, C , Ørgaard, M & Toldam-Andersen, T 2017, Danish apple cultivars: genetic diversity, parentage and breeding potential. in Proceedings of the XIV EUCARPIA Symposium on Fruit Breeding and Genetics . International Society for Horticultural Science, Acta Horticulturae, vol. 1172, pp. 355-358, Eucarpia Symposium on Fruit Breeding and Genetics 2015, Bologna, Italy, 14/06/2015. https://doi.org/10.17660/ActaHortic.2017.1172.66

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Danish apple cultivars: genetic diversity, parentage and breeding potential

Abstract

Conference

Keywords

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