Dam methyltransferase is required for stable lysogeny of the Shiga toxin (Stx2)-encoding bacteriophage 933W of enterohemorrhagic Escherichia coli O157:H7

Kenan C Murphy; Jennifer M Ritchie; Matthew K Waldor; Anders Løbner-Olesen; M G Marinus

doi:10.1128/JB.01373-07

Dam methyltransferase is required for stable lysogeny of the Shiga toxin (Stx2)-encoding bacteriophage 933W of enterohemorrhagic Escherichia coli O157:H7

Kenan C Murphy, Jennifer M Ritchie, Matthew K Waldor, Anders Løbner-Olesen, M G Marinus

Functional Genomics

31 Citations (Scopus)

Abstract

Shiga toxin 2 (Stx2), one of the principal virulence factors of enterohemorrhagic Escherichia coli, is encoded by 933W, a lambda-like prophage. 933W prophage induction contributes to Stx2 production, and here, we provide evidence that Dam methyltransferase is essential for maintenance of 933W lysogeny. Our findings are consistent with the idea that the 933W prophage has a relatively low threshold for induction, which may promote Stx2 production during infection.

Original language	English
Journal	Journal of Bacteriology
Volume	190
Issue number	1
Pages (from-to)	438-41
Number of pages	4
ISSN	0021-9193
DOIs	https://doi.org/10.1128/JB.01373-07
Publication status	Published - Jan 2008

Keywords

Coliphages/enzymology
Enterohemorrhagic Escherichia coli/drug effects
Kanamycin/pharmacology
Lysogeny
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
Shiga Toxin 2/genetics
Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics
Virulence

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10.1128/JB.01373-07

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Dam methyltransferase is required for stable lysogeny of the Shiga toxin (Stx2)-encoding bacteriophage 933W of enterohemorrhagic Escherichia coli O157:H7. / Murphy, Kenan C; Ritchie, Jennifer M; Waldor, Matthew K et al.

In: Journal of Bacteriology, Vol. 190, No. 1, 01.2008, p. 438-41.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Dam methyltransferase is required for stable lysogeny of the Shiga toxin (Stx2)-encoding bacteriophage 933W of enterohemorrhagic Escherichia coli O157:H7",

abstract = "Shiga toxin 2 (Stx2), one of the principal virulence factors of enterohemorrhagic Escherichia coli, is encoded by 933W, a lambda-like prophage. 933W prophage induction contributes to Stx2 production, and here, we provide evidence that Dam methyltransferase is essential for maintenance of 933W lysogeny. Our findings are consistent with the idea that the 933W prophage has a relatively low threshold for induction, which may promote Stx2 production during infection.",

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AU - Murphy, Kenan C

AU - Ritchie, Jennifer M

AU - Waldor, Matthew K

AU - Løbner-Olesen, Anders

AU - Marinus, M G

PY - 2008/1

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N2 - Shiga toxin 2 (Stx2), one of the principal virulence factors of enterohemorrhagic Escherichia coli, is encoded by 933W, a lambda-like prophage. 933W prophage induction contributes to Stx2 production, and here, we provide evidence that Dam methyltransferase is essential for maintenance of 933W lysogeny. Our findings are consistent with the idea that the 933W prophage has a relatively low threshold for induction, which may promote Stx2 production during infection.

AB - Shiga toxin 2 (Stx2), one of the principal virulence factors of enterohemorrhagic Escherichia coli, is encoded by 933W, a lambda-like prophage. 933W prophage induction contributes to Stx2 production, and here, we provide evidence that Dam methyltransferase is essential for maintenance of 933W lysogeny. Our findings are consistent with the idea that the 933W prophage has a relatively low threshold for induction, which may promote Stx2 production during infection.

KW - Coliphages/enzymology

KW - Enterohemorrhagic Escherichia coli/drug effects

KW - Kanamycin/pharmacology

KW - Lysogeny

KW - Oligonucleotide Array Sequence Analysis

KW - Polymerase Chain Reaction

KW - Shiga Toxin 2/genetics

KW - Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics

KW - Virulence

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SN - 0021-9193

VL - 190

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JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 1

ER -

Dam methyltransferase is required for stable lysogeny of the Shiga toxin (Stx2)-encoding bacteriophage 933W of enterohemorrhagic Escherichia coli O157:H7

Abstract

Keywords

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