Cytoprotective effect of tocopherols in hepatocytes cultured with polyunsaturated fatty acids

L. Mikkelsen; Harald S. Hansen; N. Grunnet; J. Dich

Cytoprotective effect of tocopherols in hepatocytes cultured with polyunsaturated fatty acids

L. Mikkelsen, Harald S. Hansen, N. Grunnet, J. Dich

Inflammation, Metabolism and Oxidation

15 Citations (Scopus)

Abstract

When highly unsaturated fatty acids are added to cell cultures, it can become important to include antioxidants in the culture medium to prevent cytotoxic peroxidation. To find an optimal antioxidant for this purpose, the effect of 50 µM a-tocopherol, ¿-tocopherol, a-tocopheryl acetate, a- tocopheryl acid succinate, or a-tocopheryl phosphate, or of 1 µM N,N'- diphenyl-1,4-phenylenediamine, was investigated with respect to the agent's ability to prevent lactate dehydrogenase leakage in long-term rat hepatocyte cultures supplemented with 0.5 mM highly unsaturated fatty acids. Formation of thiobarbituric acid reactive substances in the cultures was also measured. a-Tocopheryl acid succinate was found to be the most effective cytoprotective compound, followed by N,N'-diphenyl-1,4-phenylenediamine, a- tocopherol, ¿-tocopherol and a-tocopheryl acetate, and a-tocopheryl phosphate was without effect.

Original language	English
Journal	Lipids
Volume	29
Issue number	5
Pages (from-to)	369-372
Number of pages	4
ISSN	0024-4201
Publication status	Published - 1994

Cite this

@article{689853e074ce11dbbee902004c4f4f50,

title = "Cytoprotective effect of tocopherols in hepatocytes cultured with polyunsaturated fatty acids",

abstract = "When highly unsaturated fatty acids are added to cell cultures, it can become important to include antioxidants in the culture medium to prevent cytotoxic peroxidation. To find an optimal antioxidant for this purpose, the effect of 50 µM a-tocopherol, ¿-tocopherol, a-tocopheryl acetate, a- tocopheryl acid succinate, or a-tocopheryl phosphate, or of 1 µM N,N'- diphenyl-1,4-phenylenediamine, was investigated with respect to the agent's ability to prevent lactate dehydrogenase leakage in long-term rat hepatocyte cultures supplemented with 0.5 mM highly unsaturated fatty acids. Formation of thiobarbituric acid reactive substances in the cultures was also measured. a-Tocopheryl acid succinate was found to be the most effective cytoprotective compound, followed by N,N'-diphenyl-1,4-phenylenediamine, a- tocopherol, ¿-tocopherol and a-tocopheryl acetate, and a-tocopheryl phosphate was without effect.",

author = "L. Mikkelsen and Hansen, {Harald S.} and N. Grunnet and J. Dich",

year = "1994",

language = "English",

volume = "29",

pages = "369--372",

journal = "Lipids",

issn = "0024-4201",

publisher = "Springer",

number = "5",

}

TY - JOUR

T1 - Cytoprotective effect of tocopherols in hepatocytes cultured with polyunsaturated fatty acids

AU - Mikkelsen, L.

AU - Hansen, Harald S.

AU - Grunnet, N.

AU - Dich, J.

PY - 1994

Y1 - 1994

N2 - When highly unsaturated fatty acids are added to cell cultures, it can become important to include antioxidants in the culture medium to prevent cytotoxic peroxidation. To find an optimal antioxidant for this purpose, the effect of 50 µM a-tocopherol, ¿-tocopherol, a-tocopheryl acetate, a- tocopheryl acid succinate, or a-tocopheryl phosphate, or of 1 µM N,N'- diphenyl-1,4-phenylenediamine, was investigated with respect to the agent's ability to prevent lactate dehydrogenase leakage in long-term rat hepatocyte cultures supplemented with 0.5 mM highly unsaturated fatty acids. Formation of thiobarbituric acid reactive substances in the cultures was also measured. a-Tocopheryl acid succinate was found to be the most effective cytoprotective compound, followed by N,N'-diphenyl-1,4-phenylenediamine, a- tocopherol, ¿-tocopherol and a-tocopheryl acetate, and a-tocopheryl phosphate was without effect.

AB - When highly unsaturated fatty acids are added to cell cultures, it can become important to include antioxidants in the culture medium to prevent cytotoxic peroxidation. To find an optimal antioxidant for this purpose, the effect of 50 µM a-tocopherol, ¿-tocopherol, a-tocopheryl acetate, a- tocopheryl acid succinate, or a-tocopheryl phosphate, or of 1 µM N,N'- diphenyl-1,4-phenylenediamine, was investigated with respect to the agent's ability to prevent lactate dehydrogenase leakage in long-term rat hepatocyte cultures supplemented with 0.5 mM highly unsaturated fatty acids. Formation of thiobarbituric acid reactive substances in the cultures was also measured. a-Tocopheryl acid succinate was found to be the most effective cytoprotective compound, followed by N,N'-diphenyl-1,4-phenylenediamine, a- tocopherol, ¿-tocopherol and a-tocopheryl acetate, and a-tocopheryl phosphate was without effect.

UR - http://www.scopus.com/inward/record.url?scp=0028270523&partnerID=8YFLogxK

M3 - Journal article

AN - SCOPUS:0028270523

SN - 0024-4201

VL - 29

SP - 369

EP - 372

JO - Lipids

JF - Lipids

IS - 5

ER -

Cytoprotective effect of tocopherols in hepatocytes cultured with polyunsaturated fatty acids

Abstract

Other files and links

Fingerprint

Cite this