TY - JOUR
T1 - Cytochrome P450-mediated metabolic engineering
T2 - current progress and future challenges
AU - Renault, Hugues
AU - Bassard, Jean-Étienne André
AU - Hamberger, Björn Robert
AU - Werck-Reichhart, Danièle
N1 - SI: Physiology and metabolism
PY - 2014
Y1 - 2014
N2 - Cytochromes P450 catalyze a broad range of regiospecific, stereospecific and irreversible steps in the biosynthetic routes of plant natural metabolites with important applications in pharmaceutical, cosmetic, fragrance and flavour, or polymer industries. They are consequently essential drivers for the engineered bioproduction of such compounds. Two ground-breaking developments of commercial products driven by the engineering of P450s are the antimalarial drug precursor artemisinic acid and blue roses or carnations. Tedious optimizations were required to generate marketable products. Hurdles encountered in P450 engineering and their potential solutions are summarized here. Together with recent technical developments and novel approaches to metabolic engineering, the lessons from this pioneering work should considerably boost exploitation of the amazing P450 toolkit emerging from accelerated sequencing of plant genomes.
AB - Cytochromes P450 catalyze a broad range of regiospecific, stereospecific and irreversible steps in the biosynthetic routes of plant natural metabolites with important applications in pharmaceutical, cosmetic, fragrance and flavour, or polymer industries. They are consequently essential drivers for the engineered bioproduction of such compounds. Two ground-breaking developments of commercial products driven by the engineering of P450s are the antimalarial drug precursor artemisinic acid and blue roses or carnations. Tedious optimizations were required to generate marketable products. Hurdles encountered in P450 engineering and their potential solutions are summarized here. Together with recent technical developments and novel approaches to metabolic engineering, the lessons from this pioneering work should considerably boost exploitation of the amazing P450 toolkit emerging from accelerated sequencing of plant genomes.
U2 - 10.1016/j.pbi.2014.03.004
DO - 10.1016/j.pbi.2014.03.004
M3 - Journal article
C2 - 24709279
AN - SCOPUS:84897553187
SN - 1369-5266
VL - 19
SP - 27
EP - 34
JO - Current Opinion in Plant Biology
JF - Current Opinion in Plant Biology
ER -