Current and emerging approaches to define intestinal epithelium-specific transcriptional networks

TY - JOUR

T1 - Current and emerging approaches to define intestinal epithelium-specific transcriptional networks

AU - Olsen, Anders Krüger

AU - Boyd, Mette

AU - Danielsen, Erik Thomas

AU - Troelsen, Jesper Thorvald

PY - 2012/2

Y1 - 2012/2

N2 - Upon developmental or environmental cues, the composition of transcription factors in a transcriptional regulatory network is deeply implicated in controlling the signature of the gene expression and thereby specifies the cell or tissue type. Novel methods including ChIP-chip and ChIP-Seq have been applied to analyze known transcription factors and their interacting regulatory DNA elements in the intestine. The intestine is an example of a dynamic tissue where stem cells in the crypt proliferate and undergo a differentiation process toward the villus. During this differentiation process, specific regulatory networks of transcription factors are activated to target specific genes, which determine the intestinal cell fate. The expanding genomewide mapping of transcription factor binding sites and construction of transcriptional regulatory networks provide new insight into how intestinal differentiation occurs. This review summarizes the current overview of the transcriptional regulatory networks driving epithelial differentiation in adult intestine. The novel technologies that have been implied to study these networks are presented and their prospects for implications in future research are also addressed.

AB - Upon developmental or environmental cues, the composition of transcription factors in a transcriptional regulatory network is deeply implicated in controlling the signature of the gene expression and thereby specifies the cell or tissue type. Novel methods including ChIP-chip and ChIP-Seq have been applied to analyze known transcription factors and their interacting regulatory DNA elements in the intestine. The intestine is an example of a dynamic tissue where stem cells in the crypt proliferate and undergo a differentiation process toward the villus. During this differentiation process, specific regulatory networks of transcription factors are activated to target specific genes, which determine the intestinal cell fate. The expanding genomewide mapping of transcription factor binding sites and construction of transcriptional regulatory networks provide new insight into how intestinal differentiation occurs. This review summarizes the current overview of the transcriptional regulatory networks driving epithelial differentiation in adult intestine. The novel technologies that have been implied to study these networks are presented and their prospects for implications in future research are also addressed.

KW - Animals

KW - Cell Differentiation

KW - Chromatin Immunoprecipitation

KW - Gene Regulatory Networks

KW - Humans

KW - Intestinal Mucosa

KW - Organ Specificity

KW - Transcription Factors

U2 - 10.1152/ajpgi.00362.2011

DO - 10.1152/ajpgi.00362.2011

M3 - Journal article

C2 - 22094602

SN - 0193-1857

VL - 302

SP - G277-86

JO - American Journal of Physiology: Gastrointestinal and Liver Physiology

JF - American Journal of Physiology: Gastrointestinal and Liver Physiology

IS - 3

ER -