Crystallization of the HigBA2 toxin-antitoxin complex from Vibrio cholerae

TY - JOUR

T1 - Crystallization of the HigBA2 toxin-antitoxin complex from Vibrio cholerae

AU - Hadzi, San

AU - Garcia-Pino, Abel

AU - Martinez-Rodriguez, Sergio

AU - Verschueren, Koen

AU - Christensen-Dalsgaard, Mikkel

AU - Gerdes, Kenn

AU - Lah, Jurij

AU - Loris, Remy

PY - 2013/9

Y1 - 2013/9

N2 - The genome of Vibrio cholerae encodes two higBA toxin-antitoxin (TA) modules that are activated by amino-acid starvation. Here, the TA complex of the second module, higBA2, as well as the C-terminal domain of the corresponding HigA2 antitoxin, have been purified and crystallized. The HigBA2 complex crystallized in two crystal forms. Crystals of form I belonged to space group P21212, with unit-cell parameters a = 129.0, b = 119.8, c = 33.4Å, and diffracted to 3.0Å resolution. The asymmetric unit is likely to contain a single complex consisting of two toxin monomers and one antitoxin dimer. The second crystal form crystallized in space group P3221, with unit-cell parameters a = 134.5, c = 55.4Å. These crystals diffracted to 2.2Å resolution and probably contain a complex with a different stoichiometry. Crystals of the C-terminal domain of HigA2 belonged to space group C2, with unit-cell parameters a = 115.4, b = 61.2, c = 73.8Å, β = 106.7°, and diffracted to 1.8Å resolution.

AB - The genome of Vibrio cholerae encodes two higBA toxin-antitoxin (TA) modules that are activated by amino-acid starvation. Here, the TA complex of the second module, higBA2, as well as the C-terminal domain of the corresponding HigA2 antitoxin, have been purified and crystallized. The HigBA2 complex crystallized in two crystal forms. Crystals of form I belonged to space group P21212, with unit-cell parameters a = 129.0, b = 119.8, c = 33.4Å, and diffracted to 3.0Å resolution. The asymmetric unit is likely to contain a single complex consisting of two toxin monomers and one antitoxin dimer. The second crystal form crystallized in space group P3221, with unit-cell parameters a = 134.5, c = 55.4Å. These crystals diffracted to 2.2Å resolution and probably contain a complex with a different stoichiometry. Crystals of the C-terminal domain of HigA2 belonged to space group C2, with unit-cell parameters a = 115.4, b = 61.2, c = 73.8Å, β = 106.7°, and diffracted to 1.8Å resolution.

U2 - 10.1107/S1744309113021490

DO - 10.1107/S1744309113021490

M3 - Journal article

C2 - 23989162

SN - 2053-230X

VL - 69

SP - 1052

EP - 1059

JO - Acta Crystallographica Section F: Structural Biology Communications

JF - Acta Crystallographica Section F: Structural Biology Communications

IS - 9

ER -