CRISPR-based immune systems of the Sulfolobales: complexity and diversity

Roger Antony Garrett; Shiraz Ali Shah; Gisle Alberg Vestergaard; Ling Deng; Sóley Ruth Guðbergsdóttir; Chandra Shekar Kenchappa; Susanne Erdmann; Qunxin She

doi:10.1042/BST0390051

CRISPR-based immune systems of the Sulfolobales: complexity and diversity

Roger Antony Garrett, Shiraz Ali Shah, Gisle Alberg Vestergaard, Ling Deng, Sóley Ruth Guðbergsdóttir, Chandra Shekar Kenchappa, Susanne Erdmann, Qunxin She

Functional Genomics

48 Citations (Scopus)

Abstract

CRISPR (cluster of regularly interspaced palindromic repeats)/Cas and CRISPR/Cmr systems of Sulfolobus, targeting DNA and RNA respectively of invading viruses or plasmids are complex and diverse. We address their classification and functional diversity, and the wide sequence diversity of RAMP (repeat-associated mysterious protein)-motif containing proteins encoded in Cmr modules. Factors influencing maintenance of partially impaired CRISPR-based systems are discussed. The capacity for whole CRISPR transcripts to be generated despite the uptake of transcription signals within spacer sequences is considered. Targeting of protospacer regions of invading elements by Cas protein-crRNA (CRISPR RNA) complexes exhibit relatively low sequence stringency, but the integrity of protospacer-associated motifs appears to be important. Different mechanisms for circumventing or inactivating the immune systems are presented.

Original language	English
Journal	Biochemical Society Transactions
Volume	39
Issue number	1
Pages (from-to)	51-57
Number of pages	7
ISSN	1470-8752
DOIs	https://doi.org/10.1042/BST0390051
Publication status	Published - Feb 2011

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abstract = "CRISPR (cluster of regularly interspaced palindromic repeats)/Cas and CRISPR/Cmr systems of Sulfolobus, targeting DNA and RNA respectively of invading viruses or plasmids are complex and diverse. We address their classification and functional diversity, and the wide sequence diversity of RAMP (repeat-associated mysterious protein)-motif containing proteins encoded in Cmr modules. Factors influencing maintenance of partially impaired CRISPR-based systems are discussed. The capacity for whole CRISPR transcripts to be generated despite the uptake of transcription signals within spacer sequences is considered. Targeting of protospacer regions of invading elements by Cas protein-crRNA (CRISPR RNA) complexes exhibit relatively low sequence stringency, but the integrity of protospacer-associated motifs appears to be important. Different mechanisms for circumventing or inactivating the immune systems are presented.",

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AU - Garrett, Roger Antony

AU - Shah, Shiraz Ali

AU - Vestergaard, Gisle Alberg

AU - Deng, Ling

AU - Guðbergsdóttir, Sóley Ruth

AU - Kenchappa, Chandra Shekar

AU - Erdmann, Susanne

AU - She, Qunxin

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AB - CRISPR (cluster of regularly interspaced palindromic repeats)/Cas and CRISPR/Cmr systems of Sulfolobus, targeting DNA and RNA respectively of invading viruses or plasmids are complex and diverse. We address their classification and functional diversity, and the wide sequence diversity of RAMP (repeat-associated mysterious protein)-motif containing proteins encoded in Cmr modules. Factors influencing maintenance of partially impaired CRISPR-based systems are discussed. The capacity for whole CRISPR transcripts to be generated despite the uptake of transcription signals within spacer sequences is considered. Targeting of protospacer regions of invading elements by Cas protein-crRNA (CRISPR RNA) complexes exhibit relatively low sequence stringency, but the integrity of protospacer-associated motifs appears to be important. Different mechanisms for circumventing or inactivating the immune systems are presented.

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DO - 10.1042/BST0390051

M3 - Journal article

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JO - Biochemical Society Transactions

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CRISPR-based immune systems of the Sulfolobales: complexity and diversity

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