Coupling biogeochemical process rates and metagenomic blueprints of coastal bacterial assemblages in the context of environmental change

Trine Marenda Markussen; Elisabeth Münster Happel; Jonna E. Teikari; Vimala Huchaiah; Johannes Alneberg; Anders F. Andersson; Kaarina Sivonen; Lasse Riemann; Mathias Middelboe; Veljo Kisand

doi:10.1111/1462-2920.14371

Coupling biogeochemical process rates and metagenomic blueprints of coastal bacterial assemblages in the context of environmental change

Trine Marenda Markussen, Elisabeth Münster Happel, Jonna E. Teikari, Vimala Huchaiah, Johannes Alneberg, Anders F. Andersson, Kaarina Sivonen, Lasse Riemann, Mathias Middelboe, Veljo Kisand

Marine Biology

3 Citations (Scopus)

Abstract

Bacteria are major drivers of biogeochemical nutrient cycles and energy fluxes in marine environments, yet how bacterial communities respond to environmental change is not well known. Metagenomes allow examination of genetic responses of the entire microbial community to environmental change. However, it is challenging to link metagenomes directly to biogeochemical process rates. Here, we investigate metagenomic responses in natural bacterioplankton communities to simulated environmental stressors in the Baltic Sea, including increased river water input, increased nutrient concentration, and reduced oxygen level. This allowed us to identify informative prokaryotic gene markers, responding to environmental perturbation. Our results demonstrate that metagenomic and metabolic changes in bacterial communities in response to environmental stressors are influenced both by the initial community composition and by the biogeochemical factors shaping the functional response. Furthermore, the different sources of dissolved organic matter (DOM) had the largest impact on metagenomic blueprint. Most prominently, changes in DOM loads influenced specific transporter types reflecting the substrate availability and DOC assimilation and consumption pathways. The results provide new knowledge for developing models of ecosystem structure and biogeochemical cycling in future climate change scenarios and advance our exploration of the potential use of marine microorganisms as markers for environmental conditions.

Original language	English
Journal	Environmental Microbiology
Volume	20
Issue number	8
Pages (from-to)	3083-3099
ISSN	1462-2912
DOIs	https://doi.org/10.1111/1462-2920.14371
Publication status	Published - Aug 2018

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Markussen, T. M., Happel, E. M., Teikari, J. E., Huchaiah, V., Alneberg, J., Andersson, A. F., Sivonen, K., Riemann, L., Middelboe, M., & Kisand, V. (2018). Coupling biogeochemical process rates and metagenomic blueprints of coastal bacterial assemblages in the context of environmental change. Environmental Microbiology, 20(8), 3083-3099. https://doi.org/10.1111/1462-2920.14371

Coupling biogeochemical process rates and metagenomic blueprints of coastal bacterial assemblages in the context of environmental change. / Markussen, Trine Marenda; Happel, Elisabeth Münster; Teikari, Jonna E. et al.
In: Environmental Microbiology, Vol. 20, No. 8, 08.2018, p. 3083-3099.

Research output: Contribution to journal › Journal article › Research › peer-review

Markussen, TM, Happel, EM, Teikari, JE, Huchaiah, V, Alneberg, J, Andersson, AF, Sivonen, K, Riemann, L , Middelboe, M & Kisand, V 2018, 'Coupling biogeochemical process rates and metagenomic blueprints of coastal bacterial assemblages in the context of environmental change', Environmental Microbiology, vol. 20, no. 8, pp. 3083-3099. https://doi.org/10.1111/1462-2920.14371

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title = "Coupling biogeochemical process rates and metagenomic blueprints of coastal bacterial assemblages in the context of environmental change",

abstract = "Bacteria are major drivers of biogeochemical nutrient cycles and energy fluxes in marine environments, yet how bacterial communities respond to environmental change is not well known. Metagenomes allow examination of genetic responses of the entire microbial community to environmental change. However, it is challenging to link metagenomes directly to biogeochemical process rates. Here, we investigate metagenomic responses in natural bacterioplankton communities to simulated environmental stressors in the Baltic Sea, including increased river water input, increased nutrient concentration, and reduced oxygen level. This allowed us to identify informative prokaryotic gene markers, responding to environmental perturbation. Our results demonstrate that metagenomic and metabolic changes in bacterial communities in response to environmental stressors are influenced both by the initial community composition and by the biogeochemical factors shaping the functional response. Furthermore, the different sources of dissolved organic matter (DOM) had the largest impact on metagenomic blueprint. Most prominently, changes in DOM loads influenced specific transporter types reflecting the substrate availability and DOC assimilation and consumption pathways. The results provide new knowledge for developing models of ecosystem structure and biogeochemical cycling in future climate change scenarios and advance our exploration of the potential use of marine microorganisms as markers for environmental conditions.",

author = "Markussen, {Trine Marenda} and Happel, {Elisabeth M{\"u}nster} and Teikari, {Jonna E.} and Vimala Huchaiah and Johannes Alneberg and Andersson, {Anders F.} and Kaarina Sivonen and Lasse Riemann and Mathias Middelboe and Veljo Kisand",

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AU - Markussen, Trine Marenda

AU - Happel, Elisabeth Münster

AU - Teikari, Jonna E.

AU - Huchaiah, Vimala

AU - Alneberg, Johannes

AU - Andersson, Anders F.

AU - Sivonen, Kaarina

AU - Riemann, Lasse

AU - Middelboe, Mathias

AU - Kisand, Veljo

PY - 2018/8

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N2 - Bacteria are major drivers of biogeochemical nutrient cycles and energy fluxes in marine environments, yet how bacterial communities respond to environmental change is not well known. Metagenomes allow examination of genetic responses of the entire microbial community to environmental change. However, it is challenging to link metagenomes directly to biogeochemical process rates. Here, we investigate metagenomic responses in natural bacterioplankton communities to simulated environmental stressors in the Baltic Sea, including increased river water input, increased nutrient concentration, and reduced oxygen level. This allowed us to identify informative prokaryotic gene markers, responding to environmental perturbation. Our results demonstrate that metagenomic and metabolic changes in bacterial communities in response to environmental stressors are influenced both by the initial community composition and by the biogeochemical factors shaping the functional response. Furthermore, the different sources of dissolved organic matter (DOM) had the largest impact on metagenomic blueprint. Most prominently, changes in DOM loads influenced specific transporter types reflecting the substrate availability and DOC assimilation and consumption pathways. The results provide new knowledge for developing models of ecosystem structure and biogeochemical cycling in future climate change scenarios and advance our exploration of the potential use of marine microorganisms as markers for environmental conditions.

AB - Bacteria are major drivers of biogeochemical nutrient cycles and energy fluxes in marine environments, yet how bacterial communities respond to environmental change is not well known. Metagenomes allow examination of genetic responses of the entire microbial community to environmental change. However, it is challenging to link metagenomes directly to biogeochemical process rates. Here, we investigate metagenomic responses in natural bacterioplankton communities to simulated environmental stressors in the Baltic Sea, including increased river water input, increased nutrient concentration, and reduced oxygen level. This allowed us to identify informative prokaryotic gene markers, responding to environmental perturbation. Our results demonstrate that metagenomic and metabolic changes in bacterial communities in response to environmental stressors are influenced both by the initial community composition and by the biogeochemical factors shaping the functional response. Furthermore, the different sources of dissolved organic matter (DOM) had the largest impact on metagenomic blueprint. Most prominently, changes in DOM loads influenced specific transporter types reflecting the substrate availability and DOC assimilation and consumption pathways. The results provide new knowledge for developing models of ecosystem structure and biogeochemical cycling in future climate change scenarios and advance our exploration of the potential use of marine microorganisms as markers for environmental conditions.

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DO - 10.1111/1462-2920.14371

M3 - Journal article

C2 - 30084235

SN - 1462-2912

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EP - 3099

JO - Environmental Microbiology

JF - Environmental Microbiology

IS - 8

ER -

Coupling biogeochemical process rates and metagenomic blueprints of coastal bacterial assemblages in the context of environmental change

Abstract

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