Correlation between HPV status at T and N sites of oropharyngeal squamous cell carcinomas

Michael Vallop Josiassen; Birgitte Charabi; Christel Braemer Lajer; Emilie Garnaes; Marianne Hamilton Therkildsen; Bodil Norrild; Lena Specht; Finn Cilius Nielsen; Christian von Buchwald

doi:10.1080/00016489.2017.1356934

Correlation between HPV status at T and N sites of oropharyngeal squamous cell carcinomas

Michael Vallop Josiassen^*, Birgitte Charabi, Christel Braemer Lajer, Emilie Garnaes, Marianne Hamilton Therkildsen, Bodil Norrild, Lena Specht, Finn Cilius Nielsen, Christian von Buchwald

^*Corresponding author for this work

Department of Clinical Medicine

1 Citation (Scopus)

Abstract

Objectives: Human papilloma virus (HPV) is known to be associated with oropharyngeal squamous cell carcinomas (OPSCC) and may potentially play a vital role in tumor metastasis. The purpose of this study was to correlate HPV status of cervical lymph node metastases with their respective primary OPSCC tumor. Methods: Formalin-fixed, paraffin-embedded (FFPE) tissue samples obtained from 34 patients with cervical lymph node metastases were analyzed with HPV 16 DNA polymerase chain reaction (PCR), p16 immunohistochemistry and HPV typing. The results were correlated with the HPV status and type found in the primary tumors of OPSCC. Results: Comparing HPV DNA status with p16 we found that 21 primary tumors and lymph node metastases were HPV positive (61.8%) and seven primary tumors and lymph node metastases were HPV negative (20.6%). Six patient samples differed when correlating the primary tumor and lymph node metastasis (17.6%). Conclusions: In this study, HPV status in OPSCCs and their cervical lymph node metastases correlated in the vast majority of cases. However, HPV detection methods may have certain limitations resulting in varying degree of non-correlation. This should be taken into account when stratifying treatment in regard to HPV status.

Original language	English
Book series	Acta Oto-Laryngologica
Volume	137
Issue number	12
Pages (from-to)	1260-1264
Number of pages	5
ISSN	0001-6489
DOIs	https://doi.org/10.1080/00016489.2017.1356934
Publication status	Published - Dec 2017

Keywords

cervical lymph node metastases
HPV
oropharyngeal cancer
p16
squamous cell carcinoma

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1080/00016489.2017.1356934

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@article{198b9911e1a549369e67ab08d70af524,

title = "Correlation between HPV status at T and N sites of oropharyngeal squamous cell carcinomas",

abstract = "Objectives: Human papilloma virus (HPV) is known to be associated with oropharyngeal squamous cell carcinomas (OPSCC) and may potentially play a vital role in tumor metastasis. The purpose of this study was to correlate HPV status of cervical lymph node metastases with their respective primary OPSCC tumor. Methods: Formalin-fixed, paraffin-embedded (FFPE) tissue samples obtained from 34 patients with cervical lymph node metastases were analyzed with HPV 16 DNA polymerase chain reaction (PCR), p16 immunohistochemistry and HPV typing. The results were correlated with the HPV status and type found in the primary tumors of OPSCC. Results: Comparing HPV DNA status with p16 we found that 21 primary tumors and lymph node metastases were HPV positive (61.8%) and seven primary tumors and lymph node metastases were HPV negative (20.6%). Six patient samples differed when correlating the primary tumor and lymph node metastasis (17.6%). Conclusions: In this study, HPV status in OPSCCs and their cervical lymph node metastases correlated in the vast majority of cases. However, HPV detection methods may have certain limitations resulting in varying degree of non-correlation. This should be taken into account when stratifying treatment in regard to HPV status.",

keywords = "cervical lymph node metastases, HPV, oropharyngeal cancer, p16, squamous cell carcinoma",

author = "Josiassen, {Michael Vallop} and Birgitte Charabi and Lajer, {Christel Braemer} and Emilie Garnaes and {Hamilton Therkildsen}, Marianne and Bodil Norrild and Lena Specht and Nielsen, {Finn Cilius} and {von Buchwald}, Christian",

year = "2017",

month = dec,

doi = "10.1080/00016489.2017.1356934",

language = "English",

volume = "137",

pages = "1260--1264",

journal = "Acta Oto-Laryngologica",

issn = "0001-6489",

publisher = "Taylor & Francis",

number = "12",

}

TY - JOUR

T1 - Correlation between HPV status at T and N sites of oropharyngeal squamous cell carcinomas

AU - Josiassen, Michael Vallop

AU - Charabi, Birgitte

AU - Lajer, Christel Braemer

AU - Garnaes, Emilie

AU - Hamilton Therkildsen, Marianne

AU - Norrild, Bodil

AU - Specht, Lena

AU - Nielsen, Finn Cilius

AU - von Buchwald, Christian

PY - 2017/12

Y1 - 2017/12

N2 - Objectives: Human papilloma virus (HPV) is known to be associated with oropharyngeal squamous cell carcinomas (OPSCC) and may potentially play a vital role in tumor metastasis. The purpose of this study was to correlate HPV status of cervical lymph node metastases with their respective primary OPSCC tumor. Methods: Formalin-fixed, paraffin-embedded (FFPE) tissue samples obtained from 34 patients with cervical lymph node metastases were analyzed with HPV 16 DNA polymerase chain reaction (PCR), p16 immunohistochemistry and HPV typing. The results were correlated with the HPV status and type found in the primary tumors of OPSCC. Results: Comparing HPV DNA status with p16 we found that 21 primary tumors and lymph node metastases were HPV positive (61.8%) and seven primary tumors and lymph node metastases were HPV negative (20.6%). Six patient samples differed when correlating the primary tumor and lymph node metastasis (17.6%). Conclusions: In this study, HPV status in OPSCCs and their cervical lymph node metastases correlated in the vast majority of cases. However, HPV detection methods may have certain limitations resulting in varying degree of non-correlation. This should be taken into account when stratifying treatment in regard to HPV status.

AB - Objectives: Human papilloma virus (HPV) is known to be associated with oropharyngeal squamous cell carcinomas (OPSCC) and may potentially play a vital role in tumor metastasis. The purpose of this study was to correlate HPV status of cervical lymph node metastases with their respective primary OPSCC tumor. Methods: Formalin-fixed, paraffin-embedded (FFPE) tissue samples obtained from 34 patients with cervical lymph node metastases were analyzed with HPV 16 DNA polymerase chain reaction (PCR), p16 immunohistochemistry and HPV typing. The results were correlated with the HPV status and type found in the primary tumors of OPSCC. Results: Comparing HPV DNA status with p16 we found that 21 primary tumors and lymph node metastases were HPV positive (61.8%) and seven primary tumors and lymph node metastases were HPV negative (20.6%). Six patient samples differed when correlating the primary tumor and lymph node metastasis (17.6%). Conclusions: In this study, HPV status in OPSCCs and their cervical lymph node metastases correlated in the vast majority of cases. However, HPV detection methods may have certain limitations resulting in varying degree of non-correlation. This should be taken into account when stratifying treatment in regard to HPV status.

KW - cervical lymph node metastases

KW - HPV

KW - oropharyngeal cancer

KW - p16

KW - squamous cell carcinoma

U2 - 10.1080/00016489.2017.1356934

DO - 10.1080/00016489.2017.1356934

M3 - Journal article

C2 - 28758552

AN - SCOPUS:85026551127

SN - 0001-6489

VL - 137

SP - 1260

EP - 1264

JO - Acta Oto-Laryngologica

JF - Acta Oto-Laryngologica

IS - 12

ER -

Correlation between HPV status at T and N sites of oropharyngeal squamous cell carcinomas

Abstract

Keywords

UN SDGs

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