Coregulation of glucose uptake and vascular endothelial growth factor (VEGF) in two small-cell lung cancer (SCLC) sublines in vivo and in vitro

M W Pedersen; S Holm; E L Lund; L Højgaard; P E Kristjansen

doi:10.1038/sj/neo/7900133

Coregulation of glucose uptake and vascular endothelial growth factor (VEGF) in two small-cell lung cancer (SCLC) sublines in vivo and in vitro

M W Pedersen, S Holm, E L Lund, L Højgaard, P E Kristjansen

Department of Clinical Medicine

78 Citations (Scopus)

Abstract

We examined the relationship between (18)F- labeled 2-fluro-2-deoxy-d-glucose (FDG) uptake, and expression of glucose transporters (GLUTs) in two human small-cell lung cancer (SCLC) lines CPH 54A and CPH 54B. Changes in the expression of GLUTs and vascular endothelial growth factor (VEGF) during 12-, 18-, and 24 hours of severe hypoxia in vivo (xenografts) and in vitro (cell cultures) were recorded for both tumor lines. The two SCLC lines are subpopulations of the same patient tumor. In spite of their common genomic origin they represent consistently different metabolic and microenvironmental phenotypes as well as treatment sensitivities. There were higher levels of Glut-1 protein in 54B and a correspondingly higher FDG uptake in this tumor line (P<.001). During hypoxia a significant upregulation of in VEGF mRNA, GLUT-1 mRNA, and Glut-1 and -3 protein occurred with a distinctly different time course in the two cell lines. A similar co-upregulation of GLUT and VEGF was seen in hypoxic tumors of both lines. There were no significant changes of HIF-1alpha mRNA during hypoxia in either of the cell lines. A more detailed understanding of such correlations between glucose metabolism, angiogenesis, and microenvironmental phenotype of tumors, by positron emission tomography (PET) and molecular techniques might further sophisticate our interpretation of glycolytic predominance in tumors as seen by 18FFDG PET.

Original language	English
Journal	Neoplasia (New York, N.Y.)
Volume	3
Issue number	1
Pages (from-to)	80-7
Number of pages	8
ISSN	1522-8002
DOIs	https://doi.org/10.1038/sj/neo/7900133
Publication status	Published - 28 Apr 2001

Keywords

Animals
Anoxia
Blotting, Western
Carcinoma, Small Cell
DNA-Binding Proteins
Endothelial Growth Factors
Fluorodeoxyglucose F18
Glucose
Glucose Transporter Type 1
Humans
Hypoxia-Inducible Factor 1
Hypoxia-Inducible Factor 1, alpha Subunit
In Vitro Techniques
Lung Neoplasms
Lymphokines
Male
Mice
Mice, Nude
Monosaccharide Transport Proteins
Nuclear Proteins
RNA, Messenger
Tomography, Emission-Computed
Transcription Factors
Tumor Cells, Cultured
Up-Regulation
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
Journal Article
Research Support, Non-U.S. Gov't

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/sj/neo/7900133

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title = "Coregulation of glucose uptake and vascular endothelial growth factor (VEGF) in two small-cell lung cancer (SCLC) sublines in vivo and in vitro",

abstract = "We examined the relationship between (18)F- labeled 2-fluro-2-deoxy-d-glucose (FDG) uptake, and expression of glucose transporters (GLUTs) in two human small-cell lung cancer (SCLC) lines CPH 54A and CPH 54B. Changes in the expression of GLUTs and vascular endothelial growth factor (VEGF) during 12-, 18-, and 24 hours of severe hypoxia in vivo (xenografts) and in vitro (cell cultures) were recorded for both tumor lines. The two SCLC lines are subpopulations of the same patient tumor. In spite of their common genomic origin they represent consistently different metabolic and microenvironmental phenotypes as well as treatment sensitivities. There were higher levels of Glut-1 protein in 54B and a correspondingly higher FDG uptake in this tumor line (P<.001). During hypoxia a significant upregulation of in VEGF mRNA, GLUT-1 mRNA, and Glut-1 and -3 protein occurred with a distinctly different time course in the two cell lines. A similar co-upregulation of GLUT and VEGF was seen in hypoxic tumors of both lines. There were no significant changes of HIF-1alpha mRNA during hypoxia in either of the cell lines. A more detailed understanding of such correlations between glucose metabolism, angiogenesis, and microenvironmental phenotype of tumors, by positron emission tomography (PET) and molecular techniques might further sophisticate our interpretation of glycolytic predominance in tumors as seen by 18FFDG PET.",

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author = "Pedersen, {M W} and S Holm and Lund, {E L} and L H{\o}jgaard and Kristjansen, {P E}",

year = "2001",

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doi = "10.1038/sj/neo/7900133",

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T1 - Coregulation of glucose uptake and vascular endothelial growth factor (VEGF) in two small-cell lung cancer (SCLC) sublines in vivo and in vitro

AU - Pedersen, M W

AU - Holm, S

AU - Lund, E L

AU - Højgaard, L

AU - Kristjansen, P E

PY - 2001/4/28

Y1 - 2001/4/28

N2 - We examined the relationship between (18)F- labeled 2-fluro-2-deoxy-d-glucose (FDG) uptake, and expression of glucose transporters (GLUTs) in two human small-cell lung cancer (SCLC) lines CPH 54A and CPH 54B. Changes in the expression of GLUTs and vascular endothelial growth factor (VEGF) during 12-, 18-, and 24 hours of severe hypoxia in vivo (xenografts) and in vitro (cell cultures) were recorded for both tumor lines. The two SCLC lines are subpopulations of the same patient tumor. In spite of their common genomic origin they represent consistently different metabolic and microenvironmental phenotypes as well as treatment sensitivities. There were higher levels of Glut-1 protein in 54B and a correspondingly higher FDG uptake in this tumor line (P<.001). During hypoxia a significant upregulation of in VEGF mRNA, GLUT-1 mRNA, and Glut-1 and -3 protein occurred with a distinctly different time course in the two cell lines. A similar co-upregulation of GLUT and VEGF was seen in hypoxic tumors of both lines. There were no significant changes of HIF-1alpha mRNA during hypoxia in either of the cell lines. A more detailed understanding of such correlations between glucose metabolism, angiogenesis, and microenvironmental phenotype of tumors, by positron emission tomography (PET) and molecular techniques might further sophisticate our interpretation of glycolytic predominance in tumors as seen by 18FFDG PET.

AB - We examined the relationship between (18)F- labeled 2-fluro-2-deoxy-d-glucose (FDG) uptake, and expression of glucose transporters (GLUTs) in two human small-cell lung cancer (SCLC) lines CPH 54A and CPH 54B. Changes in the expression of GLUTs and vascular endothelial growth factor (VEGF) during 12-, 18-, and 24 hours of severe hypoxia in vivo (xenografts) and in vitro (cell cultures) were recorded for both tumor lines. The two SCLC lines are subpopulations of the same patient tumor. In spite of their common genomic origin they represent consistently different metabolic and microenvironmental phenotypes as well as treatment sensitivities. There were higher levels of Glut-1 protein in 54B and a correspondingly higher FDG uptake in this tumor line (P<.001). During hypoxia a significant upregulation of in VEGF mRNA, GLUT-1 mRNA, and Glut-1 and -3 protein occurred with a distinctly different time course in the two cell lines. A similar co-upregulation of GLUT and VEGF was seen in hypoxic tumors of both lines. There were no significant changes of HIF-1alpha mRNA during hypoxia in either of the cell lines. A more detailed understanding of such correlations between glucose metabolism, angiogenesis, and microenvironmental phenotype of tumors, by positron emission tomography (PET) and molecular techniques might further sophisticate our interpretation of glycolytic predominance in tumors as seen by 18FFDG PET.

KW - Animals

KW - Anoxia

KW - Blotting, Western

KW - Carcinoma, Small Cell

KW - DNA-Binding Proteins

KW - Endothelial Growth Factors

KW - Fluorodeoxyglucose F18

KW - Glucose

KW - Glucose Transporter Type 1

KW - Humans

KW - Hypoxia-Inducible Factor 1

KW - Hypoxia-Inducible Factor 1, alpha Subunit

KW - In Vitro Techniques

KW - Lung Neoplasms

KW - Lymphokines

KW - Male

KW - Mice

KW - Mice, Nude

KW - Monosaccharide Transport Proteins

KW - Nuclear Proteins

KW - RNA, Messenger

KW - Tomography, Emission-Computed

KW - Transcription Factors

KW - Tumor Cells, Cultured

KW - Up-Regulation

KW - Vascular Endothelial Growth Factor A

KW - Vascular Endothelial Growth Factors

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1038/sj/neo/7900133

DO - 10.1038/sj/neo/7900133

M3 - Journal article

C2 - 11326319

SN - 1522-8002

VL - 3

SP - 80

EP - 87

JO - Neoplasia (New York, N.Y.)

JF - Neoplasia (New York, N.Y.)

IS - 1

ER -

Coregulation of glucose uptake and vascular endothelial growth factor (VEGF) in two small-cell lung cancer (SCLC) sublines in vivo and in vitro

Abstract

Keywords

UN SDGs

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