COP1 E3 ligase protects HYL1 to retain microRNA biogenesis

Seok Keun Cho; Samir Ben Chaabane; Pratik Shah; Christian Peter Poulsen; Seong Wook Yang

doi:10.1038/ncomms6867

COP1 E3 ligase protects HYL1 to retain microRNA biogenesis

Seok Keun Cho, Samir Ben Chaabane, Pratik Shah, Christian Peter Poulsen, Seong Wook Yang

Section for Plant Biochemistry

58 Citations (Scopus)

Abstract

Constitutive photomorphogenic 1 (COP1) is a RING-finger E3 ligase that plays a central role in photomorphogenesis by destabilizing many light-regulated transcription factors and photoreceptors. Here, we reveal a novel function for COP1 E3 ligase in controlling global miRNA biogenesis in Arabidopsis thaliana. In cop1 mutants, the level of miRNAs is dramatically reduced because of the diminution of HYPONASTIC LEAVES 1(HYL1), an RNA-binding protein required for precise miRNA processing. HYL1 is destabilized by an unidentified protease, which we tentatively call protease X, that specifically cleaves the N-terminal region from HYL1, thus neutralizing its function. Our results further show that the cytoplasmic partitioning of COP1 under light is essential to protect HYL1 against protease X. Taken together, we suggest a novel regulatory network involving HYL1, protease X, COP1 and light signalling that is indispensable for miRNA biogenesis in Arabidopsis thaliana.

Original language	English
Article number	5867
Journal	Nature Communications
Volume	5
Number of pages	10
ISSN	2041-1723
DOIs	https://doi.org/10.1038/ncomms6867
Publication status	Published - 2014

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title = "COP1 E3 ligase protects HYL1 to retain microRNA biogenesis",

abstract = "Constitutive photomorphogenic 1 (COP1) is a RING-finger E3 ligase that plays a central role in photomorphogenesis by destabilizing many light-regulated transcription factors and photoreceptors. Here, we reveal a novel function for COP1 E3 ligase in controlling global miRNA biogenesis in Arabidopsis thaliana. In cop1 mutants, the level of miRNAs is dramatically reduced because of the diminution of HYPONASTIC LEAVES 1(HYL1), an RNA-binding protein required for precise miRNA processing. HYL1 is destabilized by an unidentified protease, which we tentatively call protease X, that specifically cleaves the N-terminal region from HYL1, thus neutralizing its function. Our results further show that the cytoplasmic partitioning of COP1 under light is essential to protect HYL1 against protease X. Taken together, we suggest a novel regulatory network involving HYL1, protease X, COP1 and light signalling that is indispensable for miRNA biogenesis in Arabidopsis thaliana.",

author = "Cho, {Seok Keun} and {Ben Chaabane}, Samir and Pratik Shah and Poulsen, {Christian Peter} and Yang, {Seong Wook}",

year = "2014",

doi = "10.1038/ncomms6867",

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journal = "Nature Communications",

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T1 - COP1 E3 ligase protects HYL1 to retain microRNA biogenesis

AU - Cho, Seok Keun

AU - Ben Chaabane, Samir

AU - Shah, Pratik

AU - Poulsen, Christian Peter

AU - Yang, Seong Wook

PY - 2014

Y1 - 2014

N2 - Constitutive photomorphogenic 1 (COP1) is a RING-finger E3 ligase that plays a central role in photomorphogenesis by destabilizing many light-regulated transcription factors and photoreceptors. Here, we reveal a novel function for COP1 E3 ligase in controlling global miRNA biogenesis in Arabidopsis thaliana. In cop1 mutants, the level of miRNAs is dramatically reduced because of the diminution of HYPONASTIC LEAVES 1(HYL1), an RNA-binding protein required for precise miRNA processing. HYL1 is destabilized by an unidentified protease, which we tentatively call protease X, that specifically cleaves the N-terminal region from HYL1, thus neutralizing its function. Our results further show that the cytoplasmic partitioning of COP1 under light is essential to protect HYL1 against protease X. Taken together, we suggest a novel regulatory network involving HYL1, protease X, COP1 and light signalling that is indispensable for miRNA biogenesis in Arabidopsis thaliana.

AB - Constitutive photomorphogenic 1 (COP1) is a RING-finger E3 ligase that plays a central role in photomorphogenesis by destabilizing many light-regulated transcription factors and photoreceptors. Here, we reveal a novel function for COP1 E3 ligase in controlling global miRNA biogenesis in Arabidopsis thaliana. In cop1 mutants, the level of miRNAs is dramatically reduced because of the diminution of HYPONASTIC LEAVES 1(HYL1), an RNA-binding protein required for precise miRNA processing. HYL1 is destabilized by an unidentified protease, which we tentatively call protease X, that specifically cleaves the N-terminal region from HYL1, thus neutralizing its function. Our results further show that the cytoplasmic partitioning of COP1 under light is essential to protect HYL1 against protease X. Taken together, we suggest a novel regulatory network involving HYL1, protease X, COP1 and light signalling that is indispensable for miRNA biogenesis in Arabidopsis thaliana.

U2 - 10.1038/ncomms6867

DO - 10.1038/ncomms6867

M3 - Journal article

C2 - 25532508

SN - 2041-1723

VL - 5

JO - Nature Communications

JF - Nature Communications

M1 - 5867

ER -

COP1 E3 ligase protects HYL1 to retain microRNA biogenesis

Abstract

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