@inbook{3b2fdd501df111deb43e000ea68e967b,
title = "Construction and Use of Flow Cytometry Optimized Plasmid-Sensor Strains: Genomes in Flux",
abstract = "Determining the stability of plasmids in bacterial populations is traditionally performed by isolating a large number of clones followed by screening for the presence of plasmids by replica transfer to plasmid-selective agar plates. This is often a laborious task, especially when the intrinsic stability of the plasmid is high. The method presented here relies on a phenotypic (green fluorescence protein) marker, which is switched on if the host bacteria loses the residing plasmid. The incorporation of flow cytometry for single-cell detection and discrimination between plasmid-free and plasmid-harboring cells in a bacterial population facilitates a very high throughput of cells and thus provides excellent sensitivity and statistics toward detecting even very low levels of plasmid instability.",
author = "Bahl, {Martin Iain} and Gunnar Oregaard and S{\o}rensen, {S{\o}ren Johannes} and Hansen, {Lars H.}",
note = "Keywords Plasmid stability - green fluorescence protein - flow cytometry - plasmid-sensor strain - lac repressor",
year = "2009",
doi = "10.1007/978-1-60327-853-9_15",
language = "English",
isbn = "978-1-60327-852-2",
volume = "532",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "257--268",
editor = "Gogarten, {Maria Boekels} and Gogarten, {Johann Peter} and Olendzenski, {Lorraine C.}",
booktitle = "Horizontal Gene Transfer",
address = "United States",
}