Constructing and screening a metagenomic library of a cold and alkaline extreme environment

TY - CHAP

T1 - Constructing and screening a metagenomic library of a cold and alkaline extreme environment

AU - Glaring, Mikkel Andreas

AU - Vester, Jan Kjølhede

AU - Stougaard, Peter

PY - 2017

Y1 - 2017

N2 - Natural cold or alkaline environments are common on Earth. A rare combination of these two extremes is found in the permanently cold (less than 6 °C) and alkaline (pH above 10) ikaite columns in the Ikka Fjord in Southern Greenland. Bioprospecting efforts have established the ikaite columns as a source of bacteria and enzymes adapted to these conditions. They have also highlighted the limitations of cultivation-based methods in this extreme environment and metagenomic approaches may provide access to novel extremophilic enzymes from the uncultured majority of bacteria. Here, we describe the construction and screening of a metagenomic library of the prokaryotic community inhabiting the ikaite columns.

AB - Natural cold or alkaline environments are common on Earth. A rare combination of these two extremes is found in the permanently cold (less than 6 °C) and alkaline (pH above 10) ikaite columns in the Ikka Fjord in Southern Greenland. Bioprospecting efforts have established the ikaite columns as a source of bacteria and enzymes adapted to these conditions. They have also highlighted the limitations of cultivation-based methods in this extreme environment and metagenomic approaches may provide access to novel extremophilic enzymes from the uncultured majority of bacteria. Here, we describe the construction and screening of a metagenomic library of the prokaryotic community inhabiting the ikaite columns.

KW - Journal Article

U2 - 10.1007/978-1-4939-6691-2_4

DO - 10.1007/978-1-4939-6691-2_4

M3 - Book chapter

C2 - 27900683

SN - 978-1-4939-6689-9

T3 - Methods in Molecular Biology

SP - 43

EP - 55

BT - Metagenomics

A2 - Streit, Wolfgang R.

A2 - Daniel, Rolf

PB - Springer

ER -