Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle

Vernon G Coffey; Henriette Pilegaard; Andrew P Garnham; Brendan J O'Brien; John A Hawley

doi:10.1152/japplphysiol.91221.2008

Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle

Vernon G Coffey, Henriette Pilegaard, Andrew P Garnham, Brendan J O'Brien, John A Hawley

Molecular Integrative Physiology

68 Citations (Scopus)

Abstract

We examined acute molecular responses in skeletal muscle to divergent exercise stimuli by combining consecutive bouts of resistance and endurance exercise. Eight men [22.9 +/- 6.3 yr, body mass of 73.2 +/- 4.5 kg, peak O(2) uptake (Vo(2peak)) of 54.0 +/- 5.7 ml.kg(-1) x min(-1)] were randomly assigned to complete trials consisting of either resistance exercise (8 x 5 leg extension, 80% 1 repetition maximum) followed by a bout of endurance exercise (30 min cycling, 70% Vo(2peak)) or vice versa. Muscle biopsies were obtained from the vastus lateralis at rest, 15 min after each exercise bout, and after 3 h of passive recovery to determine early signaling and mRNA responses. Phosphorylation of Akt and Akt1(Ser473) were elevated 15 min after resistance exercise compared with cycling, with the greatest increase observed when resistance exercise followed cycling ( approximately 55%; P < 0.01). TSC2-mTOR-S6 kinase phosphorylation 15 min after each bout of exercise was similar regardless of the exercise mode. The cumulative effect of combined exercise resulted in disparate mRNA responses. IGF-I mRNA content was reduced when cycling preceded resistance exercise (-42%), whereas muscle ring finger mRNA was elevated when cycling was undertaken after resistance exercise ( approximately 52%; P < 0.05). The hexokinase II mRNA level was higher after resistance cycling ( approximately 45%; P < 0.05) than after cycling-resistance exercise, whereas modest increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha mRNA did not reveal an order effect. We conclude that acute responses to diverse bouts of contractile activity are modified by the exercise order. Moreover, undertaking divergent exercise in close proximity influences the acute molecular profile and likely exacerbates acute "interference."

Original language	English
Journal	Journal of Applied Physiology
Volume	106
Issue number	4
Pages (from-to)	1187-97
Number of pages	10
ISSN	8750-7587
DOIs	https://doi.org/10.1152/japplphysiol.91221.2008
Publication status	Published - 2009

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10.1152/japplphysiol.91221.2008

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title = "Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle",

abstract = "We examined acute molecular responses in skeletal muscle to divergent exercise stimuli by combining consecutive bouts of resistance and endurance exercise. Eight men [22.9 +/- 6.3 yr, body mass of 73.2 +/- 4.5 kg, peak O(2) uptake (Vo(2peak)) of 54.0 +/- 5.7 ml.kg(-1) x min(-1)] were randomly assigned to complete trials consisting of either resistance exercise (8 x 5 leg extension, 80% 1 repetition maximum) followed by a bout of endurance exercise (30 min cycling, 70% Vo(2peak)) or vice versa. Muscle biopsies were obtained from the vastus lateralis at rest, 15 min after each exercise bout, and after 3 h of passive recovery to determine early signaling and mRNA responses. Phosphorylation of Akt and Akt1(Ser473) were elevated 15 min after resistance exercise compared with cycling, with the greatest increase observed when resistance exercise followed cycling ( approximately 55%; P < 0.01). TSC2-mTOR-S6 kinase phosphorylation 15 min after each bout of exercise was similar regardless of the exercise mode. The cumulative effect of combined exercise resulted in disparate mRNA responses. IGF-I mRNA content was reduced when cycling preceded resistance exercise (-42%), whereas muscle ring finger mRNA was elevated when cycling was undertaken after resistance exercise ( approximately 52%; P < 0.05). The hexokinase II mRNA level was higher after resistance cycling ( approximately 45%; P < 0.05) than after cycling-resistance exercise, whereas modest increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha mRNA did not reveal an order effect. We conclude that acute responses to diverse bouts of contractile activity are modified by the exercise order. Moreover, undertaking divergent exercise in close proximity influences the acute molecular profile and likely exacerbates acute {"}interference.{"}",

author = "Coffey, {Vernon G} and Henriette Pilegaard and Garnham, {Andrew P} and O'Brien, {Brendan J} and Hawley, {John A}",

note = "Keywords: Adult; Anaerobic Threshold; Bicycling; Blood Glucose; Blotting, Western; Cross-Over Studies; Diet; Exercise; Glycogen; Humans; Lactic Acid; Male; Muscle Contraction; Muscle Strength; Muscle, Skeletal; Physical Endurance; Physical Fitness; RNA; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; Weight Lifting; Young Adult",

year = "2009",

doi = "10.1152/japplphysiol.91221.2008",

language = "English",

volume = "106",

pages = "1187--97",

journal = "Journal of Applied Physiology",

issn = "8750-7587",

publisher = "American Physiological Society",

number = "4",

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TY - JOUR

T1 - Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle

AU - Coffey, Vernon G

AU - Pilegaard, Henriette

AU - Garnham, Andrew P

AU - O'Brien, Brendan J

AU - Hawley, John A

N1 - Keywords: Adult; Anaerobic Threshold; Bicycling; Blood Glucose; Blotting, Western; Cross-Over Studies; Diet; Exercise; Glycogen; Humans; Lactic Acid; Male; Muscle Contraction; Muscle Strength; Muscle, Skeletal; Physical Endurance; Physical Fitness; RNA; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; Weight Lifting; Young Adult

PY - 2009

Y1 - 2009

N2 - We examined acute molecular responses in skeletal muscle to divergent exercise stimuli by combining consecutive bouts of resistance and endurance exercise. Eight men [22.9 +/- 6.3 yr, body mass of 73.2 +/- 4.5 kg, peak O(2) uptake (Vo(2peak)) of 54.0 +/- 5.7 ml.kg(-1) x min(-1)] were randomly assigned to complete trials consisting of either resistance exercise (8 x 5 leg extension, 80% 1 repetition maximum) followed by a bout of endurance exercise (30 min cycling, 70% Vo(2peak)) or vice versa. Muscle biopsies were obtained from the vastus lateralis at rest, 15 min after each exercise bout, and after 3 h of passive recovery to determine early signaling and mRNA responses. Phosphorylation of Akt and Akt1(Ser473) were elevated 15 min after resistance exercise compared with cycling, with the greatest increase observed when resistance exercise followed cycling ( approximately 55%; P < 0.01). TSC2-mTOR-S6 kinase phosphorylation 15 min after each bout of exercise was similar regardless of the exercise mode. The cumulative effect of combined exercise resulted in disparate mRNA responses. IGF-I mRNA content was reduced when cycling preceded resistance exercise (-42%), whereas muscle ring finger mRNA was elevated when cycling was undertaken after resistance exercise ( approximately 52%; P < 0.05). The hexokinase II mRNA level was higher after resistance cycling ( approximately 45%; P < 0.05) than after cycling-resistance exercise, whereas modest increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha mRNA did not reveal an order effect. We conclude that acute responses to diverse bouts of contractile activity are modified by the exercise order. Moreover, undertaking divergent exercise in close proximity influences the acute molecular profile and likely exacerbates acute "interference."

AB - We examined acute molecular responses in skeletal muscle to divergent exercise stimuli by combining consecutive bouts of resistance and endurance exercise. Eight men [22.9 +/- 6.3 yr, body mass of 73.2 +/- 4.5 kg, peak O(2) uptake (Vo(2peak)) of 54.0 +/- 5.7 ml.kg(-1) x min(-1)] were randomly assigned to complete trials consisting of either resistance exercise (8 x 5 leg extension, 80% 1 repetition maximum) followed by a bout of endurance exercise (30 min cycling, 70% Vo(2peak)) or vice versa. Muscle biopsies were obtained from the vastus lateralis at rest, 15 min after each exercise bout, and after 3 h of passive recovery to determine early signaling and mRNA responses. Phosphorylation of Akt and Akt1(Ser473) were elevated 15 min after resistance exercise compared with cycling, with the greatest increase observed when resistance exercise followed cycling ( approximately 55%; P < 0.01). TSC2-mTOR-S6 kinase phosphorylation 15 min after each bout of exercise was similar regardless of the exercise mode. The cumulative effect of combined exercise resulted in disparate mRNA responses. IGF-I mRNA content was reduced when cycling preceded resistance exercise (-42%), whereas muscle ring finger mRNA was elevated when cycling was undertaken after resistance exercise ( approximately 52%; P < 0.05). The hexokinase II mRNA level was higher after resistance cycling ( approximately 45%; P < 0.05) than after cycling-resistance exercise, whereas modest increases in peroxisome proliferator-activated receptor gamma coactivator-1alpha mRNA did not reveal an order effect. We conclude that acute responses to diverse bouts of contractile activity are modified by the exercise order. Moreover, undertaking divergent exercise in close proximity influences the acute molecular profile and likely exacerbates acute "interference."

U2 - 10.1152/japplphysiol.91221.2008

DO - 10.1152/japplphysiol.91221.2008

M3 - Journal article

C2 - 19164772

SN - 8750-7587

VL - 106

SP - 1187

EP - 1197

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

IS - 4

ER -

Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle

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