Comprehensive analysis of microrna expression in regionalized human neural progenitor cells reveals microRNA-10 as a caudalizing factor

TY - JOUR

T1 - Comprehensive analysis of microrna expression in regionalized human neural progenitor cells reveals microRNA-10 as a caudalizing factor

AU - Jönsson, Marie E.

AU - Wahlestedt, Jenny Nelander

AU - Åkerblom, Malin

AU - Kirkeby, Agnete

AU - Malmevik, Josephine

AU - Brattaas, Per Ludvik

AU - Jakobsson, Johan

AU - Parmar, Malin

PY - 2015/9/23

Y1 - 2015/9/23

N2 - MicroRNAs (miRNAs) have been implicated in regulating multiple processes during brain development in various species. However, the function of miRNAs in human brain development remains largely unexplored. Here, we provide a comprehensive analysis of miRNA expression of regionalized neural progenitor cells derived from human embryonic stem cells and human foetal brain. We found miR-92b-3p and miR-130b-5p to be specifically associated with neural progenitors and several miRNAs that display both age-specific and region-specific expression patterns. Among these miRNAs, we identified miR-10 to be specifically expressed in the human hindbrain and spinal cord, while being absent from rostral regions. We found that miR-10 regulates a large number of genes enriched for functions including transcription, actin cytoskeleton and ephrin receptor signalling. When overexpressed, miR-10 influences caudalization of human neural progenitor cells. Together, these data confirm a role for miRNAs in establishing different human neural progenitor populations. This dataset also provides a comprehensive resource for future studies investigating the functional role of different miRNAs in human brain development.

AB - MicroRNAs (miRNAs) have been implicated in regulating multiple processes during brain development in various species. However, the function of miRNAs in human brain development remains largely unexplored. Here, we provide a comprehensive analysis of miRNA expression of regionalized neural progenitor cells derived from human embryonic stem cells and human foetal brain. We found miR-92b-3p and miR-130b-5p to be specifically associated with neural progenitors and several miRNAs that display both age-specific and region-specific expression patterns. Among these miRNAs, we identified miR-10 to be specifically expressed in the human hindbrain and spinal cord, while being absent from rostral regions. We found that miR-10 regulates a large number of genes enriched for functions including transcription, actin cytoskeleton and ephrin receptor signalling. When overexpressed, miR-10 influences caudalization of human neural progenitor cells. Together, these data confirm a role for miRNAs in establishing different human neural progenitor populations. This dataset also provides a comprehensive resource for future studies investigating the functional role of different miRNAs in human brain development.

KW - Cell specification

KW - MicroRNA

KW - Neural development

KW - Neural stem cells

UR - http://www.scopus.com/inward/record.url?scp=84942163494&partnerID=8YFLogxK

U2 - 10.1242/dev.122747

DO - 10.1242/dev.122747

M3 - Journal article

C2 - 26395143

AN - SCOPUS:84942163494

SN - 0950-1991

VL - 142

SP - 3166

EP - 3177

JO - Development

JF - Development

IS - 18

ER -