Composition and structure of photosystem I in the moss Physcomitrella patens

Andreas Busch; Jørgen Petersen; Miriam T. Webber-Birungi; Marta Powikrowska; Lærke Marie Münter Lassen; Bianca Naumann-Busch; Agnieszka Janina Zygadlo Nielsen; Juanying Ye; Egbert J. Boekema; Ole Nørregaard Jensen; Christina Lunde; Poul Erik Jensen

doi:10.1093/jxb/ert126

Composition and structure of photosystem I in the moss Physcomitrella patens

Andreas Busch, Jørgen Petersen, Miriam T. Webber-Birungi, Marta Powikrowska, Lærke Marie Münter Lassen, Bianca Naumann-Busch, Agnieszka Janina Zygadlo Nielsen, Juanying Ye, Egbert J. Boekema, Ole Nørregaard Jensen, Christina Lunde, Poul Erik Jensen

25 Citations (Scopus)

Abstract

Recently, bryophytes, which diverged from the ancestor of seed plants more than 400 million years ago, came into focus in photosynthesis research as they can provide valuable insights into the evolution of photosynthetic complexes during the adaptation to terrestrial life. This study isolated intact photosystem I (PSI) with its associated light-harvesting complex (LHCI) from the moss Physcomitrella patens and characterized its structure, polypeptide composition, and light-harvesting function using electron microscopy, mass spectrometry, biochemical, and physiological methods. It became evident that Physcomitrella possesses a strikingly high number of isoforms for the different PSI core subunits as well as LHCI proteins. It was demonstrated that all these different subunit isoforms are expressed at the protein level and are incorporated into functional PSI-LHCI complexes. Furthermore, in contrast to previous reports, it was demonstrated that Physcomitrella assembles a light-harvesting complex consisting of four light-harvesting proteins forming a higher-plant-like PSI superstructure.

Original language	English
Journal	Journal of Experimental Botany
Volume	64
Issue number	10
Pages (from-to)	2689-2699
Number of pages	11
ISSN	0022-0957
DOIs	https://doi.org/10.1093/jxb/ert126
Publication status	Published - Jul 2013

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@article{a181b228162641579fc19a69b7c349bd,

title = "Composition and structure of photosystem I in the moss Physcomitrella patens",

abstract = "Recently, bryophytes, which diverged from the ancestor of seed plants more than 400 million years ago, came into focus in photosynthesis research as they can provide valuable insights into the evolution of photosynthetic complexes during the adaptation to terrestrial life. This study isolated intact photosystem I (PSI) with its associated light-harvesting complex (LHCI) from the moss Physcomitrella patens and characterized its structure, polypeptide composition, and light-harvesting function using electron microscopy, mass spectrometry, biochemical, and physiological methods. It became evident that Physcomitrella possesses a strikingly high number of isoforms for the different PSI core subunits as well as LHCI proteins. It was demonstrated that all these different subunit isoforms are expressed at the protein level and are incorporated into functional PSI-LHCI complexes. Furthermore, in contrast to previous reports, it was demonstrated that Physcomitrella assembles a light-harvesting complex consisting of four light-harvesting proteins forming a higher-plant-like PSI superstructure.",

author = "Andreas Busch and J{\o}rgen Petersen and Webber-Birungi, {Miriam T.} and Marta Powikrowska and Lassen, {L{\ae}rke Marie M{\"u}nter} and Bianca Naumann-Busch and Nielsen, {Agnieszka Janina Zygadlo} and Juanying Ye and Boekema, {Egbert J.} and Jensen, {Ole N{\o}rregaard} and Christina Lunde and Jensen, {Poul Erik}",

year = "2013",

month = jul,

doi = "10.1093/jxb/ert126",

language = "English",

volume = "64",

pages = "2689--2699",

journal = "Journal of Experimental Botany",

issn = "0022-0957",

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TY - JOUR

T1 - Composition and structure of photosystem I in the moss Physcomitrella patens

AU - Busch, Andreas

AU - Petersen, Jørgen

AU - Webber-Birungi, Miriam T.

AU - Powikrowska, Marta

AU - Lassen, Lærke Marie Münter

AU - Naumann-Busch, Bianca

AU - Nielsen, Agnieszka Janina Zygadlo

AU - Ye, Juanying

AU - Boekema, Egbert J.

AU - Jensen, Ole Nørregaard

AU - Lunde, Christina

AU - Jensen, Poul Erik

PY - 2013/7

Y1 - 2013/7

N2 - Recently, bryophytes, which diverged from the ancestor of seed plants more than 400 million years ago, came into focus in photosynthesis research as they can provide valuable insights into the evolution of photosynthetic complexes during the adaptation to terrestrial life. This study isolated intact photosystem I (PSI) with its associated light-harvesting complex (LHCI) from the moss Physcomitrella patens and characterized its structure, polypeptide composition, and light-harvesting function using electron microscopy, mass spectrometry, biochemical, and physiological methods. It became evident that Physcomitrella possesses a strikingly high number of isoforms for the different PSI core subunits as well as LHCI proteins. It was demonstrated that all these different subunit isoforms are expressed at the protein level and are incorporated into functional PSI-LHCI complexes. Furthermore, in contrast to previous reports, it was demonstrated that Physcomitrella assembles a light-harvesting complex consisting of four light-harvesting proteins forming a higher-plant-like PSI superstructure.

AB - Recently, bryophytes, which diverged from the ancestor of seed plants more than 400 million years ago, came into focus in photosynthesis research as they can provide valuable insights into the evolution of photosynthetic complexes during the adaptation to terrestrial life. This study isolated intact photosystem I (PSI) with its associated light-harvesting complex (LHCI) from the moss Physcomitrella patens and characterized its structure, polypeptide composition, and light-harvesting function using electron microscopy, mass spectrometry, biochemical, and physiological methods. It became evident that Physcomitrella possesses a strikingly high number of isoforms for the different PSI core subunits as well as LHCI proteins. It was demonstrated that all these different subunit isoforms are expressed at the protein level and are incorporated into functional PSI-LHCI complexes. Furthermore, in contrast to previous reports, it was demonstrated that Physcomitrella assembles a light-harvesting complex consisting of four light-harvesting proteins forming a higher-plant-like PSI superstructure.

U2 - 10.1093/jxb/ert126

DO - 10.1093/jxb/ert126

M3 - Journal article

C2 - 23682117

SN - 0022-0957

VL - 64

SP - 2689

EP - 2699

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

IS - 10

ER -

Composition and structure of photosystem I in the moss Physcomitrella patens

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