Complex plastic changes in the neuropeptide Y system during ethanol intoxication and withdrawal in the rat brain

J D Olling; J Ulrichsen; D Z Christensen; D P D Woldbye; J D Olling; J Ulrichsen; D Z Christensen; D P D Woldbye

doi:10.1002/jnr.22049

Complex plastic changes in the neuropeptide Y system during ethanol intoxication and withdrawal in the rat brain

J D Olling, J Ulrichsen, D Z Christensen, D P D Woldbye, J D Olling, J Ulrichsen, D Z Christensen, D P D Woldbye

9 Citations (Scopus)

Abstract

Previous studies show that chronic ethanol treatment induces prominent changes in brain neuropeptide Y (NPY). The purpose of the present study was to explore ethanol effects at a deeper NPY-system level, measuring expression of NPY and its receptors (Y1, Y2, Y5) as well as NPY receptor binding and NPY-stimulated [(35)S]GTPgammaS functional binding. Rats received intragastric ethanol repeatedly for 4 days, and the NPY system was studied in the hippocampal dentate gyrus (DG), CA3, CA1, and piriform cortex (PirCx) and neocortex (NeoCx) during intoxication, peak withdrawal (16 hr), late withdrawal (3 days), and 1 week after last ethanol administration. NPY mRNA levels decreased during intoxication and at 16 hr in hippocampal regions but increased in the PirCx and NeoCx at 16 hr. NPY mRNA levels were increased at 3 days and returned to control levels in most regions at 1 week. Substantial changes also occurred at the receptor level. Thus Y1, Y2, and Y5 mRNA labelling decreased at 16 hr in most regions, returning to control levels at 3 days, except for PirCx Y2 mRNA, which increased at 3 days and 1 week. Conversely, increases in NPY receptor binding occurred in hippocampal regions during intoxication and in functional binding in the DG and NeoCx during intoxication and at 16 hr and in PirCx during intoxication and at 1 week. Thus this study shows that ethanol intoxication and withdrawal induce complex plastic changes in the NPY system, with decreased/increased gene expression or binding occurring in a time- and region-specific manner. These changes may play an important role in mediating ethanol-induced changes in neuronal excitability.

Original language	English
Journal	Journal of Neuroscience Research
Volume	87
Issue number	10
Pages (from-to)	2386-97
Number of pages	11
ISSN	0360-4012
DOIs	https://doi.org/10.1002/jnr.22049 https://doi.org/10.1002/jnr.22049
Publication status	Published - 2009

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Olling, J. D., Ulrichsen, J., Christensen, D. Z., Woldbye, D. P. D., Olling, J. D., Ulrichsen, J., Christensen, D. Z., & Woldbye, D. P. D. (2009). Complex plastic changes in the neuropeptide Y system during ethanol intoxication and withdrawal in the rat brain. Journal of Neuroscience Research, 87(10), 2386-97. https://doi.org/10.1002/jnr.22049, https://doi.org/10.1002/jnr.22049

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title = "Complex plastic changes in the neuropeptide Y system during ethanol intoxication and withdrawal in the rat brain",

abstract = "Previous studies show that chronic ethanol treatment induces prominent changes in brain neuropeptide Y (NPY). The purpose of the present study was to explore ethanol effects at a deeper NPY-system level, measuring expression of NPY and its receptors (Y1, Y2, Y5) as well as NPY receptor binding and NPY-stimulated [(35)S]GTPgammaS functional binding. Rats received intragastric ethanol repeatedly for 4 days, and the NPY system was studied in the hippocampal dentate gyrus (DG), CA3, CA1, and piriform cortex (PirCx) and neocortex (NeoCx) during intoxication, peak withdrawal (16 hr), late withdrawal (3 days), and 1 week after last ethanol administration. NPY mRNA levels decreased during intoxication and at 16 hr in hippocampal regions but increased in the PirCx and NeoCx at 16 hr. NPY mRNA levels were increased at 3 days and returned to control levels in most regions at 1 week. Substantial changes also occurred at the receptor level. Thus Y1, Y2, and Y5 mRNA labelling decreased at 16 hr in most regions, returning to control levels at 3 days, except for PirCx Y2 mRNA, which increased at 3 days and 1 week. Conversely, increases in NPY receptor binding occurred in hippocampal regions during intoxication and in functional binding in the DG and NeoCx during intoxication and at 16 hr and in PirCx during intoxication and at 1 week. Thus this study shows that ethanol intoxication and withdrawal induce complex plastic changes in the NPY system, with decreased/increased gene expression or binding occurring in a time- and region-specific manner. These changes may play an important role in mediating ethanol-induced changes in neuronal excitability.",

author = "Olling, {J D} and J Ulrichsen and Christensen, {D Z} and Woldbye, {D P D} and Olling, {J D} and J Ulrichsen and Christensen, {D Z} and Woldbye, {D P D}",

note = "Keywords: Alcohol-Induced Disorders, Nervous System; Analysis of Variance; Animals; Brain; Disease Models, Animal; Guanosine 5'-O-(3-Thiotriphosphate); Male; Neuronal Plasticity; Neuropeptide Y; Protein Binding; RNA, Messenger; Rats; Rats, Wistar; Receptors, Neuropeptide Y; Substance Withdrawal Syndrome; Sulfur Isotopes; Time Factors; Up-Regulation",

year = "2009",

doi = "10.1002/jnr.22049",

language = "English",

volume = "87",

pages = "2386--97",

journal = "Journal of Neuroscience Research",

issn = "0360-4012",

publisher = "JohnWiley & Sons, Inc.",

number = "10",

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TY - JOUR

T1 - Complex plastic changes in the neuropeptide Y system during ethanol intoxication and withdrawal in the rat brain

AU - Olling, J D

AU - Ulrichsen, J

AU - Christensen, D Z

AU - Woldbye, D P D

AU - Olling, J D

AU - Ulrichsen, J

AU - Christensen, D Z

AU - Woldbye, D P D

N1 - Keywords: Alcohol-Induced Disorders, Nervous System; Analysis of Variance; Animals; Brain; Disease Models, Animal; Guanosine 5'-O-(3-Thiotriphosphate); Male; Neuronal Plasticity; Neuropeptide Y; Protein Binding; RNA, Messenger; Rats; Rats, Wistar; Receptors, Neuropeptide Y; Substance Withdrawal Syndrome; Sulfur Isotopes; Time Factors; Up-Regulation

PY - 2009

Y1 - 2009

N2 - Previous studies show that chronic ethanol treatment induces prominent changes in brain neuropeptide Y (NPY). The purpose of the present study was to explore ethanol effects at a deeper NPY-system level, measuring expression of NPY and its receptors (Y1, Y2, Y5) as well as NPY receptor binding and NPY-stimulated [(35)S]GTPgammaS functional binding. Rats received intragastric ethanol repeatedly for 4 days, and the NPY system was studied in the hippocampal dentate gyrus (DG), CA3, CA1, and piriform cortex (PirCx) and neocortex (NeoCx) during intoxication, peak withdrawal (16 hr), late withdrawal (3 days), and 1 week after last ethanol administration. NPY mRNA levels decreased during intoxication and at 16 hr in hippocampal regions but increased in the PirCx and NeoCx at 16 hr. NPY mRNA levels were increased at 3 days and returned to control levels in most regions at 1 week. Substantial changes also occurred at the receptor level. Thus Y1, Y2, and Y5 mRNA labelling decreased at 16 hr in most regions, returning to control levels at 3 days, except for PirCx Y2 mRNA, which increased at 3 days and 1 week. Conversely, increases in NPY receptor binding occurred in hippocampal regions during intoxication and in functional binding in the DG and NeoCx during intoxication and at 16 hr and in PirCx during intoxication and at 1 week. Thus this study shows that ethanol intoxication and withdrawal induce complex plastic changes in the NPY system, with decreased/increased gene expression or binding occurring in a time- and region-specific manner. These changes may play an important role in mediating ethanol-induced changes in neuronal excitability.

AB - Previous studies show that chronic ethanol treatment induces prominent changes in brain neuropeptide Y (NPY). The purpose of the present study was to explore ethanol effects at a deeper NPY-system level, measuring expression of NPY and its receptors (Y1, Y2, Y5) as well as NPY receptor binding and NPY-stimulated [(35)S]GTPgammaS functional binding. Rats received intragastric ethanol repeatedly for 4 days, and the NPY system was studied in the hippocampal dentate gyrus (DG), CA3, CA1, and piriform cortex (PirCx) and neocortex (NeoCx) during intoxication, peak withdrawal (16 hr), late withdrawal (3 days), and 1 week after last ethanol administration. NPY mRNA levels decreased during intoxication and at 16 hr in hippocampal regions but increased in the PirCx and NeoCx at 16 hr. NPY mRNA levels were increased at 3 days and returned to control levels in most regions at 1 week. Substantial changes also occurred at the receptor level. Thus Y1, Y2, and Y5 mRNA labelling decreased at 16 hr in most regions, returning to control levels at 3 days, except for PirCx Y2 mRNA, which increased at 3 days and 1 week. Conversely, increases in NPY receptor binding occurred in hippocampal regions during intoxication and in functional binding in the DG and NeoCx during intoxication and at 16 hr and in PirCx during intoxication and at 1 week. Thus this study shows that ethanol intoxication and withdrawal induce complex plastic changes in the NPY system, with decreased/increased gene expression or binding occurring in a time- and region-specific manner. These changes may play an important role in mediating ethanol-induced changes in neuronal excitability.

U2 - 10.1002/jnr.22049

DO - 10.1002/jnr.22049

M3 - Journal article

C2 - 19267419

SN - 0360-4012

VL - 87

SP - 2386

EP - 2397

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

IS - 10

ER -

Complex plastic changes in the neuropeptide Y system during ethanol intoxication and withdrawal in the rat brain

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