Complete genome sequence of the cystic fibrosis pathogen Achromobacter xylosoxidans NH44784-1996 complies with important pathogenic phenotypes

Tim Holm Jakobsen; Martin Asser Hansen; Peter Østrup Jensen; Lars Hansen; Leise Riber; April Patricia Indera Cockburn; Mette Kolpen; Christine Rønne Hansen; Winnie Ridderberg; Steffen Robert Eickhardt-Sørensen; Marlene Hansen; Peter Kerpedjiev; Morten Alhede; Klaus Qvortrup; Mette Burmølle; Claus Ernst Moser; Michael Kühl; Oana Ciofu; Michael Givskov; Søren Johannes Sørensen; Niels Høiby; Thomas Bjarnsholt

doi:10.1371/journal.pone.0068484

Complete genome sequence of the cystic fibrosis pathogen Achromobacter xylosoxidans NH44784-1996 complies with important pathogenic phenotypes

Tim Holm Jakobsen, Martin Asser Hansen, Peter Østrup Jensen, Lars Hansen, Leise Riber, April Patricia Indera Cockburn, Mette Kolpen, Christine Rønne Hansen, Winnie Ridderberg, Steffen Robert Eickhardt-Sørensen, Marlene Hansen, Peter Kerpedjiev, Morten Alhede, Klaus Qvortrup, Mette Burmølle, Claus Ernst Moser, Michael Kühl, Oana Ciofu, Michael Givskov, Søren Johannes SørensenNiels Høiby, Thomas Bjarnsholt

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Abstract

Achromobacter xylosoxidans is an environmental opportunistic pathogen, which infects an increasing number of immunocompromised patients. In this study we combined genomic analysis of a clinical isolated A. xylosoxidans. strain with phenotypic investigations of its important pathogenic features. We present a complete assembly of the genome of A. xylosoxidans NH44784-1996, an isolate from a cystic fibrosis patient obtained in 1996. The genome of in 1996. The genom NH44784-1996 contains approximately 7 million base pairs with 6390 potential protein-coding sequences. We identified several features that render it an opportunistic human pathogen, We found genes involved in anaerobic growth and the pgaABCD operon encoding the biofilm adhesin poly-β-1,6-N-acetyl-D-glucosamin. Furthermore, the genome contains a range of antibiotic resistance genes coding efflux pump systems and antibiotic modifying enzymes. In vitro studies of in 1996. The genom NH44784-1996 confirmed the genomic evidence for its ability to form biofilms, anaerobic growth via denitrification, and resistance to a broad range of antibiotics. Our investigation enables further studies of the functionality of important identified genes contributing to the pathogenicity of in 1996. The genom and thereby improves our understanding and ability to treat this emerging pathogen.

Original language	English
Article number	e68484
Journal	P L o S One
Volume	8
Issue number	7
Number of pages	17
ISSN	1932-6203
DOIs	https://doi.org/10.1371/journal.pone.0068484
Publication status	Published - 22 Jul 2013

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Jakobsen, T. H., Hansen, M. A., Jensen, P. Ø., Hansen, L., Riber, L., Cockburn, A. P. I., Kolpen, M., Hansen, C. R., Ridderberg, W., Eickhardt-Sørensen, S. R., Hansen, M., Kerpedjiev, P., Alhede, M., Qvortrup, K., Burmølle, M., Moser, C. E., Kühl, M., Ciofu, O., Givskov, M., ... Bjarnsholt, T. (2013). Complete genome sequence of the cystic fibrosis pathogen Achromobacter xylosoxidans NH44784-1996 complies with important pathogenic phenotypes. P L o S One, 8(7), Article e68484. https://doi.org/10.1371/journal.pone.0068484

Jakobsen, TH, Hansen, MA, Jensen, PØ, Hansen, L, Riber, L, Cockburn, API, Kolpen, M, Hansen, CR, Ridderberg, W, Eickhardt-Sørensen, SR, Hansen, M, Kerpedjiev, P, Alhede, M, Qvortrup, K , Burmølle, M , Moser, CE , Kühl, M , Ciofu, O , Givskov, M , Sørensen, SJ , Høiby, N & Bjarnsholt, T 2013, 'Complete genome sequence of the cystic fibrosis pathogen Achromobacter xylosoxidans NH44784-1996 complies with important pathogenic phenotypes', P L o S One, vol. 8, no. 7, e68484. https://doi.org/10.1371/journal.pone.0068484

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title = "Complete genome sequence of the cystic fibrosis pathogen Achromobacter xylosoxidans NH44784-1996 complies with important pathogenic phenotypes",

abstract = "Achromobacter xylosoxidans is an environmental opportunistic pathogen, which infects an increasing number of immunocompromised patients. In this study we combined genomic analysis of a clinical isolated A. xylosoxidans. strain with phenotypic investigations of its important pathogenic features. We present a complete assembly of the genome of A. xylosoxidans NH44784-1996, an isolate from a cystic fibrosis patient obtained in 1996. The genome of in 1996. The genom NH44784-1996 contains approximately 7 million base pairs with 6390 potential protein-coding sequences. We identified several features that render it an opportunistic human pathogen, We found genes involved in anaerobic growth and the pgaABCD operon encoding the biofilm adhesin poly-β-1,6-N-acetyl-D-glucosamin. Furthermore, the genome contains a range of antibiotic resistance genes coding efflux pump systems and antibiotic modifying enzymes. In vitro studies of in 1996. The genom NH44784-1996 confirmed the genomic evidence for its ability to form biofilms, anaerobic growth via denitrification, and resistance to a broad range of antibiotics. Our investigation enables further studies of the functionality of important identified genes contributing to the pathogenicity of in 1996. The genom and thereby improves our understanding and ability to treat this emerging pathogen.",

author = "Jakobsen, {Tim Holm} and Hansen, {Martin Asser} and Jensen, {Peter {\O}strup} and Lars Hansen and Leise Riber and Cockburn, {April Patricia Indera} and Mette Kolpen and Hansen, {Christine R{\o}nne} and Winnie Ridderberg and Eickhardt-S{\o}rensen, {Steffen Robert} and Marlene Hansen and Peter Kerpedjiev and Morten Alhede and Klaus Qvortrup and Mette Burm{\o}lle and Moser, {Claus Ernst} and Michael K{\"u}hl and Oana Ciofu and Michael Givskov and S{\o}rensen, {S{\o}ren Johannes} and Niels H{\o}iby and Thomas Bjarnsholt",

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T1 - Complete genome sequence of the cystic fibrosis pathogen Achromobacter xylosoxidans NH44784-1996 complies with important pathogenic phenotypes

AU - Jakobsen, Tim Holm

AU - Hansen, Martin Asser

AU - Jensen, Peter Østrup

AU - Hansen, Lars

AU - Riber, Leise

AU - Cockburn, April Patricia Indera

AU - Kolpen, Mette

AU - Hansen, Christine Rønne

AU - Ridderberg, Winnie

AU - Eickhardt-Sørensen, Steffen Robert

AU - Hansen, Marlene

AU - Kerpedjiev, Peter

AU - Alhede, Morten

AU - Qvortrup, Klaus

AU - Burmølle, Mette

AU - Moser, Claus Ernst

AU - Kühl, Michael

AU - Ciofu, Oana

AU - Givskov, Michael

AU - Sørensen, Søren Johannes

AU - Høiby, Niels

AU - Bjarnsholt, Thomas

PY - 2013/7/22

Y1 - 2013/7/22

N2 - Achromobacter xylosoxidans is an environmental opportunistic pathogen, which infects an increasing number of immunocompromised patients. In this study we combined genomic analysis of a clinical isolated A. xylosoxidans. strain with phenotypic investigations of its important pathogenic features. We present a complete assembly of the genome of A. xylosoxidans NH44784-1996, an isolate from a cystic fibrosis patient obtained in 1996. The genome of in 1996. The genom NH44784-1996 contains approximately 7 million base pairs with 6390 potential protein-coding sequences. We identified several features that render it an opportunistic human pathogen, We found genes involved in anaerobic growth and the pgaABCD operon encoding the biofilm adhesin poly-β-1,6-N-acetyl-D-glucosamin. Furthermore, the genome contains a range of antibiotic resistance genes coding efflux pump systems and antibiotic modifying enzymes. In vitro studies of in 1996. The genom NH44784-1996 confirmed the genomic evidence for its ability to form biofilms, anaerobic growth via denitrification, and resistance to a broad range of antibiotics. Our investigation enables further studies of the functionality of important identified genes contributing to the pathogenicity of in 1996. The genom and thereby improves our understanding and ability to treat this emerging pathogen.

AB - Achromobacter xylosoxidans is an environmental opportunistic pathogen, which infects an increasing number of immunocompromised patients. In this study we combined genomic analysis of a clinical isolated A. xylosoxidans. strain with phenotypic investigations of its important pathogenic features. We present a complete assembly of the genome of A. xylosoxidans NH44784-1996, an isolate from a cystic fibrosis patient obtained in 1996. The genome of in 1996. The genom NH44784-1996 contains approximately 7 million base pairs with 6390 potential protein-coding sequences. We identified several features that render it an opportunistic human pathogen, We found genes involved in anaerobic growth and the pgaABCD operon encoding the biofilm adhesin poly-β-1,6-N-acetyl-D-glucosamin. Furthermore, the genome contains a range of antibiotic resistance genes coding efflux pump systems and antibiotic modifying enzymes. In vitro studies of in 1996. The genom NH44784-1996 confirmed the genomic evidence for its ability to form biofilms, anaerobic growth via denitrification, and resistance to a broad range of antibiotics. Our investigation enables further studies of the functionality of important identified genes contributing to the pathogenicity of in 1996. The genom and thereby improves our understanding and ability to treat this emerging pathogen.

U2 - 10.1371/journal.pone.0068484

DO - 10.1371/journal.pone.0068484

M3 - Journal article

C2 - 23894309

SN - 1932-6203

VL - 8

JO - P L o S One

JF - P L o S One

IS - 7

M1 - e68484

ER -

Complete genome sequence of the cystic fibrosis pathogen Achromobacter xylosoxidans NH44784-1996 complies with important pathogenic phenotypes

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