Comparison of electron capture dissociation and collisionally activated dissociation of polycations of peptide nucleic acids

Jesper Velgaard Olsen; K F Haselmann; Michael Lund Nielsen; B A Budnik; Peter E. Nielsen; R A Zubarev

doi:10.1002/rcm.317

Comparison of electron capture dissociation and collisionally activated dissociation of polycations of peptide nucleic acids

Jesper Velgaard Olsen, K F Haselmann, Michael Lund Nielsen, B A Budnik, Peter E. Nielsen, R A Zubarev

31 Citations (Scopus)

Abstract

Electron capture dissociation (ECD) in Fourier transform ion cyclotron resonance mass spectrometry coupled with electrospray ionization enhances the sequence elucidation of peptide nucleic acids compared with conventional low-energy collisionally activated dissociation (CAD). Examples are shown where ECD produced complete or extensive sequence coverage in PNAs six to ten nucleobases long. However, facile base losses from the reduced species and low abundances of backbone ECD fragments presented a significant problem. This was rationalized through the lower degree of charge solvation on the backbone compared to polypeptides. Combination of both CAD and ECD data is advantageous, as these techniques produce cleavages at different sites.

Original language	English
Journal	Rapid Communications in Mass Spectrometry
Volume	15
Issue number	12
Pages (from-to)	969-74
Number of pages	5
ISSN	0951-4198
DOIs	https://doi.org/10.1002/rcm.317
Publication status	Published - 2001
Externally published	Yes

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title = "Comparison of electron capture dissociation and collisionally activated dissociation of polycations of peptide nucleic acids",

abstract = "Electron capture dissociation (ECD) in Fourier transform ion cyclotron resonance mass spectrometry coupled with electrospray ionization enhances the sequence elucidation of peptide nucleic acids compared with conventional low-energy collisionally activated dissociation (CAD). Examples are shown where ECD produced complete or extensive sequence coverage in PNAs six to ten nucleobases long. However, facile base losses from the reduced species and low abundances of backbone ECD fragments presented a significant problem. This was rationalized through the lower degree of charge solvation on the backbone compared to polypeptides. Combination of both CAD and ECD data is advantageous, as these techniques produce cleavages at different sites.",

author = "Olsen, {Jesper Velgaard} and Haselmann, {K F} and Nielsen, {Michael Lund} and Budnik, {B A} and Nielsen, {Peter E.} and Zubarev, {R A}",

note = "Keywords: Cations; Cyclotrons; Fourier Analysis; Nucleic Acids; Peptides; Spectrometry, Mass, Electrospray Ionization",

year = "2001",

doi = "10.1002/rcm.317",

language = "English",

volume = "15",

pages = "969--74",

journal = "Rapid Communications in Mass Spectrometry",

issn = "0951-4198",

publisher = "JohnWiley & Sons Ltd",

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TY - JOUR

T1 - Comparison of electron capture dissociation and collisionally activated dissociation of polycations of peptide nucleic acids

AU - Olsen, Jesper Velgaard

AU - Haselmann, K F

AU - Nielsen, Michael Lund

AU - Budnik, B A

AU - Nielsen, Peter E.

AU - Zubarev, R A

N1 - Keywords: Cations; Cyclotrons; Fourier Analysis; Nucleic Acids; Peptides; Spectrometry, Mass, Electrospray Ionization

PY - 2001

Y1 - 2001

N2 - Electron capture dissociation (ECD) in Fourier transform ion cyclotron resonance mass spectrometry coupled with electrospray ionization enhances the sequence elucidation of peptide nucleic acids compared with conventional low-energy collisionally activated dissociation (CAD). Examples are shown where ECD produced complete or extensive sequence coverage in PNAs six to ten nucleobases long. However, facile base losses from the reduced species and low abundances of backbone ECD fragments presented a significant problem. This was rationalized through the lower degree of charge solvation on the backbone compared to polypeptides. Combination of both CAD and ECD data is advantageous, as these techniques produce cleavages at different sites.

AB - Electron capture dissociation (ECD) in Fourier transform ion cyclotron resonance mass spectrometry coupled with electrospray ionization enhances the sequence elucidation of peptide nucleic acids compared with conventional low-energy collisionally activated dissociation (CAD). Examples are shown where ECD produced complete or extensive sequence coverage in PNAs six to ten nucleobases long. However, facile base losses from the reduced species and low abundances of backbone ECD fragments presented a significant problem. This was rationalized through the lower degree of charge solvation on the backbone compared to polypeptides. Combination of both CAD and ECD data is advantageous, as these techniques produce cleavages at different sites.

U2 - 10.1002/rcm.317

DO - 10.1002/rcm.317

M3 - Journal article

C2 - 11400205

SN - 0951-4198

VL - 15

SP - 969

EP - 974

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

IS - 12

ER -

Comparison of electron capture dissociation and collisionally activated dissociation of polycations of peptide nucleic acids

Abstract

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