Comparative study of PCR methods to detect Pasteurella multocida

Sadhana Adhikary; Magne Bisgaard; Geoffrey  Foster; Nathalie  Kiessling; Anna Rise Fahlen; John Elmerdahl Olsen; Henrik Christensen

doi:10.2376/0005-9366-126-415

Comparative study of PCR methods to detect Pasteurella multocida

Sadhana Adhikary, Magne Bisgaard, Geoffrey Foster, Nathalie Kiessling, Anna Rise Fahlen, John Elmerdahl Olsen, Henrik Christensen

4 Citations (Scopus)

Abstract

The aim of the investigation was to compare four published PCR methods for specific detection of Pasteurella multocida. 85 strains of P. multocida and 13 strains of other taxa were included in the comparison of four published PCR methods based on kmt1, 23S rRNA and transcriptional regulator genes pm0762 and pm1231 as targets, respectively. Sensitivities were calculated to 100 and 80% for the kmt1 and 23S rRNA based PCRs, respectively and specificities to 92 and 23%, respectively of the kmt1 and 23S rRNA based PCRs. The methods based on transcriptional regulator genes tested two Bisgaard taxon 45 strains positive in addition to one Bisgaard taxon 16 strain. Only the kmt1 method detected P. multocida with the expected sensitivity and specificity and this method is recommended for identification of the species.

Original language	English
Journal	Berliner und Muenchener Tieraerztliche Wochenschrift
Volume	126
Issue number	9/10
Pages (from-to)	415-422
Number of pages	8
ISSN	0005-9366
DOIs	https://doi.org/10.2376/0005-9366-126-415
Publication status	Published - 2013

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title = "Comparative study of PCR methods to detect Pasteurella multocida",

abstract = "The aim of the investigation was to compare four published PCR methods for specific detection of Pasteurella multocida. 85 strains of P. multocida and 13 strains of other taxa were included in the comparison of four published PCR methods based on kmt1, 23S rRNA and transcriptional regulator genes pm0762 and pm1231 as targets, respectively. Sensitivities were calculated to 100 and 80% for the kmt1 and 23S rRNA based PCRs, respectively and specificities to 92 and 23%, respectively of the kmt1 and 23S rRNA based PCRs. The methods based on transcriptional regulator genes tested two Bisgaard taxon 45 strains positive in addition to one Bisgaard taxon 16 strain. Only the kmt1 method detected P. multocida with the expected sensitivity and specificity and this method is recommended for identification of the species.",

author = "Sadhana Adhikary and Magne Bisgaard and Geoffrey Foster and Nathalie Kiessling and Fahlen, {Anna Rise} and Olsen, {John Elmerdahl} and Henrik Christensen",

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doi = "10.2376/0005-9366-126-415",

language = "English",

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pages = "415--422",

journal = "Berliner und Muenchener Tieraerztliche Wochenschrift",

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T1 - Comparative study of PCR methods to detect Pasteurella multocida

AU - Adhikary, Sadhana

AU - Bisgaard, Magne

AU - Foster, Geoffrey

AU - Kiessling, Nathalie

AU - Fahlen, Anna Rise

AU - Olsen, John Elmerdahl

AU - Christensen, Henrik

PY - 2013

Y1 - 2013

N2 - The aim of the investigation was to compare four published PCR methods for specific detection of Pasteurella multocida. 85 strains of P. multocida and 13 strains of other taxa were included in the comparison of four published PCR methods based on kmt1, 23S rRNA and transcriptional regulator genes pm0762 and pm1231 as targets, respectively. Sensitivities were calculated to 100 and 80% for the kmt1 and 23S rRNA based PCRs, respectively and specificities to 92 and 23%, respectively of the kmt1 and 23S rRNA based PCRs. The methods based on transcriptional regulator genes tested two Bisgaard taxon 45 strains positive in addition to one Bisgaard taxon 16 strain. Only the kmt1 method detected P. multocida with the expected sensitivity and specificity and this method is recommended for identification of the species.

AB - The aim of the investigation was to compare four published PCR methods for specific detection of Pasteurella multocida. 85 strains of P. multocida and 13 strains of other taxa were included in the comparison of four published PCR methods based on kmt1, 23S rRNA and transcriptional regulator genes pm0762 and pm1231 as targets, respectively. Sensitivities were calculated to 100 and 80% for the kmt1 and 23S rRNA based PCRs, respectively and specificities to 92 and 23%, respectively of the kmt1 and 23S rRNA based PCRs. The methods based on transcriptional regulator genes tested two Bisgaard taxon 45 strains positive in addition to one Bisgaard taxon 16 strain. Only the kmt1 method detected P. multocida with the expected sensitivity and specificity and this method is recommended for identification of the species.

U2 - 10.2376/0005-9366-126-415

DO - 10.2376/0005-9366-126-415

M3 - Journal article

SN - 0005-9366

VL - 126

SP - 415

EP - 422

JO - Berliner und Muenchener Tieraerztliche Wochenschrift

JF - Berliner und Muenchener Tieraerztliche Wochenschrift

IS - 9/10

ER -

Comparative study of PCR methods to detect Pasteurella multocida

Abstract

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