Comparative protection of two different commercial vaccines against Yersinia ruckeri serotype O1 and biotype 2 in rainbow trout (Oncorhynchus mykiss)

TY - JOUR

T1 - Comparative protection of two different commercial vaccines against Yersinia ruckeri serotype O1 and biotype 2 in rainbow trout (Oncorhynchus mykiss)

AU - Deshmukh, Sidhartha

AU - Raida, Martin Kristian

AU - Dalsgaard, Inger

AU - Chettri, Jiwan Kumar

AU - Kania, Per Walter

AU - Buchmann, Kurt

PY - 2012/1/15

Y1 - 2012/1/15

N2 - Differentially extended specific protection by two commercial vaccines against Yersinia ruckeri serotype O1 biotype 2 was studied following 30s immersion exposure. Rainbow trout were challenged intra-peritoneally (i.p.) with Y. ruckeri serotype O1, biotype 2 (≈10 6 to 10 7CFU/fish) at 4, 6 and 8 months after vaccination with vaccines containing either biotype 1 (AquaVac ® ERM) or both biotypes 1 and 2 (AquaVac ® RELERA™). The specific pattern of vaccine-mediated protection was evaluated by relative percentage survival (RPS) analysis at 4 and 6 months post-vaccination and by obtaining gross pathological observations at 4 and 8 months respectively. We determined specific significant and superior protection in terms of increased survivability in AquaVac ® RELERA™ vaccinated fish and observed correspondingly fewer pathological changes. The challenge trials indicated a longer protection for at least 6 months without any booster vaccination. A specific and adaptive response induced by AquaVac ® RELERA™ vaccine against Y. ruckeri biotype 2 was clearly indicated. In addition, some degree of cross protection rendered by AquaVac ® ERM containing biotype 1 during infection with Y. ruckeri biotype 2 was also noted.

AB - Differentially extended specific protection by two commercial vaccines against Yersinia ruckeri serotype O1 biotype 2 was studied following 30s immersion exposure. Rainbow trout were challenged intra-peritoneally (i.p.) with Y. ruckeri serotype O1, biotype 2 (≈10 6 to 10 7CFU/fish) at 4, 6 and 8 months after vaccination with vaccines containing either biotype 1 (AquaVac ® ERM) or both biotypes 1 and 2 (AquaVac ® RELERA™). The specific pattern of vaccine-mediated protection was evaluated by relative percentage survival (RPS) analysis at 4 and 6 months post-vaccination and by obtaining gross pathological observations at 4 and 8 months respectively. We determined specific significant and superior protection in terms of increased survivability in AquaVac ® RELERA™ vaccinated fish and observed correspondingly fewer pathological changes. The challenge trials indicated a longer protection for at least 6 months without any booster vaccination. A specific and adaptive response induced by AquaVac ® RELERA™ vaccine against Y. ruckeri biotype 2 was clearly indicated. In addition, some degree of cross protection rendered by AquaVac ® ERM containing biotype 1 during infection with Y. ruckeri biotype 2 was also noted.

KW - Former LIFE faculty

KW - Rainbow trout

KW - Yersinia ruckeri serotype O1, biotype 2

KW - Vaccine

KW - RPS

KW - Pathology

U2 - 10.1016/j.vetimm.2011.12.014

DO - 10.1016/j.vetimm.2011.12.014

M3 - Journal article

C2 - 22227075

SN - 0165-2427

VL - 145

SP - 379

EP - 385

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

IS - 1-2

ER -