Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes

Fotini A. Trikka; Alexandros Nikolaidis; Codruta Ignea; Aphrodite Tsaballa; Leto-Aikaterini Tziveleka; Efstathia Ioannou; Vassilios Roussis; Eleni A. Stea; Dragana Božić; Anagnostis Argiriou; Angelos K. Kanellis; Sotirios C. Kampranis; Antonios M. Makris

doi:10.1186/s12864-015-2147-3

Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes

Fotini A. Trikka, Alexandros Nikolaidis, Codruta Ignea, Aphrodite Tsaballa, Leto-Aikaterini Tziveleka, Efstathia Ioannou, Vassilios Roussis, Eleni A. Stea, Dragana Božić, Anagnostis Argiriou, Angelos K. Kanellis, Sotirios C. Kampranis, Antonios M. Makris

27 Citations (Scopus)

Abstract

BACKGROUND: Salvia diterpenes have been found to have health promoting properties. Among them, carnosic acid and carnosol, tanshinones and sclareol are well known for their cardiovascular, antitumor, antiinflammatory and antioxidant activities. However, many of these compounds are not available at a constant supply and developing biotechnological methods for their production could provide a sustainable alternative. The transcriptome of S.pomifera glandular trichomes was analysed aiming to identify genes that could be used in the engineering of synthetic microbial systems.

RESULTS: In the present study, a thorough metabolite analysis of S. pomifera leaves led to the isolation and structure elucidation of carnosic acid-family metabolites including one new natural product. These labdane diterpenes seem to be synthesized through miltiradiene and ferruginol. Transcriptomic analysis of the glandular trichomes from the S. pomifera leaves revealed two genes likely involved in miltiradiene synthesis. Their products were identified and the corresponding enzymes were characterized as copalyl diphosphate synthase (SpCDS) and miltiradiene synthase (SpMilS). In addition, several CYP-encoding transcripts were identified providing a valuable resource for the identification of the biosynthetic mechanism responsible for the production of carnosic acid-family metabolites in S. pomifera.

CONCLUSIONS: Our work has uncovered the key enzymes involved in miltiradiene biosynthesis in S. pomifera leaf glandular trichomes. The transcriptomic dataset obtained provides a valuable tool for the identification of the CYPs involved in the synthesis of carnosic acid-family metabolites.

Original language	English
Article number	935
Journal	BMC Genomics
Volume	16
Number of pages	19
ISSN	1471-2164
DOIs	https://doi.org/10.1186/s12864-015-2147-3
Publication status	Published - 2015
Externally published	Yes

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Trikka, F. A., Nikolaidis, A., Ignea, C., Tsaballa, A., Tziveleka, L.-A., Ioannou, E., Roussis, V., Stea, E. A., Božić, D., Argiriou, A., Kanellis, A. K., Kampranis, S. C., & Makris, A. M. (2015). Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes. BMC Genomics, 16, Article 935. https://doi.org/10.1186/s12864-015-2147-3

Trikka, FA, Nikolaidis, A, Ignea, C, Tsaballa, A, Tziveleka, L-A, Ioannou, E, Roussis, V, Stea, EA, Božić, D, Argiriou, A, Kanellis, AK, Kampranis, SC & Makris, AM 2015, 'Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes', BMC Genomics, vol. 16, 935. https://doi.org/10.1186/s12864-015-2147-3

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title = "Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes",

abstract = "BACKGROUND: Salvia diterpenes have been found to have health promoting properties. Among them, carnosic acid and carnosol, tanshinones and sclareol are well known for their cardiovascular, antitumor, antiinflammatory and antioxidant activities. However, many of these compounds are not available at a constant supply and developing biotechnological methods for their production could provide a sustainable alternative. The transcriptome of S.pomifera glandular trichomes was analysed aiming to identify genes that could be used in the engineering of synthetic microbial systems.RESULTS: In the present study, a thorough metabolite analysis of S. pomifera leaves led to the isolation and structure elucidation of carnosic acid-family metabolites including one new natural product. These labdane diterpenes seem to be synthesized through miltiradiene and ferruginol. Transcriptomic analysis of the glandular trichomes from the S. pomifera leaves revealed two genes likely involved in miltiradiene synthesis. Their products were identified and the corresponding enzymes were characterized as copalyl diphosphate synthase (SpCDS) and miltiradiene synthase (SpMilS). In addition, several CYP-encoding transcripts were identified providing a valuable resource for the identification of the biosynthetic mechanism responsible for the production of carnosic acid-family metabolites in S. pomifera.CONCLUSIONS: Our work has uncovered the key enzymes involved in miltiradiene biosynthesis in S. pomifera leaf glandular trichomes. The transcriptomic dataset obtained provides a valuable tool for the identification of the CYPs involved in the synthesis of carnosic acid-family metabolites.",

author = "Trikka, {Fotini A.} and Alexandros Nikolaidis and Codruta Ignea and Aphrodite Tsaballa and Leto-Aikaterini Tziveleka and Efstathia Ioannou and Vassilios Roussis and Stea, {Eleni A.} and Dragana Bo{\v z}i{\'c} and Anagnostis Argiriou and Kanellis, {Angelos K.} and Kampranis, {Sotirios C.} and Makris, {Antonios M.}",

year = "2015",

doi = "10.1186/s12864-015-2147-3",

language = "English",

volume = "16",

journal = "BMC Genomics",

issn = "1471-2164",

publisher = "BioMed Central Ltd.",

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TY - JOUR

T1 - Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes

AU - Trikka, Fotini A.

AU - Nikolaidis, Alexandros

AU - Ignea, Codruta

AU - Tsaballa, Aphrodite

AU - Tziveleka, Leto-Aikaterini

AU - Ioannou, Efstathia

AU - Roussis, Vassilios

AU - Stea, Eleni A.

AU - Božić, Dragana

AU - Argiriou, Anagnostis

AU - Kanellis, Angelos K.

AU - Kampranis, Sotirios C.

AU - Makris, Antonios M.

PY - 2015

Y1 - 2015

N2 - BACKGROUND: Salvia diterpenes have been found to have health promoting properties. Among them, carnosic acid and carnosol, tanshinones and sclareol are well known for their cardiovascular, antitumor, antiinflammatory and antioxidant activities. However, many of these compounds are not available at a constant supply and developing biotechnological methods for their production could provide a sustainable alternative. The transcriptome of S.pomifera glandular trichomes was analysed aiming to identify genes that could be used in the engineering of synthetic microbial systems.RESULTS: In the present study, a thorough metabolite analysis of S. pomifera leaves led to the isolation and structure elucidation of carnosic acid-family metabolites including one new natural product. These labdane diterpenes seem to be synthesized through miltiradiene and ferruginol. Transcriptomic analysis of the glandular trichomes from the S. pomifera leaves revealed two genes likely involved in miltiradiene synthesis. Their products were identified and the corresponding enzymes were characterized as copalyl diphosphate synthase (SpCDS) and miltiradiene synthase (SpMilS). In addition, several CYP-encoding transcripts were identified providing a valuable resource for the identification of the biosynthetic mechanism responsible for the production of carnosic acid-family metabolites in S. pomifera.CONCLUSIONS: Our work has uncovered the key enzymes involved in miltiradiene biosynthesis in S. pomifera leaf glandular trichomes. The transcriptomic dataset obtained provides a valuable tool for the identification of the CYPs involved in the synthesis of carnosic acid-family metabolites.

AB - BACKGROUND: Salvia diterpenes have been found to have health promoting properties. Among them, carnosic acid and carnosol, tanshinones and sclareol are well known for their cardiovascular, antitumor, antiinflammatory and antioxidant activities. However, many of these compounds are not available at a constant supply and developing biotechnological methods for their production could provide a sustainable alternative. The transcriptome of S.pomifera glandular trichomes was analysed aiming to identify genes that could be used in the engineering of synthetic microbial systems.RESULTS: In the present study, a thorough metabolite analysis of S. pomifera leaves led to the isolation and structure elucidation of carnosic acid-family metabolites including one new natural product. These labdane diterpenes seem to be synthesized through miltiradiene and ferruginol. Transcriptomic analysis of the glandular trichomes from the S. pomifera leaves revealed two genes likely involved in miltiradiene synthesis. Their products were identified and the corresponding enzymes were characterized as copalyl diphosphate synthase (SpCDS) and miltiradiene synthase (SpMilS). In addition, several CYP-encoding transcripts were identified providing a valuable resource for the identification of the biosynthetic mechanism responsible for the production of carnosic acid-family metabolites in S. pomifera.CONCLUSIONS: Our work has uncovered the key enzymes involved in miltiradiene biosynthesis in S. pomifera leaf glandular trichomes. The transcriptomic dataset obtained provides a valuable tool for the identification of the CYPs involved in the synthesis of carnosic acid-family metabolites.

U2 - 10.1186/s12864-015-2147-3

DO - 10.1186/s12864-015-2147-3

M3 - Journal article

C2 - 26572682

SN - 1471-2164

VL - 16

JO - BMC Genomics

JF - BMC Genomics

M1 - 935

ER -

Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes

Abstract

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