TY - JOUR
T1 - Colostrum and amniotic fluid from different species exhibit similar immunomodulating effects in bacterium-stimulated dendritic cells
AU - Møller, Hanne Kristine
AU - Fink, Lisbeth N.
AU - Sangild, Per Torp
AU - Frøkiær, Hanne
PY - 2011/11/1
Y1 - 2011/11/1
N2 - The fetus and newborn are immunologically immature. Bioactive compounds in amniotic fluid (AF) and maternal milk therefore play a key role in the immunological development of the infant intestine. We hypothesized that colostrum and AF exert similar immunomodulatory effects on the developing immune system. Hence, bone marrow-derived murine dendritic cells (BMDCs) were co-incubated with Clostridium perfringens A or Escherichia coli Nissle 1917 and porcine, bovine, or human AF, colostrum/milk whey fractions. Interleukin (IL) 10, IL-12, IL-6, and tumor necrosis factor-α (TNF-α) production was measured. IL-12 production was reduced with all AFs and wheys, and IL-6 and TNF-α were also reduced by porcine AF. Porcine and bovine whey both reduced TNF-α production. Overall, the reductions were most pronounced with the porcine fluids. Only bovine fluids caused strong induction of IL-10. Overall, effects of AF and whey from same species were similar. Viability of stimulated BMDCs was not significantly affected by the fluids. Neutralization of IL-10, transforming growth factor β, and epidermal growth factor (EGF) did not remove the IL-12-inhibiting effect, but EGF neutralization increased IL-10 production. Addition of EFG to DCs enhanced the bacterium-induced cytokine production contrary to the effect of AF and colostrums, ruling out EGF as the inhibitory component in the fluids.
AB - The fetus and newborn are immunologically immature. Bioactive compounds in amniotic fluid (AF) and maternal milk therefore play a key role in the immunological development of the infant intestine. We hypothesized that colostrum and AF exert similar immunomodulatory effects on the developing immune system. Hence, bone marrow-derived murine dendritic cells (BMDCs) were co-incubated with Clostridium perfringens A or Escherichia coli Nissle 1917 and porcine, bovine, or human AF, colostrum/milk whey fractions. Interleukin (IL) 10, IL-12, IL-6, and tumor necrosis factor-α (TNF-α) production was measured. IL-12 production was reduced with all AFs and wheys, and IL-6 and TNF-α were also reduced by porcine AF. Porcine and bovine whey both reduced TNF-α production. Overall, the reductions were most pronounced with the porcine fluids. Only bovine fluids caused strong induction of IL-10. Overall, effects of AF and whey from same species were similar. Viability of stimulated BMDCs was not significantly affected by the fluids. Neutralization of IL-10, transforming growth factor β, and epidermal growth factor (EGF) did not remove the IL-12-inhibiting effect, but EGF neutralization increased IL-10 production. Addition of EFG to DCs enhanced the bacterium-induced cytokine production contrary to the effect of AF and colostrums, ruling out EGF as the inhibitory component in the fluids.
U2 - 10.1089/jir.2010.0070
DO - 10.1089/jir.2010.0070
M3 - Journal article
C2 - 21777143
SN - 1079-9907
VL - 31
SP - 813
EP - 823
JO - Journal of Interferon and Cytokine Research
JF - Journal of Interferon and Cytokine Research
IS - 11
ER -