Colocalization of synapsin and actin during synaptic vesicle recycling.

TY - JOUR

T1 - Colocalization of synapsin and actin during synaptic vesicle recycling.

AU - Bloom, Ona

AU - Evergren, Emma

AU - Tomilin, Nikolay

AU - Kjaerulff, Ole

AU - Löw, Peter

AU - Brodin, Lennart

AU - Pieribone, Vincent A

AU - Greengard, Paul

AU - Shupliakov, Oleg

N1 - Keywords: Actins; Animals; Endocytosis; Lampreys; Microinjections; Microscopy, Immunoelectron; Models, Biological; Spinal Cord; Synapses; Synapsins; Synaptic Vesicles

PY - 2003

Y1 - 2003

N2 - It has been hypothesized that in the mature nerve terminal, interactions between synapsin and actin regulate the clustering of synaptic vesicles and the availability of vesicles for release during synaptic activity. Here, we have used immunogold electron microscopy to examine the subcellular localization of actin and synapsin in the giant synapse in lamprey at different states of synaptic activity. In agreement with earlier observations, in synapses at rest, synapsin immunoreactivity was preferentially localized to a portion of the vesicle cluster distal to the active zone. During synaptic activity, however, synapsin was detected in the pool of vesicles proximal to the active zone. In addition, actin and synapsin were found colocalized in a dynamic filamentous cytomatrix at the sites of synaptic vesicle recycling, endocytic zones. Synapsin immunolabeling was not associated with clathrin-coated intermediates but was found on vesicles that appeared to be recycling back to the cluster. Disruption of synapsin function by microinjection of antisynapsin antibodies resulted in a prominent reduction of the cytomatrix at endocytic zones of active synapses. Our data suggest that in addition to its known function in clustering of vesicles in the reserve pool, synapsin migrates from the synaptic vesicle cluster and participates in the organization of the actin-rich cytomatrix in the endocytic zone during synaptic activity.

AB - It has been hypothesized that in the mature nerve terminal, interactions between synapsin and actin regulate the clustering of synaptic vesicles and the availability of vesicles for release during synaptic activity. Here, we have used immunogold electron microscopy to examine the subcellular localization of actin and synapsin in the giant synapse in lamprey at different states of synaptic activity. In agreement with earlier observations, in synapses at rest, synapsin immunoreactivity was preferentially localized to a portion of the vesicle cluster distal to the active zone. During synaptic activity, however, synapsin was detected in the pool of vesicles proximal to the active zone. In addition, actin and synapsin were found colocalized in a dynamic filamentous cytomatrix at the sites of synaptic vesicle recycling, endocytic zones. Synapsin immunolabeling was not associated with clathrin-coated intermediates but was found on vesicles that appeared to be recycling back to the cluster. Disruption of synapsin function by microinjection of antisynapsin antibodies resulted in a prominent reduction of the cytomatrix at endocytic zones of active synapses. Our data suggest that in addition to its known function in clustering of vesicles in the reserve pool, synapsin migrates from the synaptic vesicle cluster and participates in the organization of the actin-rich cytomatrix in the endocytic zone during synaptic activity.

U2 - 10.1083/jcb.200212140

DO - 10.1083/jcb.200212140

M3 - Journal article

C2 - 12756235

SN - 0021-9525

VL - 161

SP - 737

EP - 747

JO - Journal of Cell Biology

JF - Journal of Cell Biology

IS - 4

ER -