Cohort description: The Danish blood donor staphylococcus aureus carriage study

Lise Tornvig Erikstrup; Khoa Manh Dinh; Paal Skytt Andersen; Robert Leo Skov; Kathrine Agergård Kaspersen; Kaspar René Nielsen; Svend Ellermann-Eriksen; Christian Erikstrup

doi:10.2147/CLEP.S218637

Cohort description: The Danish blood donor staphylococcus aureus carriage study

Lise Tornvig Erikstrup, Khoa Manh Dinh^*, Paal Skytt Andersen, Robert Leo Skov, Kathrine Agergård Kaspersen, Kaspar René Nielsen, Svend Ellermann-Eriksen, Christian Erikstrup

^*Corresponding author for this work

Food Safety and Zoonoses

5 Citations (Scopus)

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Abstract

Purpose: Staphylococcus aureus carriage poses an increased risk of S. aureus infection. The aim of this study was to investigate the colonization of S. aureus among healthy individuals and to establish a prospective cohort and biobank for research in the health consequences of colonization. Population and methods: The Danish Blood Donor S. aureus Carriage Study (DBDSaCS) was established in 2014. So far, a total of 6082 healthy participants have been included with nasal swabs and repeated swabs are performed at subsequent donations. Samples from the first 2217 participants were cultured using a two-step method to evaluate the effect of using enrichment broth. Furthermore, 262 participants were sampled from both the nares and the throat. All participants completed a questionnaire with self-reported health, anthropometric measurements, current smoking status, and physical activity. Plasma samples, nasal swab transport media, and S. aureus isolates were stored. Results: The prevalence of S. aureus nasal colonization was 41%. The prevalence of colonization was higher in men (46%) than women (34%), lower for smokers, and decreased with increasing age (<25 years: 44% vs >55 years: 35%). In participants swabbed from the nose and throat, the prevalence of S. aureus colonization after enrichment was 55% with significantly higher prevalence in the throat (45%) than in the nose (40%). The use of an enrichment broth increased the proportion of S. aureus colonization. Conclusion: We describe a large and growing cohort of healthy individuals established to investigate predictors for S. aureus carriage and the health consequences of carriage. Multiple projects using data from DBDSaCS linked with Danish health registers, biomarkers, and genetic markers are ongoing. Results will be published in the coming years.

Original language	English
Journal	Clinical Epidemiology
Volume	11
Pages (from-to)	885-900
Number of pages	16
ISSN	1179-1349
DOIs	https://doi.org/10.2147/CLEP.S218637
Publication status	Published - 2019

Keywords

Blood donor health
Colonization
Epidemiology
Prospective cohort study
Risk factors
Staphylococcus aureus

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Cohort description: The Danish Blood Donor Staphylococcus aureus Carriage StudyFinal published version, 2.09 MBLicence: CC BY-NC

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title = "Cohort description: The Danish blood donor staphylococcus aureus carriage study",

abstract = "Purpose: Staphylococcus aureus carriage poses an increased risk of S. aureus infection. The aim of this study was to investigate the colonization of S. aureus among healthy individuals and to establish a prospective cohort and biobank for research in the health consequences of colonization. Population and methods: The Danish Blood Donor S. aureus Carriage Study (DBDSaCS) was established in 2014. So far, a total of 6082 healthy participants have been included with nasal swabs and repeated swabs are performed at subsequent donations. Samples from the first 2217 participants were cultured using a two-step method to evaluate the effect of using enrichment broth. Furthermore, 262 participants were sampled from both the nares and the throat. All participants completed a questionnaire with self-reported health, anthropometric measurements, current smoking status, and physical activity. Plasma samples, nasal swab transport media, and S. aureus isolates were stored. Results: The prevalence of S. aureus nasal colonization was 41%. The prevalence of colonization was higher in men (46%) than women (34%), lower for smokers, and decreased with increasing age (<25 years: 44% vs >55 years: 35%). In participants swabbed from the nose and throat, the prevalence of S. aureus colonization after enrichment was 55% with significantly higher prevalence in the throat (45%) than in the nose (40%). The use of an enrichment broth increased the proportion of S. aureus colonization. Conclusion: We describe a large and growing cohort of healthy individuals established to investigate predictors for S. aureus carriage and the health consequences of carriage. Multiple projects using data from DBDSaCS linked with Danish health registers, biomarkers, and genetic markers are ongoing. Results will be published in the coming years.",

keywords = "Blood donor health, Colonization, Epidemiology, Prospective cohort study, Risk factors, Staphylococcus aureus",

author = "Erikstrup, {Lise Tornvig} and Dinh, {Khoa Manh} and Andersen, {Paal Skytt} and Skov, {Robert Leo} and Kaspersen, {Kathrine Agerg{\aa}rd} and Nielsen, {Kaspar Ren{\'e}} and Svend Ellermann-Eriksen and Christian Erikstrup",

year = "2019",

doi = "10.2147/CLEP.S218637",

language = "English",

volume = "11",

pages = "885--900",

journal = "Clinical Epidemiology",

issn = "1179-1349",

publisher = "Dove Medical Press Ltd",

}

TY - JOUR

T1 - Cohort description

T2 - The Danish blood donor staphylococcus aureus carriage study

AU - Erikstrup, Lise Tornvig

AU - Dinh, Khoa Manh

AU - Andersen, Paal Skytt

AU - Skov, Robert Leo

AU - Kaspersen, Kathrine Agergård

AU - Nielsen, Kaspar René

AU - Ellermann-Eriksen, Svend

AU - Erikstrup, Christian

PY - 2019

Y1 - 2019

N2 - Purpose: Staphylococcus aureus carriage poses an increased risk of S. aureus infection. The aim of this study was to investigate the colonization of S. aureus among healthy individuals and to establish a prospective cohort and biobank for research in the health consequences of colonization. Population and methods: The Danish Blood Donor S. aureus Carriage Study (DBDSaCS) was established in 2014. So far, a total of 6082 healthy participants have been included with nasal swabs and repeated swabs are performed at subsequent donations. Samples from the first 2217 participants were cultured using a two-step method to evaluate the effect of using enrichment broth. Furthermore, 262 participants were sampled from both the nares and the throat. All participants completed a questionnaire with self-reported health, anthropometric measurements, current smoking status, and physical activity. Plasma samples, nasal swab transport media, and S. aureus isolates were stored. Results: The prevalence of S. aureus nasal colonization was 41%. The prevalence of colonization was higher in men (46%) than women (34%), lower for smokers, and decreased with increasing age (<25 years: 44% vs >55 years: 35%). In participants swabbed from the nose and throat, the prevalence of S. aureus colonization after enrichment was 55% with significantly higher prevalence in the throat (45%) than in the nose (40%). The use of an enrichment broth increased the proportion of S. aureus colonization. Conclusion: We describe a large and growing cohort of healthy individuals established to investigate predictors for S. aureus carriage and the health consequences of carriage. Multiple projects using data from DBDSaCS linked with Danish health registers, biomarkers, and genetic markers are ongoing. Results will be published in the coming years.

AB - Purpose: Staphylococcus aureus carriage poses an increased risk of S. aureus infection. The aim of this study was to investigate the colonization of S. aureus among healthy individuals and to establish a prospective cohort and biobank for research in the health consequences of colonization. Population and methods: The Danish Blood Donor S. aureus Carriage Study (DBDSaCS) was established in 2014. So far, a total of 6082 healthy participants have been included with nasal swabs and repeated swabs are performed at subsequent donations. Samples from the first 2217 participants were cultured using a two-step method to evaluate the effect of using enrichment broth. Furthermore, 262 participants were sampled from both the nares and the throat. All participants completed a questionnaire with self-reported health, anthropometric measurements, current smoking status, and physical activity. Plasma samples, nasal swab transport media, and S. aureus isolates were stored. Results: The prevalence of S. aureus nasal colonization was 41%. The prevalence of colonization was higher in men (46%) than women (34%), lower for smokers, and decreased with increasing age (<25 years: 44% vs >55 years: 35%). In participants swabbed from the nose and throat, the prevalence of S. aureus colonization after enrichment was 55% with significantly higher prevalence in the throat (45%) than in the nose (40%). The use of an enrichment broth increased the proportion of S. aureus colonization. Conclusion: We describe a large and growing cohort of healthy individuals established to investigate predictors for S. aureus carriage and the health consequences of carriage. Multiple projects using data from DBDSaCS linked with Danish health registers, biomarkers, and genetic markers are ongoing. Results will be published in the coming years.

KW - Blood donor health

KW - Colonization

KW - Epidemiology

KW - Prospective cohort study

KW - Risk factors

KW - Staphylococcus aureus

U2 - 10.2147/CLEP.S218637

DO - 10.2147/CLEP.S218637

M3 - Journal article

C2 - 31572018

AN - SCOPUS:85073290189

SN - 1179-1349

VL - 11

SP - 885

EP - 900

JO - Clinical Epidemiology

JF - Clinical Epidemiology

ER -

Cohort description: The Danish blood donor staphylococcus aureus carriage study

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Keywords

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