Co-expression of mCysLT1 receptors and IK channels in Xenopus laevis oocytes elicits LTD4-stimulated IK current, independent of an increase in [Ca2+]i.

Tune Wulff; Charlotte Hougaard; Dan A Klaerke; Else K Hoffmann

doi:10.1016/j.bbamem.2003.11.001

Co-expression of mCysLT1 receptors and IK channels in Xenopus laevis oocytes elicits LTD4-stimulated IK current, independent of an increase in [Ca2+]i.

Tune Wulff, Charlotte Hougaard, Dan A Klaerke, Else K Hoffmann

Cell Biology and Physiology

1 Citation (Scopus)

Abstract

Addition of LTD4 (10 nM) to Xenopus laevis oocytes expressing the mCysLT1 receptor together with hBK or hIK channels resulted in the activation of both channels secondary to an LTD4-induced increase in [Ca2+]i. In addition, the hIK channel is activated by low concentrations of LTD4 (<0.1 nM), which did not result in any increase in [Ca2+]i. Even though activation of hIK by low concentrations of LTD4 was independent of an increase in [Ca2+]i, a certain "permissive" level of [Ca2+]i was required for its activation, since buffering of intracellular Ca2+ by EGTA completely abolished the response to LTD4. Neither hTBAK1 nor hTASK2 was activated following stimulations with LTD4 (0.1 and 100 nM).

Original language	English
Journal	BBA General Subjects
Volume	1660
Issue number	1-2
Pages (from-to)	75-9
Number of pages	4
ISSN	0304-4165
DOIs	https://doi.org/10.1016/j.bbamem.2003.11.001
Publication status	Published - 2004

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@article{5b171780a0f311dd86a6000ea68e967b,

title = "Co-expression of mCysLT1 receptors and IK channels in Xenopus laevis oocytes elicits LTD4-stimulated IK current, independent of an increase in [Ca2+]i.",

abstract = "Addition of LTD4 (10 nM) to Xenopus laevis oocytes expressing the mCysLT1 receptor together with hBK or hIK channels resulted in the activation of both channels secondary to an LTD4-induced increase in [Ca2+]i. In addition, the hIK channel is activated by low concentrations of LTD4 (<0.1 nM), which did not result in any increase in [Ca2+]i. Even though activation of hIK by low concentrations of LTD4 was independent of an increase in [Ca2+]i, a certain {"}permissive{"} level of [Ca2+]i was required for its activation, since buffering of intracellular Ca2+ by EGTA completely abolished the response to LTD4. Neither hTBAK1 nor hTASK2 was activated following stimulations with LTD4 (0.1 and 100 nM).",

author = "Tune Wulff and Charlotte Hougaard and Klaerke, {Dan A} and Hoffmann, {Else K}",

note = "Keywords: Animals; Calcium; Cations, Divalent; Cell Line; Cytokines; Egtazic Acid; Humans; Hydrogen-Ion Concentration; Large-Conductance Calcium-Activated Potassium Channels; Leukotriene D4; Membrane Proteins; Oocytes; Potassium Channels; Potassium Channels, Calcium-Activated; Potassium Channels, Tandem Pore Domain; RNA, Complementary; Receptors, Leukotriene; Transfection; Xenopus laevis",

year = "2004",

doi = "10.1016/j.bbamem.2003.11.001",

language = "English",

volume = "1660",

pages = "75--9",

journal = "B B A - General Subjects",

issn = "0304-4165",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Co-expression of mCysLT1 receptors and IK channels in Xenopus laevis oocytes elicits LTD4-stimulated IK current, independent of an increase in [Ca2+]i.

AU - Wulff, Tune

AU - Hougaard, Charlotte

AU - Klaerke, Dan A

AU - Hoffmann, Else K

N1 - Keywords: Animals; Calcium; Cations, Divalent; Cell Line; Cytokines; Egtazic Acid; Humans; Hydrogen-Ion Concentration; Large-Conductance Calcium-Activated Potassium Channels; Leukotriene D4; Membrane Proteins; Oocytes; Potassium Channels; Potassium Channels, Calcium-Activated; Potassium Channels, Tandem Pore Domain; RNA, Complementary; Receptors, Leukotriene; Transfection; Xenopus laevis

PY - 2004

Y1 - 2004

N2 - Addition of LTD4 (10 nM) to Xenopus laevis oocytes expressing the mCysLT1 receptor together with hBK or hIK channels resulted in the activation of both channels secondary to an LTD4-induced increase in [Ca2+]i. In addition, the hIK channel is activated by low concentrations of LTD4 (<0.1 nM), which did not result in any increase in [Ca2+]i. Even though activation of hIK by low concentrations of LTD4 was independent of an increase in [Ca2+]i, a certain "permissive" level of [Ca2+]i was required for its activation, since buffering of intracellular Ca2+ by EGTA completely abolished the response to LTD4. Neither hTBAK1 nor hTASK2 was activated following stimulations with LTD4 (0.1 and 100 nM).

AB - Addition of LTD4 (10 nM) to Xenopus laevis oocytes expressing the mCysLT1 receptor together with hBK or hIK channels resulted in the activation of both channels secondary to an LTD4-induced increase in [Ca2+]i. In addition, the hIK channel is activated by low concentrations of LTD4 (<0.1 nM), which did not result in any increase in [Ca2+]i. Even though activation of hIK by low concentrations of LTD4 was independent of an increase in [Ca2+]i, a certain "permissive" level of [Ca2+]i was required for its activation, since buffering of intracellular Ca2+ by EGTA completely abolished the response to LTD4. Neither hTBAK1 nor hTASK2 was activated following stimulations with LTD4 (0.1 and 100 nM).

U2 - 10.1016/j.bbamem.2003.11.001

DO - 10.1016/j.bbamem.2003.11.001

M3 - Journal article

C2 - 14757222

SN - 0304-4165

VL - 1660

SP - 75

EP - 79

JO - B B A - General Subjects

JF - B B A - General Subjects

IS - 1-2

ER -

Co-expression of mCysLT1 receptors and IK channels in Xenopus laevis oocytes elicits LTD4-stimulated IK current, independent of an increase in [Ca2+]i.

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